identify one gene (other than myosin heavy chain) that is upregulated during C2C12 cell differentiation. Describe...
identify one gene (other than myosin heavy chain) that is upregulated during C2C12 cell differentiation. Describe the experimental evidence supporting an increased expression of this gene during differentiation. What is the purpose of this gene and how does it make C2C12 cells more specialized? Explain.
Homework Answers
c6orf32 (LOCATED ON CHROMOSOME 6) IS UPREGULATED DURING MUSCLE CELL DIFFERENTIATION.
mRNA KNOCKDOWN EXPERIMENTS IN THE C2C12 MURINE MYOBLAST CELL LINE DEMONSTRATED THAT CELLS LACKING c6orf32 EXHIBIT A MYOGENIC DIFFERENTIATION DEFECT, CHARACTERIZED BY DECREASE IN EXPRESSION OF MYOGENIN AND MHC(MYOSIN HEAVY CHAIN) PROTEINS, WHEREAS MyoD1 WAS UNALTERED. IN CONTRAST, OVEREXPRESSION OF c6orf32 IN C2C12 CELLS PROMOTED FORMATION OF LONG MEMBRANE PROTRUSIONS(FILOPODIA).
PURPOSE OF c6orf32:- IT IS SUGGESTED THAT pl48 (SHORT ISOFORM OF c6orf32) PLAYS A IMPORTANT ROLE IN FORMATION OF NON-MITOTIC MULTINUCLEATED SYNCYTIUM DURING PLANCENTAL DIFFERENTIAL. AMINO ACIDS 55-113 OF C6ORF32 IS LIKELY INVOLVED IN THE FORMATION OF FILOPODIA. TOGETHER THESE RESULTS SUGGESTS THAT C6ORF32 PLAY MULTIPLE ROLES DURING CELL DIFFERENTIATION BY MEDIATING FILOPODIA FORMATION, CYTOSKELETON REARRANGEMENT AND BY INFLUENCING EITHER DIRECTLY OR INDIRECTLY, THE PROGRESSION OF MYOGENIC DIFFERENTIATION. HENCE , MAKING C2C12 CELLS MORE SPECIALIZED.
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