cDNA synthesis uses primers that are
1. polyT
2. polyA
3. RNA
4. cDNA
cDNA synthesis uses primers that are 1. polyT 2. polyA 3. RNA 4. cDNA
Polyadenylation of RNA species is an important criterion for the production of cDNA species. Which of the following holds true?a) Polyadenylation should be at 3’ endb) Eukaryotic mRNAs are mostly non-polyadenylatedc) Bacterial mRNAs and organelle mRNAs are polyadenylatedd) It is carried out by the addition of T residues after synthesis
Question 3 1 pts The RNA type directly involved in protein synthesis is short interfering RNA. long nc RNA. ribosomal RNA. small nuclear RNA. Question 4 1 pts Which class of RNA molecules is unique to eukaryotic organisms? O transfer RNA ribosomal RNA O messenger RNA small nuclear RNA
1) Which of the following statements is NOT true regarding cDNA? a. cDNA contains the same four nucleotides (A, T, G, C) as chromosome DNA b. cDNA is made using RNA as a template c. cDNA is found in both eukaryotic and bacterial cells d. cDNA stands for "complementary DNA" 2) The PCR technique amplifies random regions of the genome a. True b. False 3) Which of the nucleotide sequences below is most likely to be cut by a restriction...
TISL slalu SHLIC33 (CDNA SYU 1. Dilute 1 ug of total template RNA to a final volume of 26.25 pl with RNase- free ddH2O in a 0.7 ml microcentrifuge tube. Denature the RNA by heating at 65°C for 5 minutes. Store on ice (Why are you denaturing the RNA?). ng/uL 741.2
Order the steps in RT-PCR: 1. Add one primer complementary to the 3' end of the RNA of interest. Also add the RT enzyme (reverse transcriptase), dNTPs, and a buffer with Mg2+. Incubate the reaction appropriately. 2. Degrade the RNA strand with base, leaving just the cDNA. 3. cDNA is produced, which is single-stranded and complementary to the RNA of interest. 4. Double-stranded DNA for the region of interest is produced. 5. Add two primers (one forward and one reverse)...
3’-TATAAAGACTTACAAATTTGTCCCCATTTTGC-5’ 5’-ATATTTCTGAATGTTTAAACAGGGGTAAAACG-3’ a. Diagram the results you would obtain for 1 and 2 rounds of PCR replication using the primers, 5’-ATGTT-3’ and 3’-CCATT-5’ and template above. b. The primers in part b were designed to make it easy to illustrate the PCR process, in practice PCR primers are 18-25bp in length. Why do PCR primers that are 5bp fail? c. Are primers used in PCR RNA or DNA? d. Explain how PCR amplifies the gene of interest without amplifying the...
Question 2 Select the statement below that BEST describes the synthesis of cDNA. mRNA is used as a template strand to guide synthesis specific sequences of DNA are localized and quantified within a genome numerous DNA copies are made of a specific segment of DNA restriction enzymes are used to generate sticky ends on the gene of interest as well as the plasmid vector
There are similarities between DNA synthesis and RNA synthesis in prokaryotes. Compare and contrast the following items. Write in logical organization. List 4 ways to show the different properties of DNA polymerase and RNA polymerase. (4 marks) 1.
Please answer 1.) During translation t-RNA carrying new amino acids enters ribosome site of A.) P B.)E C.) A D.) any of these 2.) Split genes have coding parts: A.) exons B.) introns C.) replicons D.) promoters 3.) Synthesis of cDNA from m-RNA is A.) transcription translation B.) translation C.) reverse transcription D.) reverse 4.) In electrophoresis, DNA fragments will move towards: A.) +electrode movement B.)-electrode C.) both +& - electrode D.) no 5.) Synthesis of DNA takes place against...
The schematic illustrates steps in RNA processing. The DNA strand is shown in red and yellow, and the RNA strand is shown in green and blue. The steps in RNA processing are labeled 1-3, and the components of the RNA transcript are labeled A-C. Steps in RNA processing are indicated below in alphabetical order, select the step number to put these processes in chronological order. If the indicated step does not occur, select 'This process does not happen'. removal of...