Question

1) Which of the following statements is NOT true regarding cDNA?

a. cDNA contains the same four nucleotides (A, T, G, C) as chromosome DNA

b. cDNA is made using RNA as a template

c. cDNA is found in both eukaryotic and bacterial cells

d. cDNA stands for "complementary DNA"

2) The PCR technique amplifies random regions of the genome

a. True

b. False

3) Which of the nucleotide sequences below is most likely to be cut by a restriction enzyme?

a. 5' ATACCGAC 3'

b. 5' ATGCGCAT 3'

c. 5' AAAAAAAA 3'

d. 5' UUCGAUCG 3'

Answer 1

1. Answer: c. cDNA is found in both eukaryotic and bacterial cells

This statement is not true, cDNA is mainly naturally found in retroviruses. cDNA is often made from mRNA for the ease of cloning, as it contains only exons and no introns. But cDNA is not naturally present in most cells.

2. The PCR technique amplifies random regions of the genome - This is False.

PCR is used to amplify specific regions of the genome using primers specific to certain region of the genome.

3. Restrction enzymes generally recogize palindromic sequences in the DNA . This means the sequence is read same in the reverse starnd of the DNA also.

b. 5' ATGCGCAT 3' - is a palindromic sequence so it is most likely to be cut by restriction enzyme.