Question

what method will you use to monitor melting and annealing without chemically destroying DNA?

Answer 1

We can use a simple method of determining the absorbance of DNA.

The double stranded DNA which is in its intact form has a lesser value of absorbance as compared to two single stranded DNA which is formed after denaturation of double stranded DNA.

Denaturation is the process of breaking down of double stranded DNA into single standard form by disrupting the hydrogen bond present between complementary base pairs. Hydrogen bonds are responsible for keeping the two strands of DNA associated with each other. The process of denaturation can be carried out at high temperature or using denaturing agents like urea.

Denaturation is a reversible process and its reverse is known as renaturation. It occurs when the temperature is returned back to the normal value and denaturing agent are also removed. Renaturation is the formation of double stranded DNA molecule by Association of single stranded DNA molecule with the help of hydrogen bonding.

It should be noted that DNA denaturation is not a chemical process. It is a reversible and physical process.

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