how much monosodium phosphate and disodium phosphate (g) are needed to make 1000 mL of 250...
how much monosodium phosphate and disodium phosphate (g) are needed to make 1000 mL of 250 mmolar buffer at pH 6.5
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how much monosodium phosphate and disodium phosphate (g) are
needed to make 1000 mL of 250...
Calculate the concentration of disodium phosphate (Na2HPO4) and monosodium phosphate (NaH2PO4) to prepare of 100.0mM buffer...
Calculate the concentration of disodium phosphate (Na2HPO4) and monosodium phosphate (NaH2PO4) to prepare of 100.0mM buffer at pH 7.50. the pKa of H2PO4= 7.21
a 50 mM buffer of monosodium dihydrogen phosphate and disodium monohydrogen phosphate at pH 7. The...
a 50 mM buffer of monosodium dihydrogen phosphate and disodium monohydrogen phosphate at pH 7. The relevant pKa is 6.8. You'll have to figure out the concentrations of all the ionic species before computing the ionic strength. -Solve the problem using the Henderson-Hasselbalch equation to find the concentrations, use the two equations with two unknowns to solve. HPO4 = 0.031M, and H2PO4=0.019M. Then do the ionic strength. It's supposed to be u=0.096M, but I don't understand how this works. I'm...
Throughout the table shown below, explain how you'll prepare a liter of phosphate buffer at a...
Throughout the table shown below, explain how you'll prepare a liter of phosphate buffer at a pH of 7.402 Phosphoric acid (H3PO4) Monosodium phosphate (NaH2PO4) Disodium phosphate (Na2HPO4) pKai = 2.16 pKaz = 7.21 pKaz = 12.32
What masses of monobasic and dibasic sodium phosphate will you use to make 250 mL of...
What masses of monobasic and dibasic sodium phosphate will you use to make 250 mL of 0.1 M sodium phosphate buffer, pH = 7?
answer question 3and 4 Question 2 (2 points) If 0.25 g of Na2HPO4 (disodium phosphate) is...
answer question 3and 4
Question 2 (2 points) If 0.25 g of Na2HPO4 (disodium phosphate) is added to the 50 mL 0.30 M KH2PO4 solution, calculate the pH of the solution. Question 3 (2 points) Calculate the volume (in L) of 0.50 M NH4Cl to add to 50.00 ml of 1.0 M NH3 to preparea buffer at pH 9.00. Question 4 (2 points) Use the Henderson Hasselbach equation to calculate the pH of the solution which contains 10 mL of...
4. How much of (in mL) 0.15 M acetic acid (pKa 4.76) is needed to mix...
4. How much of (in mL) 0.15 M acetic acid (pKa 4.76) is needed to mix with 40 mL of 0.15 M sodium acetate to make a buffer of pH = 4.50 of 250 mL? (molar mass of acetic acid and sodium acetate are 60.0 g/mol and 82.0 g/ml, pKa of acetic acid is 4.76) (extra 10 pt)
3. How would you make 200 ml of a 250 mM phosphate buffer from a 1M...
3. How would you make 200 ml of a 250 mM phosphate buffer from a 1M stock solution of phosphate buffer? r. nus: how would you make 100 ul of 10 mM glucose solution from a 1M glucose stock solution? int: might need to do a serial dilution, as our pipettes cannot dispense less than 2ul)
(1) When calibrating a glass electrode, 0.025 M potassium dihydrogen phosphate/0.025 M disodium hydrogen phosphate buffer...
(1) When calibrating a glass electrode, 0.025 M potassium dihydrogen phosphate/0.025 M disodium hydrogen phosphate buffer (pH = 6.881) gave a reading of -18.3 mV at 20 °C and 0.050 M potassium hydrogen phthalate buffer (pH = 3.788) gave a reading of +146.3 mV. What is the pH of an unknown giving a reading of +50.0 mV? What is the slope of the calibration curve (mV/pH units) and what is the theoretical slope at 20 °C? Find the value of...
Solutions for DNA cloning and DNA analysis a. 0.5M EDTA (disodium ethylenediaminetetra-acetate; t...
Show calculations for the solutions.
Solutions for DNA cloning and DNA analysis a. 0.5M EDTA (disodium ethylenediaminetetra-acetate; tw 372.2), pH8.0 prepare 50mL hint: the disodium salt of EDTA will not go into solution until the pH of the solution is close to 8.0. To accomplish this, you will need to add approximately 2g of NaOH pellets b. 50XTAE (Tris-acetate/EDTA) (working solution at 1x is 0.04M Tris-acetate, 0.001M EDTA) Make 100mL 2M Tris base (fw 121.1) 5.71mL glacial acetic acid 0.05M...
Describe how you would prepare 250 ml of 0.2M phosphate buffer, pH =12.5, given solid Na2HPO4.2H2O...
Describe how you would prepare 250 ml of 0.2M phosphate buffer, pH =12.5, given solid Na2HPO4.2H2O and Na3PO4.H2O. pKa= 4.75
Throughout the table shown below, explain how you'll prepare a liter of phosphate buffer at a pH of 7.402 Phosphoric acid (H3PO4) Monosodium phosphate (NaH2PO4) Disodium phosphate (Na2HPO4) pKai = 2.16 pKaz = 7.21 pKaz = 12.32
answer question 3and 4
Question 2 (2 points) If 0.25 g of Na2HPO4 (disodium phosphate) is added to the 50 mL 0.30 M KH2PO4 solution, calculate the pH of the solution. Question 3 (2 points) Calculate the volume (in L) of 0.50 M NH4Cl to add to 50.00 ml of 1.0 M NH3 to preparea buffer at pH 9.00. Question 4 (2 points) Use the Henderson Hasselbach equation to calculate the pH of the solution which contains 10 mL of...
4. How much of (in mL) 0.15 M acetic acid (pKa 4.76) is needed to mix with 40 mL of 0.15 M sodium acetate to make a buffer of pH = 4.50 of 250 mL? (molar mass of acetic acid and sodium acetate are 60.0 g/mol and 82.0 g/ml, pKa of acetic acid is 4.76) (extra 10 pt)
3. How would you make 200 ml of a 250 mM phosphate buffer from a 1M stock solution of phosphate buffer? r. nus: how would you make 100 ul of 10 mM glucose solution from a 1M glucose stock solution? int: might need to do a serial dilution, as our pipettes cannot dispense less than 2ul)
(1) When calibrating a glass electrode, 0.025 M potassium dihydrogen phosphate/0.025 M disodium hydrogen phosphate buffer (pH = 6.881) gave a reading of -18.3 mV at 20 °C and 0.050 M potassium hydrogen phthalate buffer (pH = 3.788) gave a reading of +146.3 mV. What is the pH of an unknown giving a reading of +50.0 mV? What is the slope of the calibration curve (mV/pH units) and what is the theoretical slope at 20 °C? Find the value of...
Show calculations for the solutions.
Solutions for DNA cloning and DNA analysis a. 0.5M EDTA (disodium ethylenediaminetetra-acetate; tw 372.2), pH8.0 prepare 50mL hint: the disodium salt of EDTA will not go into solution until the pH of the solution is close to 8.0. To accomplish this, you will need to add approximately 2g of NaOH pellets b. 50XTAE (Tris-acetate/EDTA) (working solution at 1x is 0.04M Tris-acetate, 0.001M EDTA) Make 100mL 2M Tris base (fw 121.1) 5.71mL glacial acetic acid 0.05M...
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