(Western Blotting) What would happen if the blocking step were omitted? How would the blot look...
(Western Blotting)
What would happen if the blocking step were omitted? How would the blot look after the detection step?
Homework Answers
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(Western Blotting)
What would happen if the blocking step were omitted? How would
the blot look...
2) A blotting method known as Western blotting can be used to detect proteins that are...
2) A blotting method known as Western blotting can be used to detect proteins that are translated from a particular mRNA This techniques first separates proteins by size using electrophoresis then detects specific proteins through staining (antibody directed). Key is that you can visualize only the protein of interest on the gel. Suppose a researcher was interested in the effects of mutations on the expression of a gene that encodes protein X This protein is expressed in the skin and...
1. Western blotting: After western blotting process, the antibody detected too many non-specific bands, discuss possible...
1. Western blotting: After western blotting process, the antibody detected too many non-specific bands, discuss possible reasons and how to improve the specificity. 2.If there was not any band, discuss possible reasons and how to troubleshoot? 3. If cultured cells were overgrown (1:2 seeding ratio), how to perform subculture experiment differently? 4. Determine the confluence of each of seeding ratio by estimation (1:10 and 1:20 ratio) 5. Procedure in cell subculture?
Western Blotting Homework Assignment (1) (Protected View) - Word intain viruses. Unless you need to edit,...
Western Blotting Homework Assignment (1) (Protected View) - Word intain viruses. Unless you need to edit, it's safer to stay in Protected view Enable Editing Western Blotting Homework Assignment Either write your own one-page reflections of Western Blotting Te answer the following questions. 1. How western blot does works and explain some of its possible application? 2. While using the sample buffer (Leammli buffer), there was SDS (detergent). Why necessary for running the gel? 3. Why was it necessary for...
100 1. How western blot does works and explain some of its possible application? 2. While...
100 1. How western blot does works and explain some of its possible application? 2. While using the sample buffer (Leammli buffer), there was SDS (detergent). Why was the SDS necessary for running the gel? 3. Why was it necessary for the transfer to the nitrocellulose membrane? 4. The first stage of western blotting involves separating proteins using gel electrophoresis. On what basis does this technique separate proteins? 5. How can you test the efficiency of transfer of the protein...
this is western blotting experiment and I need your help with questions. I will send the...
this is western blotting experiment and I need your help with
questions. I will send the summary for this exiperiment
PRE-LAB PROTOCOL: Answer the following pre-lab questions: 1. 2. 4. How is mouse anti-goat lgG antibody generated? Why must sodium azide not be included in the secondary antibody incubation steps? Assuming that the overall yield of LDH enzyme in your prep was exactly 100%, how should the intensity of the LDH band in the NADH pool sample compare to that...
1) What does the appearance of a new band in a western blot mean? 2) When...
1) What does the appearance of a new band in a western blot mean? 2) When a band on a western blot increases in density over time, what does it mean? 3) When a band on a western blot decreases in density over time, what does it mean? 4) As it relates to EGF signaling, what process in the cell would possibly lead a band to increase in density over time? 5) As it relates to EGF signaling, what process...
Biotech Workshop HW 2 1. Compare and contrast Southern blot and Western blot on basic technique...
Biotech Workshop HW 2 1. Compare and contrast Southern blot and Western blot on basic technique such as procedure, reagents, its usage and applications. (5points) 2. Discuss common features of Random Probe Labeling share with RT-PCR. What are major differences between these two techniques? (5points) 3. It is claimed by a pharmaceutical company that protein A' is beneficial to treat breast cancer since it promotes apoptosis. If you were put into charge of testing this, how would you design experiment?...
Question 3 3 pts What makes GFP fusion proteins more useful than western blotting when studying...
Question 3 3 pts What makes GFP fusion proteins more useful than western blotting when studying expression of a specific gene during development? Select all correct. Western blotting requires you to kill the cells, but GFP fusions let you see the protein in living cells Western blotting lacks the spatial and temporal information that you can get GFP fusion proteins Western blotting detects mRNA, not protein. Western blotting amplifies the amount of total protein present, which prevents you from understanding...
Think about the two methods (the western and dot blot) how they differ, what unique benefits...
Think about the two methods (the western and dot blot) how they differ, what unique benefits might each technique present? When might one be more useful than the other?
What would be (what would it look like) if the the Linux ufw syntax were used...
What would be (what would it look like) if the the Linux ufw syntax were used for blocking the access of all outgoing traffic, except access to the cs.dal.ca website and SSH to the bluenose server?
2) A blotting method known as Western blotting can be used to detect proteins that are translated from a particular mRNA This techniques first separates proteins by size using electrophoresis then detects specific proteins through staining (antibody directed). Key is that you can visualize only the protein of interest on the gel. Suppose a researcher was interested in the effects of mutations on the expression of a gene that encodes protein X This protein is expressed in the skin and...
Western Blotting Homework Assignment (1) (Protected View) - Word intain viruses. Unless you need to edit, it's safer to stay in Protected view Enable Editing Western Blotting Homework Assignment Either write your own one-page reflections of Western Blotting Te answer the following questions. 1. How western blot does works and explain some of its possible application? 2. While using the sample buffer (Leammli buffer), there was SDS (detergent). Why necessary for running the gel? 3. Why was it necessary for...
100 1. How western blot does works and explain some of its possible application? 2. While using the sample buffer (Leammli buffer), there was SDS (detergent). Why was the SDS necessary for running the gel? 3. Why was it necessary for the transfer to the nitrocellulose membrane? 4. The first stage of western blotting involves separating proteins using gel electrophoresis. On what basis does this technique separate proteins? 5. How can you test the efficiency of transfer of the protein...
this is western blotting experiment and I need your help with
questions. I will send the summary for this exiperiment
PRE-LAB PROTOCOL: Answer the following pre-lab questions: 1. 2. 4. How is mouse anti-goat lgG antibody generated? Why must sodium azide not be included in the secondary antibody incubation steps? Assuming that the overall yield of LDH enzyme in your prep was exactly 100%, how should the intensity of the LDH band in the NADH pool sample compare to that...
Biotech Workshop HW 2 1. Compare and contrast Southern blot and Western blot on basic technique such as procedure, reagents, its usage and applications. (5points) 2. Discuss common features of Random Probe Labeling share with RT-PCR. What are major differences between these two techniques? (5points) 3. It is claimed by a pharmaceutical company that protein A' is beneficial to treat breast cancer since it promotes apoptosis. If you were put into charge of testing this, how would you design experiment?...
Question 3 3 pts What makes GFP fusion proteins more useful than western blotting when studying expression of a specific gene during development? Select all correct. Western blotting requires you to kill the cells, but GFP fusions let you see the protein in living cells Western blotting lacks the spatial and temporal information that you can get GFP fusion proteins Western blotting detects mRNA, not protein. Western blotting amplifies the amount of total protein present, which prevents you from understanding...
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