Design an optimization experiment to determine the optimum pH for growth of E. coli that produce...
Design an optimization experiment to determine the optimum pH for growth of E. coli that produce GFP.
Homework Answers
Ideal ph for the growth of E.coli is around 7.
As per the growth curve of bacteria, a good culture of bacteria is the one in which number of cells in log phase is the maximum. This is determined by taking the OD (optical density) at different conditions. Ideally, this OD at 600 nm should be around 0.2 to 0.3.
So, to determine the ph for this E.coli that produces GFP, incubate the given cells overnight, in a new culture medium at ph 7. Check their OD after overnight incubation. Take this reading for 5 to 6 times after 15-20 minutes interval.
Now, repeat the same experiment by decreasing and increasing the ph of culture medium. The optimum ph will be the one in which you find the density of bacterial cells maximum at log phase.
If you find the answer useful, then please hit the like button.
Add Answer to:
Design an optimization experiment to determine the optimum pH
for growth of E. coli that produce...
Purification of Recombinant GFP from E. coli Was the GFP present in the supernatant or the...
Purification of Recombinant GFP from E. coli Was the GFP present in the supernatant or the pellet of cell debris after the last centrifugation step of the E.Coli lysis experiment? Does this differential localization indicate that GFP is a globular or a fibrous/membrane type protein? Explain your reasoning.
1. In a transformation experiment, E. coli uptake pRGLO plasmids that carry GFP gene. The transformed...
1. In a transformation experiment, E. coli uptake pRGLO plasmids that carry GFP gene. The transformed bacterial cells are grown on an ampicillin-treated agar plate. Which of the following is considered as a vector? GFP gene C E. coli cell c ampicillin-treated agar plate 0.5 points QUESTION 2 1. What is the function of bla gene? Break down the ampicillin Regulate the transcription of GFP gene Express the fluorescent trait in bacteria Control the uptake of DNA molecules in bacteria...
Explain how you might use E. coli bacteria to produce human growth hormone using the following:...
Explain how you might use E. coli bacteria to produce human growth hormone using the following: plasmid, b. Gene segment for human growth hormone that contains only exons, DNA ligase, Restriction enzymes, and Incubator for growing bacteria cells.
Only information given Explain how you might engineer E. coli to produce human growth hormone (HGH)...
Only information given
Explain how you might engineer E. coli to produce human growth hormone (HGH) using the following. E. coli containing a plasmid DNA carrying the gene for HGH DNAIigase A restriction enzyme Equipment for manipulating and growing bacteria A method for extracting and purifying the hormone An appropriate DNA probe For this problem, assume the HGH gene lacks introns.
To study the lac operon in E. coli, you added IPTG in the growth media, which...
To study the lac operon in E. coli, you added IPTG in the growth media, which is another analog of allolactose that is an inducer of the lac operon but is not metabolized by β-gal. You measured the activity of the enzyme β- galactosidase (β-gal) by X-gal. After mutagenesis (induce mutations) you find mutants that never turn blue, which DNA component(s) could produce such phenotype?
E. Coli has a doubling time of 20 minutes under certain growth conditions. Compute the biomass...
E. Coli has a doubling time of 20 minutes under certain growth conditions. Compute the biomass of E. coli (in pounds) that will accumulate when a single cell of E. coli grows exponentially for 50 hours in these conditions, assuming that nutrients are not limiting. Show your work. (An E. Coli cell has a mass of 10-12gram/cell).
What would be some reasons as to why a bacterial growth curve would produce unexpected results?...
What would be some reasons as to why a bacterial growth curve
would produce unexpected results? For example, an E. coli culture
increases after the stationary phase? Or why there is no
stationary phase? I am trying to
interpret results that I have from a lab experiment.
Viable Cell Concentration vs. Time of Escherichia coli E 1.00E+08 1.00E-07 C 1.00E+06 60 80 100 120 140 0 20 40 Time (minutes) Spectrophotometer -96 Well Plate
E. coli and E. aerogenes Data Shee MRVP Some lab instructions call for a shorter incubation...
E. coli and E. aerogenes
Data Shee MRVP Some lab instructions call for a shorter incubation time, while other protocols call for a longer time. Which of the two tests would likely produce more false negatives with a shorter incubation Which test would benefit most from a longer incubation time? What organism does not perform mixed acid fermentation? What organism does not perform 2,3 butanediol fermentation? Name the substrate for the MRVP. Name the products and their pH for the...
Facultative anaerobes such as E. coli Group of answer choices require O2 for growth require the...
Facultative anaerobes such as E. coli Group of answer choices require O2 for growth require the absence of O2 for growth do not require O2, but use it when it is available do not require or use O2, but can tolerate it
1. In a phosphate limitation environment, E. coli will be induced to produce alkaline phosphatase. (A)...
1. In a phosphate limitation environment, E. coli will be induced to produce alkaline phosphatase. (A) What is the function of alkaline phosphatase? And how does it help the bacteria to survive in this environment? (B) The phosphate sensing system of E. coli is an example of sensor kinase system. Describe the molecular mechanism of the phosphate sensing system of E. coli using the sensor kinase model. 2. Pseudomonas aeruginosa is used as a bacterial model to study biofilm mechanism....
1. In a transformation experiment, E. coli uptake pRGLO plasmids that carry GFP gene. The transformed bacterial cells are grown on an ampicillin-treated agar plate. Which of the following is considered as a vector? GFP gene C E. coli cell c ampicillin-treated agar plate 0.5 points QUESTION 2 1. What is the function of bla gene? Break down the ampicillin Regulate the transcription of GFP gene Express the fluorescent trait in bacteria Control the uptake of DNA molecules in bacteria...
Only information given
Explain how you might engineer E. coli to produce human growth hormone (HGH) using the following. E. coli containing a plasmid DNA carrying the gene for HGH DNAIigase A restriction enzyme Equipment for manipulating and growing bacteria A method for extracting and purifying the hormone An appropriate DNA probe For this problem, assume the HGH gene lacks introns.
What would be some reasons as to why a bacterial growth curve
would produce unexpected results? For example, an E. coli culture
increases after the stationary phase? Or why there is no
stationary phase? I am trying to
interpret results that I have from a lab experiment.
Viable Cell Concentration vs. Time of Escherichia coli E 1.00E+08 1.00E-07 C 1.00E+06 60 80 100 120 140 0 20 40 Time (minutes) Spectrophotometer -96 Well Plate
E. coli and E. aerogenes
Data Shee MRVP Some lab instructions call for a shorter incubation time, while other protocols call for a longer time. Which of the two tests would likely produce more false negatives with a shorter incubation Which test would benefit most from a longer incubation time? What organism does not perform mixed acid fermentation? What organism does not perform 2,3 butanediol fermentation? Name the substrate for the MRVP. Name the products and their pH for the...
Most questions answered within 3 hours.
-
Say true or false and explain it.
A heap is represented using an array. The array...
asked 6 minutes ago -
Explain how the DNA replication enzyme complex is organized and
functions, please don't copy paste from...
asked 6 minutes ago -
how
can personality contribute to one scoring high on the need to
evaluate measurement by Jarvis?
asked 7 minutes ago -
A high jumper is attempting to jump over a crossbar that is
placed at a height...
asked 11 minutes ago -
1.
A hemophiliac male mates with a healthy female. what are the
possible genotypes for the...
asked 28 minutes ago -
You could argue that even though Rockefeller clearly had
enormous market power (90% of the market)...
asked 43 minutes ago -
At the beginning of the year, Palermo Brothers, Inc., purchased
a new plastic water bottle making...
asked 43 minutes ago -
Calculate the change in the Gibbs energy of the system for the
following processes: a) 6.0...
asked 32 minutes ago -
After reading the Primary Care scenario in the Allied Health
Community, what could have been implemented...
asked 37 minutes ago -
A solution is prepared by dissolving 11.60 g of a mixture of
sodium carbonate and sodium...
asked 41 minutes ago -
1. Mini Case Study: Suppose that, in the future, most jobs will
be bid upon by...
asked 44 minutes ago -
Plantinga argues for the view that there is no solution to the
problem of moral evil....
asked 39 minutes ago