6. Draw and label a typical gene including the following:
a. 5’ and 3’ ends
b. core and proximal promoters
c. protein coding region
d. 5’ and 3’ UTR
e. 2 Introns and 3 exons
Non coding DNA is found within most eukaryotic genes. such geens
have a split struture in which segments of coding sequence (exons)
are seperated by non coding sequences(introns) the entire gene is
trancribed to yield a long RNA molecule and the introns are then
removed by splicing, so only exons are included in the mRNA. The
process of excising the sequences in RNA that correspond to introns
and joining of sequences corresponding to the exons is called RNA
splicing.
6. Draw and label a typical gene including the following: a. 5’ and 3’ ends b....
(Molecular Biology) Draw a diagram of a gene region in DNA from the promoter to end of the 3' UTR. Label the TATA box and BRE, the transcription start site, translation start site, 5' UTR, any at least two exons and 1 intron, 3' UTR, and start and stop codons.
Draw a picture of a standard gene and label 3 kinds of cis-factors (core, proximal, distal) and 2 kinds of trans-factors (general and regulatory).
Draw a typical prokaryotic gene with its promoter and terminator. Draw the primary transcript Label (using letters) all the parts (including sites of consensus sequences) in both the gene and the mRNA. Indicate what the ‘letters stand for. (Use the line drawing below). What proteins are involved in transcription initiation, elongation and termination in prokaryotic genes. +1 5’ ________________________________________________________________ 3’ 3’ ________________________________________________________________ 5’
3. Prokaryotic and eukaryotic gene expression compared. Below is an incomplete table of prokaryotic and eukaryotic gene expression in comparison. Fill in the blank using PPT slides, notes and the textbook. Prokaryotic gene expression Eukaryotic gene expression Overview Steps Transcription and translation Yes Transcription and translation coupled? Gene structure No introns Epigenetic modification (chromosome remodeling) transcription, translation, RNA processing, protein processing Transcription in the nucleus and translation in the cytoplasm Interrupted gene with exons and introns RNAPI, II, III Which...
QUESTION 6 Assume you are studying a protein-coding gene, ACEX, which includes 4 exons as illustrated in the gene map below. The 5' UTR and 3' UTR segments are each 25 bp long. Exons 1 thru 4 are 100, 200, 300, 400 bp long, respectively. Each intron is 200 bp each. The locations of the relevant EcoRI sites within the ACEX locus are indicated, but the location of other restriction enzyme sites (like BamHI) are not shown." EcoRI probe EcoRI...
1) Suppose that gene A 3,000 bp. Suppose that g contained within intron 1 opposite directions for the two genes. covers 10,000 base pairs (bp) and has 2 exons; the intron in gene A is ene B covers 1,500 bp and has two exons. Gene B is completely of gene A. The direction of the transcriptional bubble moves in A. Draw the genomic organization (i.e., exons and introns) of gene A AND gene B. Label the polarity of the DNA...
A gene has 3 exons and 2 introns. While analyzing a mutant, you discover that 9 basepairs are inserted in the middle of intron 1 of this gene. What is the effect of this insertion on the protein? Select one: a. this insertion will have no effect on the protein b. The protein is likely non-functional because the insertion caused a frameshift c. The protein has 3 extra amino acids d. the transcription will be terminated e. the type of...
Expert Q&A Done 1. Is this strand the sense strand or anti-sense strand? What is your evidence (How did you know)? So is this the template or coding strand? 2. How would you locate the general area of the promoter? Hint: what sequence would you look for? Underline the sequence and label with an (a). What sequence would you look for if the complementary strand was provided? (Always write answers in 5 to 3" unless otherwise 3. How many exons...
Please answer 4 and 5?
4. Alpha amanitin is found in poisonous mushrooms and irreversibly binds RNA polymerase lI in eukaryotic cells. Why do people die when they eat poisonous mushrooms? (Stating they are poisonous is not an acceptable answer. You need to explain why.] 13 points] 5. Draw a diagram representing the structure of the human DABEARS gene. This gene has 4 exons. The complete precursor mRNA sequence is 2017 bp, [6 pts] a. First draw the gene indicating:...
3of 3 9. The figure below represents the primary transcript of a gene that contains four exons (A, B, C, D) and two introns. The dark block in exon B indicates the position of an additional stop codon; the normal start and stop codons for translation are present in exons A and D respectively. The two arrows indicate alternative 3' splice sites for the first intron Pre-mRNA 5'I 3' intron intron Give a schematic representation of the mature mRNAs that...