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how do you write an instruction manual for a bacterial transformation kit?
What happens to the transformed bacterial colonies and effects on the transformation efficiency if the recovery phase is left out while doing the pGLO transformation of E.coli bacteria? How does the amount of DNA used in the transformation reaction affect the transformation efficiency?
You calculated the gene density for the chromosomes of the bacterial species E.coli, the fruit fly Drosophila, chickens and humans. Which species had the most genes/Mb DNA? Humans b. Drosophila 6. Chickens d. E.coli
answer Question 16&17 > Question 16 2 In the transformation lab, the transformed E.coli O produced arabinose sugars were killed by ampicillin o survived ampicillin Question 17 2 pts Bacterial plasmids are the only genetic materials in bacteria. True or False? (If any portion of the statement is false, the entire statement is false). False True
10) Define bacterial transformation? How did Frederick Griffith demonstrate that bacterial strains could be genetically transformed?
How do you write a lab report on Identification of unknown bacteria with the results being 1. E.coli and 2. S.a ?
During a particular transformation protocol, you used a stock of PGLO DNA with a concentration of 0.08 ug ul. You transformed 191 UL of competent E. coli with 100L of PGLO DNA. You added 191 UL of LB broth to the transformation before plating 100 uL of the culture to a plate labeled +POLO, LB, Amp, Ar. After growing the plates overnight, 136 fluorescent colonies (transformants) were counted. What was the average transformation efficiency of your work?
Suppose that you carried out a Bacterial transformation of E. coli HA101 with pGLO plasmid experiment in the lab. During the experiment plates with bacteria were inoculated from +GLO and -GLO microfuge tubes (LB (-) plate, LB/amp (-) plate, LB/amp (+) plate, and LB/amp/ara (+) plate). 14) Explain what kinds of bacterial growth you will find on each of 4 plates after incubation ((LB (-) plate, LB/amp (-) plate, LB/amp (+) plate, and LB/amp/ara (+) plate): under normal light and...
how do you estimate the download protocol overhead or percentage of the download bytes taken up by protocol overhead on wireshark
You are doing a bacterial transformation in Professor Carrico’s Genetics lab. As you are getting ready, you realize that you are out of a critical solution, Calcium Chloride. You look at the bottle and find the Molecular Weight CaCl2 is listed as 110.98g/mol. You need 10 ml of a 2 molar solution. Briefly describe how you would proceed to make this solution.