Describe three methods (other than plaque counting) to determine virus concentration? (referencing required)

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Answer 1

Answer #1

Answer: These are the techniques which can be used to determine virus concentration:

Technique	Detection Principle	Reproducibility	Time	Labour	Cost
immunofluorescence foci assays	infectivity assay	poor	days	high	expensive
qPCR	viral nucleic acid	excellent	hours	moderate	expensive
immunoblotting	viral protein	good	days	moderate	inexpensive
immunoprecipitation	viral protein	good	days	moderate	inexpensive
ELISA	viral protein	good	hours	moderate	inexpensive
hemagglutination assay	viral protein	good	hours	moderate	inexpensive
viral flow cytometry	viral particle	excellent	hours	high	expensive
transmission electron microscopy	viral particle	excellent	weeks	high	expensive

Reference:

Answer 2

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