PCR is an in vitro technique that can be said to mimic a biological function in...
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PCR is an in vitro technique that can be said to mimic a biological function in any living cell.
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a) What is this biological function?
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b) Give two similarities in functions between a PCR reaction and you answer in (a) above
Homework Answers
a) Answer- PCR (polymerase chain reaction) mimics the biological DNA replication process where in biological replication process DNA polymerase synthesizes inside cell and in PCR DNA is synthesized in tet tube in many cycles to produces many copies of DNA.
b) Answer- similarities between cellular DNA replication and PCR are -
1. In cellular DNA replication and in PCR, DNA strand serve as template and free nucleotides are required to elongate new DNA strand.
2. The direction of DNA synthesis is 5' to 3' in in vivo and in vitro DNA replication because the DNA polymerase has 5' to 3' polymerase activity.
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PCR is an in vitro technique that can be said to mimic a
biological function in...
Consider a standard PCR reaction. If instead of including double-stranded DNA from a biological sample, you ...
Consider a standard PCR reaction. If instead of including double-stranded DNA from a biological sample, you added mRNA molecules from a biological sample, the reaction would… Can you please explain why the answer is not E A. work, but would yield less product because you started off with single-stranded template B. A and D are correct C. work, but would generate much shorter products D. not work because you would also need to replace the dNTPs with NTPs for the...
Identification of unknown Bacteria by sequencing rDNA Having a little trouble understanding the PCR process. This...
Identification of unknown Bacteria by sequencing rDNA Having a little trouble understanding the PCR process. This is a lab we did and some homework questions regarding the sequencing rDNA to find unknown bacteria. I hope by answering these I can have better understanding of the process. The more descriptive the better. Thank you! 2. A. List the reagents used in preparing master mix for PCR and write one sentence about why each one was necessary. B. Now that you have...
Person A said that given a transfer function H(s) the system was unique as the impulse...
Person A said that given a transfer function H(s) the system was
unique as the impulse function h(t) is fixed. Person B
said that two different systems can have the same transfer
function. Then he produced the following two ODEs:
(a) System A that is given by dx dt dt2 dt (b) System B that is given by dy dt Can you verify that the transfer functions for the two systems are the same but prove that the systems are...
This is also for the Unknown 3 I am doing for microbiology. If you could please...
This is also for the Unknown 3 I am doing for microbiology. If you could please help in detail thanks. A. Now that you have confirmed that PCR product is of correct size, using agarose gel electrophoresis, how will you find out that the amplified DNA fragment is our desired target region? B. What is the principle of Sangers’ sequencing technique? Use a labeled diagram (may copy & paste image from internet) to briefly describe the technique. Write two differences...
1. Fill out the following table by indicating which general technique (light microscopy (LM) or electron...
1. Fill out the following table by indicating which general technique (light microscopy (LM) or electron microscopy [EM]) could be used to observe each structure or phenomenon. Put "no" in the box if the technique could not be used. If light microscopy can be used, name one technique (bright-field, phase-contrast, fluorescence, etc.) that you think would be effective. You will find some useful information in Appendix 1 of this manual and Chapter 18 of your textbook. Structure or phenomenon Could...
Plz use MGF technique The lifetime of an electronic component in an HDTV is a random...
Plz use MGF technique
The lifetime of an electronic component in an HDTV is a random variable that can be modeled by the exponential distribution with a mean lifetime ß. Two components, X1 and X2, are randomly chosen and operated until failure. At that point, the lifetime of each component is observed. The mean lifetime of these two components is X1 + X2 X =- a) Find the probability density function of x using the MGF technique (the method of...
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Question 1 Can the biological species concept be applied to bacterial species M. bovis and M. tuberculosis? I think the answer is (A) but would appreciate confirmation and reasoning. A) No, M. bovis and M. tuberculosis don't reproduce sexually so species can't be defined based on the ability to interbreed. B) Yes, they are living, biological, organisms so the biological species concept is applicable. C) No, M. bovis and M. tuberculosis inhabit different hosts so don't interbreed in nature. Question...
4. (1.5 pts)You are practicing designing primers that you can use in PCR reactions. You want...
4. (1.5 pts)You are practicing designing primers that you can use in PCR reactions. You want your primers to allow you to amplify the sequence found below. 5°-АсттсслтАTстсТАЛААТАССАТCGATСтстсссссстАGстAСCТААССАGAGACCСТАССG-3. 3-тслАсстатАсAGATTTTATсстасстаGлCAссссССATCCATCGAтTСстстстасGAТСCС-5. Markers part (a) part (b) part ck Right primer should anneal to this region Left primer should 87 nts anneal to this region 80 nts 70 nts 60 nts 50 nts 40 nts 27 nts Draw into the following gel lanes what size(s) of PCR products you would get if you...
8. PCR is used to. A Diagnose genetic disease 8 Solve cnmes C Sudy gene unction...
8. PCR is used to. A Diagnose genetic disease 8 Solve cnmes C Sudy gene unction D. All of th C ONA as a template to form RINA D All of the above 7. PCR technique does not need A. Tag polymerase B Restriion encymes C Olgoucletide prmers C. A fragment of skin D. All of the above 9 PCR can be used in A Cloning B.Sequening C.Medical dagnosis&foric mine 0.PCR can make mullple copies ot A. DNA B RNA...
Multiple Choice Which of the following is not a data validation technique? (a) The Round function...
Multiple Choice Which of the following is not a data validation technique? (a) The Round function (b) Lookup fields (c) Default values (d) Input masks 6. When would you use the Date function (a) To display the current date (b) To display only the year from a date field (c) When doing math involving two date fields (d) To convert a value from a number into a date 2. Which of the following fields is least likely to be set...
Person A said that given a transfer function H(s) the system was
unique as the impulse function h(t) is fixed. Person B
said that two different systems can have the same transfer
function. Then he produced the following two ODEs:
(a) System A that is given by dx dt dt2 dt (b) System B that is given by dy dt Can you verify that the transfer functions for the two systems are the same but prove that the systems are...
1. Fill out the following table by indicating which general technique (light microscopy (LM) or electron microscopy [EM]) could be used to observe each structure or phenomenon. Put "no" in the box if the technique could not be used. If light microscopy can be used, name one technique (bright-field, phase-contrast, fluorescence, etc.) that you think would be effective. You will find some useful information in Appendix 1 of this manual and Chapter 18 of your textbook. Structure or phenomenon Could...
Plz use MGF technique
The lifetime of an electronic component in an HDTV is a random variable that can be modeled by the exponential distribution with a mean lifetime ß. Two components, X1 and X2, are randomly chosen and operated until failure. At that point, the lifetime of each component is observed. The mean lifetime of these two components is X1 + X2 X =- a) Find the probability density function of x using the MGF technique (the method of...
4. (1.5 pts)You are practicing designing primers that you can use in PCR reactions. You want your primers to allow you to amplify the sequence found below. 5°-АсттсслтАTстсТАЛААТАССАТCGATСтстсссссстАGстAСCТААССАGAGACCСТАССG-3. 3-тслАсстатАсAGATTTTATсстасстаGлCAссссССATCCATCGAтTСстстстасGAТСCС-5. Markers part (a) part (b) part ck Right primer should anneal to this region Left primer should 87 nts anneal to this region 80 nts 70 nts 60 nts 50 nts 40 nts 27 nts Draw into the following gel lanes what size(s) of PCR products you would get if you...
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Multiple Choice Which of the following is not a data validation technique? (a) The Round function (b) Lookup fields (c) Default values (d) Input masks 6. When would you use the Date function (a) To display the current date (b) To display only the year from a date field (c) When doing math involving two date fields (d) To convert a value from a number into a date 2. Which of the following fields is least likely to be set...
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