You have an assay that has a range of 20-500 ug in a 500 ul sample....
You have an assay that has a range of 20-500 ug in a 500 ul sample. What is the concentration range for the samples used in the standard curve? You have one sample that you wish to run to determine the molecular content and have approximately 500 mg in two ml. How will you dilute (or not) to do this assay?
Homework Answers
The concentration range is 40 ug/ml to 1 mg/ml of the standard Curve.
The given strength of the solution is 500 mg/2 ml, which is 250 mg/ml. I will be diluting the solution atleast 1000 fold to get the concentration to 250 ug/ml and well within the range of Standard Curve.
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You have an assay that has a range of 20-500 ug in a 500 ul
sample....
Question 14 4 pts A protein assay was performed using the Bradford Reagent to determine the...
Question 14 4 pts A protein assay was performed using the Bradford Reagent to determine the protein concentrations of a series of pet food extract samples. The BSA Standard Curve had a slope of 0.0173 Data obtained for the pet food are shown in the table below. Tubes 1-2 contained different volumes of the pet food extract. Tube # Volume Sample (UL) A(595) Volume Volume Water Reagent (3.0 (L) mL) 803 503 1 2 20 50 0.293 0.657 What is...
you have a solution containing 9 ug/ul of DNA. How would you dilute this to 1...
you have a solution containing 9 ug/ul of DNA. How would you dilute this to 1 ug/ul? make your final volume 0.1 ml
concentration of the stock is 39.2mg 3. (6 pts) Figure out your plan for making each...
concentration of the stock is 39.2mg
3. (6 pts) Figure out your plan for making each solution required in the protocol. Be certain you FINISH with the required volumes of EACH solution! a. Calculate what is needed to make the 4 mg/ml solution b. Calculate what is needed to make the 400 ug/ml working solution c. Calculate what is needed to make the 40 ug/ml working solution You are provided with the following portion of a protocol: Determine concentration of...
Please answer #2. I'm unsure how its done ete od SOCK Solutions (the concentrations are known...
Please answer #2. I'm unsure how its done
ete od SOCK Solutions (the concentrations are known you prepare will be used in assessing concent (0.07wv) to be used in developing a spectrophotometric assay for this drug. The standard curve your disposal a primary standard solution of the drug in sal w be used in assessing concentrations of the drug in samples having unknown concentrations. You have at this STD). The linear range of the assay for this drug a primary...
you assay an unknown sample and its concentration falls below the linear range of your standard...
you assay an unknown sample and its concentration falls below the linear range of your standard curve. what can you do to solve this problem?
a) You want to load 15 ug of protein in 10 uL into one of the...
a) You want to load 15 ug of protein in 10 uL into one of the 12% polyacrylamide gel well. The protein needs to be in 1X buffer and in a total volume of 0.100 ml. You are given a 4.5 mg/ml protein solution, a 20% sample buffer, and distilled water. How much of each would you mix together to make required volume? (5 pts)
1. You have generated the following standard curve after a Bradford Assay to measure Absorbance vs...
1. You have generated the following standard curve after a Bradford Assay to measure Absorbance vs samples of known BSA concentrations. Now you need to determine the amount of protein in two mitochondria samples taken from fish, one taken from fish under normal conditions and the other taken from fish under oxidative stress. The absorbance reading for the normal fish fraction was A595=175, and the absorbance reading for the stressed fish fraction was A595= 275 Bradford Assay Standard Curve y...
The linear concentration range for the Bradford assay is 20-2000 µg/mL of protein. Protein concentrations below...
The linear concentration range for the Bradford assay is 20-2000 µg/mL of protein. Protein concentrations below or above these ranges cannot be measured accurately. If it is suspected that a sample contains greater than 50 mg/mL of protein, how can the protein concentration still be measured using the Bradford assay? Explain.
2. You are performing an assay to measure the TNF-a concentration in some patient serum sample....
2. You are performing an assay to measure the TNF-a concentration in some patient serum sample. To perform the assay, you need a set of standards of known TNF-a concentration. How would you prepare TNF-a standards of 10, 50, 100, 250, 500 and 1,000 pg/ml from a 10 ng/ml TNF-a stock by serial dilution? [you need at least 200 ul of each for to perform the assay]
You have 0.5 mL sample of protein extract (absorbance 0.5930) with a concentration of 181.2 ug/mL....
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Question 14 4 pts A protein assay was performed using the Bradford Reagent to determine the protein concentrations of a series of pet food extract samples. The BSA Standard Curve had a slope of 0.0173 Data obtained for the pet food are shown in the table below. Tubes 1-2 contained different volumes of the pet food extract. Tube # Volume Sample (UL) A(595) Volume Volume Water Reagent (3.0 (L) mL) 803 503 1 2 20 50 0.293 0.657 What is...
concentration of the stock is 39.2mg
3. (6 pts) Figure out your plan for making each solution required in the protocol. Be certain you FINISH with the required volumes of EACH solution! a. Calculate what is needed to make the 4 mg/ml solution b. Calculate what is needed to make the 400 ug/ml working solution c. Calculate what is needed to make the 40 ug/ml working solution You are provided with the following portion of a protocol: Determine concentration of...
Please answer #2. I'm unsure how its done
ete od SOCK Solutions (the concentrations are known you prepare will be used in assessing concent (0.07wv) to be used in developing a spectrophotometric assay for this drug. The standard curve your disposal a primary standard solution of the drug in sal w be used in assessing concentrations of the drug in samples having unknown concentrations. You have at this STD). The linear range of the assay for this drug a primary...
a) You want to load 15 ug of protein in 10 uL into one of the 12% polyacrylamide gel well. The protein needs to be in 1X buffer and in a total volume of 0.100 ml. You are given a 4.5 mg/ml protein solution, a 20% sample buffer, and distilled water. How much of each would you mix together to make required volume? (5 pts)
1. You have generated the following standard curve after a Bradford Assay to measure Absorbance vs samples of known BSA concentrations. Now you need to determine the amount of protein in two mitochondria samples taken from fish, one taken from fish under normal conditions and the other taken from fish under oxidative stress. The absorbance reading for the normal fish fraction was A595=175, and the absorbance reading for the stressed fish fraction was A595= 275 Bradford Assay Standard Curve y...
2. You are performing an assay to measure the TNF-a concentration in some patient serum sample. To perform the assay, you need a set of standards of known TNF-a concentration. How would you prepare TNF-a standards of 10, 50, 100, 250, 500 and 1,000 pg/ml from a 10 ng/ml TNF-a stock by serial dilution? [you need at least 200 ul of each for to perform the assay]
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