you assay an unknown sample and its concentration falls below the linear range of your standard...
you assay an unknown sample and its concentration falls below the linear range of your standard curve. what can you do to solve this problem?
Homework Answers
If the sample concentration falls below the linear range, the dilution of the sample should be reduced. The standard curve can also be done with the new dilutions along with the sample.
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you assay an unknown sample and its concentration falls below
the linear range of your standard...
You have an assay that has a range of 20-500 ug in a 500 ul sample....
You have an assay that has a range of 20-500 ug in a 500 ul sample. What is the concentration range for the samples used in the standard curve? You have one sample that you wish to run to determine the molecular content and have approximately 500 mg in two ml. How will you dilute (or not) to do this assay?
The linear concentration range for the Bradford assay is 20-2000 µg/mL of protein. Protein concentrations below...
The linear concentration range for the Bradford assay is 20-2000 µg/mL of protein. Protein concentrations below or above these ranges cannot be measured accurately. If it is suspected that a sample contains greater than 50 mg/mL of protein, how can the protein concentration still be measured using the Bradford assay? Explain.
In biochemistry, we use a 'Bradford Assay' to calculate the concentration of protein in a sample....
In biochemistry, we use a 'Bradford Assay' to calculate the concentration of protein in a sample. This method uses the same principles of the standard curve from this experiment. In a Bradford Assay, a standard curve is prepared using a known protein, typically Bovine Serum Albumin (BSA), but rather than measuring the density, we can measure a different property - its 'absorbance'. The graph below is representative of a standard curve from a Bradford Assay (with the line equation displayed...
They performed a BSA assay to determine the concentration of an unknown solution X. The graph...
They performed a BSA assay to determine the concentration of an unknown solution X. The graph below is the BSA standard curve. In the cuvette, you add 25 ul of the unknown solution X, 200 ul of the Bradford’s reagent and 775 ul dH2O. You obtain an absorbance of 0.58 at 595nm. Use all the information to calculate the concentration of the unknown solution X. Show work Y- 0.0457x R^2- 0.9901
A Bradford assay was carried out to measure protein concentration. The absorbance of five standard samples...
A Bradford assay was carried out to measure protein concentration. The absorbance of five standard samples and two unknown samples are listed in the table below. The Bradford reaction mixture (1 ml) was prepared as follows: 100 μl sample A or sample B + 200 μl Bradford reagent + 700μl 0.9% NaCl solution sample ID concentration (mM) absorbance (O.D.) std 1 100 0.78 std 2 50 0.4 std 3 25 0.18 std 4 12.5 0.08 std 5 0 0 ...
1. You have generated the following standard curve after a Bradford Assay to measure Absorbance vs...
1. You have generated the following standard curve after a Bradford Assay to measure Absorbance vs samples of known BSA concentrations. Now you need to determine the amount of protein in two mitochondria samples taken from fish, one taken from fish under normal conditions and the other taken from fish under oxidative stress. The absorbance reading for the normal fish fraction was A595=175, and the absorbance reading for the stressed fish fraction was A595= 275 Bradford Assay Standard Curve y...
3. Suppose that an unknown sample is analyzed spectrophotometrically at the same wavelength that was used...
3. Suppose that an unknown sample is analyzed
spectrophotometrically at the same wavelength that was used for the
standard curve and the absorbance was found to be 0.196. Use the
standard curve to find the concentration of the unknown.
1. Calculate the concentration of a solution prepared by adding 15.00 mL of 1.98 x 10-3 M KMnO, from a buret into a 50.00 mL volumetric flask, which is then filled to the 50.00 mL graduation mark with distilled water. Hint:...
2. A linear standard curve of KMnO is prepared from a set of standard solutions by...
2. A linear standard curve of KMnO is prepared from a set of standard solutions by plotting absorbance (y) vs. concentration (x). Use Logger Pro (as described in the Data Analysis for this experiment), or Google Sheets/Excel, to plot this standard curve using the data in Table 1. Help with spreadsheets [PDF] Table 1: Data for the standard curve of KMnO4 Absorbance Concentration (M) 0.330 6.00 x 10-4 0.264 4.80 x 10-4 0.198 3.60 x 10-4 0.132 2.40 x 10-4...
thank you a) Do a Michaelis-Menton plot of the enzyme assay data below. Substrate Concentration mg/mL...
thank you
a) Do a Michaelis-Menton plot of the enzyme assay data below. Substrate Concentration mg/mL Velocity umole/(min-mL) 10 15 20 31 50 75 100 125 250 330 380 437 524 612 662 678 696 713 Have the substrate concentration as the x-axis of the graph, and the velocity as the y-axis. . Use a grid line every 10 substrate concentration units, and a grid line every 20 velocity units. . In Excel, gridlines can be controlled under the Chart...
In my lab, we ran a BCA assay for protein concentration and generated this standard curve,...
In my lab, we ran a BCA assay for protein concentration and
generated this standard curve, but I have absolutely no idea what
this chart is telling me. Please explain if you can! Thank you.
Curve 3.000 2.500+ 2.000 562 1.500+ 1.000 0.500 0.000+ -500 500 1500 2000 2500 1000 <Plate Layout Settings R2 Curve Curve Name Formula Curve Y=A*X+B Curve Curve Paramet Name Formula er Curve Y=A*X+B A B Fit F Prob 0.00101 0.2020.995 ????? Std. 95% CI 95%...
In biochemistry, we use a 'Bradford Assay' to calculate the concentration of protein in a sample. This method uses the same principles of the standard curve from this experiment. In a Bradford Assay, a standard curve is prepared using a known protein, typically Bovine Serum Albumin (BSA), but rather than measuring the density, we can measure a different property - its 'absorbance'. The graph below is representative of a standard curve from a Bradford Assay (with the line equation displayed...
1. You have generated the following standard curve after a Bradford Assay to measure Absorbance vs samples of known BSA concentrations. Now you need to determine the amount of protein in two mitochondria samples taken from fish, one taken from fish under normal conditions and the other taken from fish under oxidative stress. The absorbance reading for the normal fish fraction was A595=175, and the absorbance reading for the stressed fish fraction was A595= 275 Bradford Assay Standard Curve y...
3. Suppose that an unknown sample is analyzed
spectrophotometrically at the same wavelength that was used for the
standard curve and the absorbance was found to be 0.196. Use the
standard curve to find the concentration of the unknown.
1. Calculate the concentration of a solution prepared by adding 15.00 mL of 1.98 x 10-3 M KMnO, from a buret into a 50.00 mL volumetric flask, which is then filled to the 50.00 mL graduation mark with distilled water. Hint:...
2. A linear standard curve of KMnO is prepared from a set of standard solutions by plotting absorbance (y) vs. concentration (x). Use Logger Pro (as described in the Data Analysis for this experiment), or Google Sheets/Excel, to plot this standard curve using the data in Table 1. Help with spreadsheets [PDF] Table 1: Data for the standard curve of KMnO4 Absorbance Concentration (M) 0.330 6.00 x 10-4 0.264 4.80 x 10-4 0.198 3.60 x 10-4 0.132 2.40 x 10-4...
thank you
a) Do a Michaelis-Menton plot of the enzyme assay data below. Substrate Concentration mg/mL Velocity umole/(min-mL) 10 15 20 31 50 75 100 125 250 330 380 437 524 612 662 678 696 713 Have the substrate concentration as the x-axis of the graph, and the velocity as the y-axis. . Use a grid line every 10 substrate concentration units, and a grid line every 20 velocity units. . In Excel, gridlines can be controlled under the Chart...
In my lab, we ran a BCA assay for protein concentration and
generated this standard curve, but I have absolutely no idea what
this chart is telling me. Please explain if you can! Thank you.
Curve 3.000 2.500+ 2.000 562 1.500+ 1.000 0.500 0.000+ -500 500 1500 2000 2500 1000 <Plate Layout Settings R2 Curve Curve Name Formula Curve Y=A*X+B Curve Curve Paramet Name Formula er Curve Y=A*X+B A B Fit F Prob 0.00101 0.2020.995 ????? Std. 95% CI 95%...
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