If your cell concentration was 5.5x10^5 cell/mL, how many microliters will dispense 1.1x10^4 cells total? Show your setup and calculation! (1mL = 1000uL)
If your cell concentration was 5.5x10^5 cell/mL, how many microliters will dispense 1.1x10^4 cells total? Show...
For your experiment, you prepared 2ml of cells with a concentration of 2x106/ml (Tube F). You would like to use this cell's solution to prepare a 7ml flask (Tube G) of cells with a concentration of 5x105cells/ml. What volume in microliters of cell solution will you take from tube F into tube G?
1. You have a bacterial cell culture with a concentration of 1x109 cells/ml. You need to dilute the cells to a concentration of 1x103. What is the correct ratio for this dilution? 2. You add 0.1 ml of a yeast culture to a test tube that contains 9.9 ml of buffer solution. What is the dilution factor of this mixture? 3. In a 1000 µl total dilution volume, the volume of cell culture used is 800 µl. What is the...
Your stock cell concentration after couting your cells is 2 x 10^6 cell/ml. What volume of cell suspension contains 200,000 cells?
1a Your cell suspension is 6.3 x 10^8 cells /mL. Explain how to prepare a tube of 1 mL with cells at 2 x 10^5 cells/mL. 1b Now that you have your tube of 1 mL with cells at 2 x 10^5/mL, explain how to add 10^4 cells to each of 10 wells. Do you have enough cells?
You have a yeast cell culture with a concentration of 5x10^4 cells/ml. If you dilute this culture in a 1:100 ratio, what will the concentration be? 5x10^1 5x10^2 5x10^3 5x10^4 None of the above
Your cell suspension is 6.3 x 10^8 cells /mL. Explain how to prepare a tube of 1 mL with cells at 2 x 10^5 cells/mL. Pipetting less than 1 μL is hard to do accurately. Do a dilution if less than 1μL After dilution,Now that you have your tube of 1 mL with cells at 2 x 10^5/mL, explain how to add 10^4 cells to each of 10 wells. Do you have enough cells?
PLEASE HELP SOLVE BOTH QUESTIONS, show all work and circle answers
thanks !
4. A student has taken 1 ml of his cell suspension at 8.5 x 104 cells/ml into 4 mls of media in order to plate them in a new culture vessel. What is the concentration of cells at the start of the new culture? (1 point) 5. A student has a cell suspension of 1.5 X 109 cells/ml. How is she going to prepare 5 separate cultures...
you want obtain a cell count of 3x10^5 cells/ml and want to seed cells at 2x10^4 cells/ml. what is the dilution?
You calculated the live cell concentration to be 1.5 x 10^6 cells/mL. what is the volume of cell suspension needed to seed a T-75 flask with 7.5 x 10^5 cells in 10 mLs of media.
If you have a stock culture of bacteria at a concentration of 5*10 cells/ml, how much stock cuiture and how much media would you add to obtain a final concentration of 2.5*10 cells/ml in a final volume of 20mL? (1 point)