you want obtain a cell count of 3x10^5 cells/ml and want to seed cells at 2x10^4...
you want obtain a cell count of 3x10^5 cells/ml and want to seed cells at 2x10^4 cells/ml. what is the dilution?
Homework Answers
This can be calculated using the formula
V1C1 = V2C2
Where
V1 = Stock volume to be used for dilution = X
C1 = Initial concentration = 3x10^5
V2 = Final volume = 1 ml
C2 = Final concentration = 2x10^4
In the given problem we have to find out the stock volume i.e., V1
So, we shall use the following formula
V1C1 = V2C2
V1 = (V2C2) / C1
V1 = (1 x 2x10^4) / 3x10^5
V1 = 0.66 x 10-1
V1 = 0.066
So, you have to take 0.066 ml of culture which gave 3x10^5 cells/ml and add 0.934 ml of distilled water. This will give cells approximately equal to 2x10^4 cells/mL.
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