5. A _____sequence is the most common binding sequence for a particular transcription factor.
Transcription factors bind to the promoter or enhancer of the genes that they regulate. These are specific DNA sequences known as the transcription factor binding site or the response element.
The TATA box is the most common binding sequence for the transcription factors in eukaryotes.
5. A _____sequence is the most common binding sequence for a particular transcription factor.
Which type of transcription factor would bind to a DNA segment with the following sequence? What is the structure of the DNA binding domain of the protein? What molecular events does binding of the protein promote? 5’ TATATATA 3’ 3’ ATATATAT 5’
The LacI protein is released from the operator sequence upon binding of IPTG, allowing the transcription of all the genes within the lac operon. In this case, LacI is called a transcriptional _______. In comparison, CRP binds to the CRP binding site sequence upon binding of cAMP, enhancing transcription. CRP is called a transcriptional _______. Select one: a. activator ;;;;; activator b. activator ;;;;; repressor c. repressor ;;;;; activator d. repressor ;;;;; repressor e. None of these
The hunchback gene has an enhancer containing 3 binding sites for the transcription factor Bicoid. How could the loss of a single DNA binding site -leaving the 2 others intact- act to reduce gene expression of hunchback by more than 1/3 ? A. Bound Bicoid transcription factor molecules interact antagonistically to cause maximal gene expression B. Bound Bicoid transcription factor molecules interact additively to cause maximal gene expression C. Bicoid does not bind to the hunchback enhancer D. Bound Bicoid...
How will you determine the transcription factor binding sites in the whole genome? Give at least one example from literature.
What is the strand of RNA that will be produced during transcription from this particular sequence of DNA TGAGCCAAACCA? Question 13 2 pts What is the strand of RNA that will be produced during transcription from this partial sequence of DNA: TGAGCCAAACCA? ACUCGGUUUGGU O ACUCGGTUTGGT O ACUCGGUUUCGU O ACTCGGTTTGGT
Transcription regulators control transcription by phosphorylating RNA polymerase. binding to specific sequences of DNA. binding to specific sequences of RNA. degrading mRNAs.
1) You have transfected siRNA against transcription factor A into cells that normally express the transcription factor. These cells also contain a luciferase reporter gene that is controlled an enhancer that has multiple binding sites for transcription factor A. a. What would be the effect on reporter gene activity b. How could you determine whether RNAi acts at the level of mRNA or translation in this case?
Certain laboratories often need to prepare DNA duplexes for transcription factor binding assays. The DNA is purchased as single-stranded polynucleotides and hybridize them to form our duplexes, but this almost always leaves behind some un-renatured, single-stranded DNA, in the solution. How would you go about removing the single-stranded DNA fromour duplexes?
How does ribosome binding affect the final level of protein produced from a DNA sequence? Only one question at a time; only one chance to answer. Question 1 0.5 pts How does ribosome binding affect the final level of protein produced from a DNA sequence? determines the efficiency of transcription factor bindings determines how many proteins can be made from each mRNA determines mRNA stabiity determines teh rate of nuclear transport of mRNAS
. If we increase the concentration of NaCl how will change the binding of our transcription factor to the DNA? How will change the melting point of DNA? Of course, explain.