Which HPLC method provides the best estimation of protein concentration: ion exchange chromatography or size exclusion...
Which HPLC method provides the best estimation of protein concentration: ion exchange chromatography or size exclusion chromatography? Why?
Homework Answers
Solution:
size exclusion chromatography can be preferred over ion exchange chromatography as The advantages of this method include good separation of large molecules from the small molecules with a minimal volume of eluate, and that various solutions can be applied without interfering with the filtration process, all while preserving the biological activity of the particles to be separated.
Thank you
Wish you all the best buddy please upvote it ?
Add Answer to:
Which HPLC method provides the best estimation of protein
concentration: ion exchange chromatography or size exclusion...
A protein is purified from a bacterium using Size Exclusion Chromatography (SEC), with a molecular weight...
A protein is purified from a bacterium using Size Exclusion Chromatography (SEC), with a molecular weight of 200kD. When this protein is run on SDS-PAGE , a sing band is observed at 100kD. Based on these observations, what can be concluded about the structure of the protein? The protein consist of two 200kD subiunits The protein has a quaternary structure The protein is madeup of two 100 kD subunits Proteins is a tetramer consisting of 200 kD domains. The protein...
Which method would work best to isolate protein Z from this mixture of proteins? Protein M.W. ...
Which method would work best to isolate protein Z from this mixture of proteins? Protein M.W. pI X 20,000 6.1 Y 75,000 9.1 Z 110,000 4.6 Answer choices: a. cation exchange at pH=7 b. size-exclusion P100 gel (5,000-100,000 MW fractionation range) c. anion exchange at pH=7 d. size-exclusion P60 gel (3,000-60,000 MW fractionation range)
Suggest the best method of chromatography to separate each of the following pairs: a. protein from...
Suggest the best method of chromatography to separate each of the following pairs: a. protein from a salt b. peptide fragments obtained after protease digestion of a protein from the intact protein c. two isozymes differing by one arginine (positively charged) residue d. antibodies that bind to two different antigens e. two peptides with slightly different amounts of hydrophobic amino acids f. DNA from protein So far I think C is Ion exchange, D is affinity, and E is hydrophobic...
i. Chromatography scale up by a factor of 100 for a linear gradient ion exchange chromatography...
i. Chromatography scale up by a factor of 100 for a linear gradient ion exchange chromatography of a product protein from the laboratory to the plant. The conditions are 2 cm (in diameter) x 20 cm bed height, 20 um particle size and 30 cm/h superficial velocity. The particle size of the same type of ion exchange resin available for the plant operation is 60 um. Two columns are available in the plant: 14 cm in diameter x 50 cm...
To seperate protein X using ion exchange chromatography, I am using a resin with carboxylates attached...
To seperate protein X using ion exchange chromatography, I am using a resin with carboxylates attached (pKa is 6.0). If I wanted to seperate protein X, whose pl is 3.0x what is the pH range of the buffer that can be used for ion exchange chromatography with this resin ? A. any pH between 3.0 and 6.0 B. there is no pH range for this resin and protein X C. any pH above 4.0 D. any pH below 6.0
A purified protein has a molecular mass of 360 kDa when measured by size exclusion chromatography....
A purified protein has a molecular mass of 360 kDa when measured by size exclusion chromatography. When analyzed by gel electrophoresis in the presence of SDS, three bands are observed, with molecular masses of 160, 140, and 60 kDa. When gel electrophoresis is carried out in the presence of SDS and dithiothreitol three bands are once again observed, with molecular masses of 140, 80, and 60 kDa. What is the subunit composition of the protein?
aestion 7 8 poir Find the chromatography method for following description. Solute equilibrates between the mobile...
aestion 7 8 poir Find the chromatography method for following description. Solute equilibrates between the mobile phase and the surface of the solid stationary phase. ion-exchange chromatography A. partition chromatography adsorption chromatography C. o size exclusion chromatography
ANSWER ALL 1) Which of the following is the stationary phase in size exclusion chromatography? A....
ANSWER ALL 1) Which of the following is the stationary phase in size exclusion chromatography? A. resin B. mixture of proteins C. solvent or buffer D. none of these 2) Suppose you wish to purify a protein that has many positively charged residues from a mixture of proteins. Which of the following columns would elute the protein the fastest? A. column packed with neutral resin B. column packed with positively charged resin C. column packed with negatively charged resin D....
Each of the following statements describes a type of column chromatography. Sort the statements to the...
Each of the following statements describes a type of column chromatography. Sort the statements to the type of chromatography they describe. If a statement can describe all of the types, place that statement in the "All" category. (Note that size-exclusion chromatography may also be called gel-filtration or molecular exclusion chromatography.) Categories: Size-exclusion Chromatography, Affinity Chromatography, Ion-exchange Chromatogrpahy, and All Statements: Separate molecules by size. Separate molecules by charge. The stationary phase has a covalently bound group to which a protein...
In order to separate protein X using ion exchange chromatography, I am using a resin with...
In order to separate protein X using ion exchange chromatography, I am using a resin with carboxylates attached (pka is 6.0). If I wanted to separate protein X, whose plis 8.0, what is the pH range of the buffer that can be used for ion exchange chromatography with this resin? any pH between 6.0 and 8.0 any pH above 6.0. any pH below 8.0 there is no pH range for this resin and protein X. Which of the following is...
i. Chromatography scale up by a factor of 100 for a linear gradient ion exchange chromatography of a product protein from the laboratory to the plant. The conditions are 2 cm (in diameter) x 20 cm bed height, 20 um particle size and 30 cm/h superficial velocity. The particle size of the same type of ion exchange resin available for the plant operation is 60 um. Two columns are available in the plant: 14 cm in diameter x 50 cm...
A purified protein has a molecular mass of 360 kDa when measured by size exclusion chromatography. When analyzed by gel electrophoresis in the presence of SDS, three bands are observed, with molecular masses of 160, 140, and 60 kDa. When gel electrophoresis is carried out in the presence of SDS and dithiothreitol three bands are once again observed, with molecular masses of 140, 80, and 60 kDa. What is the subunit composition of the protein?
aestion 7 8 poir Find the chromatography method for following description. Solute equilibrates between the mobile phase and the surface of the solid stationary phase. ion-exchange chromatography A. partition chromatography adsorption chromatography C. o size exclusion chromatography
In order to separate protein X using ion exchange chromatography, I am using a resin with carboxylates attached (pka is 6.0). If I wanted to separate protein X, whose plis 8.0, what is the pH range of the buffer that can be used for ion exchange chromatography with this resin? any pH between 6.0 and 8.0 any pH above 6.0. any pH below 8.0 there is no pH range for this resin and protein X. Which of the following is...
Most questions answered within 3 hours.
-
The blues made its way into many kinds of music. Eric Clapton,
The Beatles, and Elvis...
asked 50 minutes ago -
If you’re standing at the bottom of a hill and asked to evaluate
it while being...
asked 1 hour ago -
1. Which region has taken the lead in the world of
e-waste handling?
a) European Union...
asked 1 hour ago -
A 8.15- g bullet from a 9-mm pistol has a velocity of 366.0 m/s.
It strikes...
asked 3 hours ago -
The outstanding bonds of Alpha Extracts have a yield to maturity
of 7.4 percent and a...
asked 3 hours ago -
The Problem: The Case of the Harmonizing Vacations
Your CEO is exploring partnering with a European...
asked 4 hours ago -
A chemical equation is balanced by adding coefficients in front
of some formulas so that the...
asked 4 hours ago -
From the literature (reference your sources): What are the
lattice parameters of calcite and aragonite? Why...
asked 5 hours ago -
Your system is rejecting the question am asking which is
preceded by a case study. It...
asked 5 hours ago -
3. On January 2, 2000, Larry creates a trust with himself as
trustee. Larry as trustee...
asked 5 hours ago -
A member of the volleyball team spikes the ball. During this
process, she changes the velocity...
asked 5 hours ago -
Are adult gamers less likely to use a gaming console (Xbox,
PlayStation, Wii, etc...) than teen...
asked 6 hours ago