Wild-type bacteria can grow on minimal medium. Four mutants that cannot grow on minimal medium but...
Wild-type bacteria can grow on minimal medium. Four mutants that
cannot grow on minimal medium but can grow on minimal medium
supplemented with the nutrient “C” are isolated. It is suspected
that metabolites A, B, and D are in the biochemical pathway for
synthesis of C, so each mutant is tested for the ability to grow on
minimal medium supplemented with these metabolites:
Mutant 1: can grow on minimal medium supplemented with A, but not B
or D
Mutant 2: is unable to grow on minimal medium supplemented with A,
B, or D
Mutant 3: is able to grow on minimal medium supplemented with B or
A, but not D
Mutant 4: can grow on minimal medium supplemented with A, B, or
D.
Which of the following is NOT consistent with this
information?
Mutant 2 will cause a build up of metabolite D
Mutant 4 blocks a step before all 4 metabolites.
Mutant 2 blocks a step right before metabolite C.
Mutant 3 blocks the conversion of metabolite D → metabolite B
Mutant 1 will cause a build up of metabolite B
Homework Answers
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Wild-type bacteria can grow on minimal medium. Four mutants that
cannot grow on minimal medium but...
Wild-type bacteria can grow on minimal medium. Four mutants that cannot grow on minimal medium but...
Wild-type bacteria can grow on minimal medium. Four mutants that cannot grow on minimal medium but can grow on minimal medium supplemented with the nutrient "H" are isolated. It is suspected that metabolites T, P, and A are in the biochemical pathway for synthesis of H, so each mutant is tested for the ability to grow on minimal medium supplemented with these metabolites: A. Mutant 1: can grow on minimal medium supplemented with T, but not P or A B....
Four nutritional mutants in Neurospora will grow if arginine is added to minimal medium. Some of...
Four nutritional mutants in Neurospora will grow if arginine is added to minimal medium. Some of these mutants also will grow if other substrates chemically related to arginine are added to minimal medium. The growth patterns are show in the table (+ = growth and 0 = no growth). A. What is the sequence in the metabolic pathway of arginine (order of metabolites)? B. Indicate where each mutant is blocked in the pathway? Supplements (metabolites) added to minimal medium...
2. Four auxotrophic mutants in Neurospora crassa.(common bread mold) will grow if arginine is added to...
2. Four auxotrophic mutants in Neurospora crassa.(common bread mold) will grow if arginine is added to minimal medium. Some of these mutants will also grow if other chemicals in the arginine anabolic pathway are added to minimal medium. The growth pattems are show in the table (+ = growth and 0 - no growth) Metabolites added to minimal medium Glutamic Semialdehyde Arginine Mutants Precursor Citrulling . Omithing A. What is the order of metabolites in the pathway? B. Indicate with...
True/False 6. a)A tryptophan auxotrophic strain (trp¯) of bacteria will be able to grow on a...
True/False 6. a)A tryptophan auxotrophic strain (trp¯) of bacteria will be able to grow on a minimal medium agar that has been supplemented with the amino acid histidine. b). Hfr strains result from the insertion and recombination of the F factor plasmid into the bacterial chromosome. c). In generalized transduction, any gene or set of genes on the chromosome may be transferred from the donor bacterium to the recipient bacterium. d). The order of genes on a bacterial chromosome can...
Strain Phenotype on YPD Phenotype on Minimal Wild type Aade2 Made4 Aade2 Aade4 2. (2 pts)...
Strain Phenotype on YPD Phenotype on Minimal Wild type Aade2 Made4 Aade2 Aade4 2. (2 pts) The YPD plates contain a limited amount of adenine, which causes induction of the pathway to synthesize more. Mutation of the genes required for the pathway can lead to blockage at an intermediate stage of the reaction. Accumulation of a certain intermediate leads to a red coloration of the yeast in your Aade2 mutant. The order of the genes involved in the pathway can...
A Lab has isolated a gene, CFE1, that encodes an enzyme (Cfe1) that completes one step...
A Lab has isolated a gene, CFE1, that encodes an enzyme (Cfe1) that completes one step of the conversion of caffeine into adenine. to check if CFE1 might be the gene that is mutated in one of the mutants discovered in the original screen. cross each one of the mutants with a strain that has the CFE1 gene fully deleted (cfe14). allow the resulting diploid strain to go through meiosis and produce haploid gametes (“spores") to individually check for the...
-A scientist is studying a mutant strain of bacteria that cannot synthesize isoleucine. After placing them...
-A scientist is studying a mutant strain of bacteria that cannot
synthesize isoleucine. After placing them in different mediums with
different intermediates, she places some bacterial cells in a
medium with Intermediate C and they survive and produce isoleucine.
Please state which enzymes in this bacteria’s metabolic pathway are
functional and which ones are not.
-In a wild type strain of bacteria, she notices that when
isoleucine accumulates its production starts slowing down, what
type of regulation is this? Where...
III. You isolate a new species of bacteria that only grows in the Antarctic. A wild...
III. You isolate a new species of bacteria that only grows in the Antarctic. A wild type strain of this bacteria can synthesize its own alanine (alat) and its own tryptophan trp' ), both of which are essential amino acids. A mutant strain of the same bacterium cannot synthesize either of those two amino acids (ala trp'). You are interested in the degree of linkage between the ala and the trp genes in this new bacterial species. Fortunately, this species...
5. Made up amino acids Bleucine and Isobleucine are synthesized in a series of steps from...
5. Made up amino acids Bleucine and Isobleucine are synthesized in a series of steps from made up sugar Blorptose. These are essential for the organism to grow but can be supplied in the medium otherwise to complement the growth defect of mutants when amino acids cannot be synthesized. There are a number of chemical intermediates that are each synthesized by enzymes. Some enzymes in the pathway give both Bleu' and Isobleu phenotypes, while others are only Bleu' or Isableu....
3. (7) Suppose that you have selected E cofthat can grow only when the medium is...
3. (7) Suppose that you have selected E cofthat can grow only when the medium is supplemented with cysteine. You isolate one mutant that you believe is defective in a new gene require for cysteine biosynthesis- sX You have two strains of bacteria: Hitr cysX- galehislac+ purtrpefort cysX+gal-his-tac-pur-trp-tort A) What supplements would you need to add to minimal media to recover all cysX lach, fon recombinants? Would you add glucose? B) What recombinant genotypes would you recover on plates containing...
2. Four auxotrophic mutants in Neurospora crassa.(common bread mold) will grow if arginine is added to minimal medium. Some of these mutants will also grow if other chemicals in the arginine anabolic pathway are added to minimal medium. The growth pattems are show in the table (+ = growth and 0 - no growth) Metabolites added to minimal medium Glutamic Semialdehyde Arginine Mutants Precursor Citrulling . Omithing A. What is the order of metabolites in the pathway? B. Indicate with...
Strain Phenotype on YPD Phenotype on Minimal Wild type Aade2 Made4 Aade2 Aade4 2. (2 pts) The YPD plates contain a limited amount of adenine, which causes induction of the pathway to synthesize more. Mutation of the genes required for the pathway can lead to blockage at an intermediate stage of the reaction. Accumulation of a certain intermediate leads to a red coloration of the yeast in your Aade2 mutant. The order of the genes involved in the pathway can...
A Lab has isolated a gene, CFE1, that encodes an enzyme (Cfe1) that completes one step of the conversion of caffeine into adenine. to check if CFE1 might be the gene that is mutated in one of the mutants discovered in the original screen. cross each one of the mutants with a strain that has the CFE1 gene fully deleted (cfe14). allow the resulting diploid strain to go through meiosis and produce haploid gametes (“spores") to individually check for the...
-A scientist is studying a mutant strain of bacteria that cannot
synthesize isoleucine. After placing them in different mediums with
different intermediates, she places some bacterial cells in a
medium with Intermediate C and they survive and produce isoleucine.
Please state which enzymes in this bacteria’s metabolic pathway are
functional and which ones are not.
-In a wild type strain of bacteria, she notices that when
isoleucine accumulates its production starts slowing down, what
type of regulation is this? Where...
III. You isolate a new species of bacteria that only grows in the Antarctic. A wild type strain of this bacteria can synthesize its own alanine (alat) and its own tryptophan trp' ), both of which are essential amino acids. A mutant strain of the same bacterium cannot synthesize either of those two amino acids (ala trp'). You are interested in the degree of linkage between the ala and the trp genes in this new bacterial species. Fortunately, this species...
5. Made up amino acids Bleucine and Isobleucine are synthesized in a series of steps from made up sugar Blorptose. These are essential for the organism to grow but can be supplied in the medium otherwise to complement the growth defect of mutants when amino acids cannot be synthesized. There are a number of chemical intermediates that are each synthesized by enzymes. Some enzymes in the pathway give both Bleu' and Isobleu phenotypes, while others are only Bleu' or Isableu....
3. (7) Suppose that you have selected E cofthat can grow only when the medium is supplemented with cysteine. You isolate one mutant that you believe is defective in a new gene require for cysteine biosynthesis- sX You have two strains of bacteria: Hitr cysX- galehislac+ purtrpefort cysX+gal-his-tac-pur-trp-tort A) What supplements would you need to add to minimal media to recover all cysX lach, fon recombinants? Would you add glucose? B) What recombinant genotypes would you recover on plates containing...
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