What would happen if the enzyme was non-functional for Polymerase, I Polymerase III, Ligase, Helicase and...
What would happen if the enzyme was non-functional for Polymerase, I Polymerase III, Ligase, Helicase and Okazaki Explain please
Homework Answers
during DNA replication all the step are important ,if the enzyme become non functional for them them then in the absence of these enzyme there shall be no polymerization of nucleotides take place.as a results many error occur which form mismatch and causes mutation.
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What would happen if the enzyme was non-functional for
Polymerase, I Polymerase III, Ligase, Helicase and...
Describe the roles of DNA polymerase I, DNA polymerase III, gyrase, helicase, primase, and ligase in...
Describe the roles of DNA polymerase I, DNA polymerase III, gyrase, helicase, primase, and ligase in the replication of E. coli DNA. What features of the E. coli replisome and of DNA polymerase III’s structure are associated with replication processivity? How do mammalian cells prime discontinuous strand replication and how do they remove RNA primers?
Match the enzyme activity in DNA synthesis with its function. DNA pol III (_) fragments Helicase...
Match the enzyme activity in DNA synthesis with its function. DNA pol III (_) fragments Helicase (_) Primase (_) using template strand DNA pol I (_) double helix DNA ligase (_) adds nucleotides Topoisomerase (_) primer A. anneals DNA B. relieves overwinding C. adds nucleotides D. unwinds parental E. removes RNA primer, F. adds a short RNA 10 Proofreading and repair of the DNA double helix does NOT involves a. detecting a mis-match in base pairs. b. removing the mis-match...
Describe the process of DNA replication in a well-organized manner. Start with the helicase and go...
Describe the process of DNA replication in a well-organized manner. Start with the helicase and go through the entire process, step by step. Use these terms correctly: Helicase, DNA primase, single stranded binding proteins, DNA polymerase I, DNA Polymerase III, leading strand, lagging strand, okazaki fragments, ligase, topoisomerase, sliding clamp, clamp loader. Please use correct descriptions of the enzyme function. For example, do not say, “seals nicks.” Tell me what “seals nicks” means. If you speak about something you have...
DNA Replication - Describe the process of DNA replication in a well-organized manner. Start with the...
DNA Replication - Describe the process of DNA replication in a well-organized manner. Start with the helicase and go through the entire process, step by step. Use these terms correctly: Helicase, DNA primase, single stranded binding proteins, DNA polymerase I, DNA Polymerase III, leading strand, lagging strand, okazaki fragments, ligase, topoisomerase, sliding clamp, clamp loader. Please use correct descriptions of the enzyme function. For example, do not say, “seals nicks.” Tell me what “seals nicks” means.
Matching the normal function to the enzyme. This enzyme connects Okazaki fragments by forming bonds in...
Matching the normal function to the enzyme. This enzyme connects Okazaki fragments by forming bonds in the A. DNA backbone. Topoisomerase This enzyme opens the DNA helix, separating the strands to expose B. the bases. Primase This enzyme relaxes supercoils in the DNA that form ahead of the replication fork Helicase DNA Polymerase III This enzyme removes primers by cutting out one RNA nucleotide at a D. time and replacing it with DNA. DNA Polymerase This enzyme uses its own...
D Question 1 (Matching) Match the enzyme/structure with its role in DNA replication. DNA polymerase synthesizes...
D Question 1 (Matching) Match the enzyme/structure with its role in DNA replication. DNA polymerase synthesizes the new strand of RNA primase Choose synthesizes the new strand of DNA synthesizes a short fragment of complementary RNA attach to template DNA strands to prevent hydrogen bonding Uncoils the supercoil of prokaryotic chromosomes connects Okazaki fragments of new lagging strand of DNA unwinds and unzips the double stranded DNA Single stranded binding proteins (SSB's) DNA Ligase [Choose] Helicase [Choose) DNA Gyrase (Choose]...
1. Which of the following statements applies to the 5' - 3' exonuclease activity of DNA...
1. Which of the following statements applies to the 5' - 3' exonuclease activity of DNA polymerase I? You may select multiple answers. a. It allows the enzyme to remove the primer from the primer's 5' end. b. It allows the enzyme to proofread itself. c. It cuts phosphodiester bonds. d. It is used to synthesize a new DNA molecule. e. It is used to seal together Okazaki fragments. 2. Which enzyme are able to proofread themselves? Select all that...
Question 5 1 pts The enzyme helicase is an important part of the replication machinery, but...
Question 5 1 pts The enzyme helicase is an important part of the replication machinery, but its use now requires specifically the activity of the following enzyme: Topoisomerase ODNA polymerase Sliding clamp Primase Ligase Question 7 1 pts Much of replication can be studied using extracts from wild type (normal) yeast cells that supply all of the essential components. Compared to wild type extracts, if you use an extract that is missing the sliding clamp AND ligase, what do you...
the grey are the options Please match each enzyme with the proper function Primase Helicase ✓...
the grey are the options
Please match each enzyme with the proper function Primase Helicase ✓ Choose catalyzes the formation of phosphodiester bonds between DNA fragments. unwinds supercolled DNA by catalyzing the controlled Cleavage and rejoining of DNA. coats DNA, preventing duplex formation. removes RNA primers and replaces them with DNA nucleotides. breaks hydrogen bonds, unwinding DNA. synthesizes short sequences of RNA required for DNA replication starts DNA synthesis at the end of a primer. Single-stranded binding protein DNA polymerase...
1. In class we discussed temperature sensitive mutants that are functional at lower temperatures but non-functional...
1. In class we discussed temperature sensitive mutants that are functional at lower temperatures but non-functional at higher temperatures (e.g. above 42 "C). Although less common, there are also examples of cold sensitive mutants that are functional at higher temperatures but non functional at lower temperatures (eg. below 28 °C). Instead of performing a normal pulse-chase experiment, we can combine the pulse-chase with a temperature shift. The experiment is performed by first growing cells at a permissive temperature (30-35 °C)...
Match the enzyme activity in DNA synthesis with its function. DNA pol III (_) fragments Helicase (_) Primase (_) using template strand DNA pol I (_) double helix DNA ligase (_) adds nucleotides Topoisomerase (_) primer A. anneals DNA B. relieves overwinding C. adds nucleotides D. unwinds parental E. removes RNA primer, F. adds a short RNA 10 Proofreading and repair of the DNA double helix does NOT involves a. detecting a mis-match in base pairs. b. removing the mis-match...
Matching the normal function to the enzyme. This enzyme connects Okazaki fragments by forming bonds in the A. DNA backbone. Topoisomerase This enzyme opens the DNA helix, separating the strands to expose B. the bases. Primase This enzyme relaxes supercoils in the DNA that form ahead of the replication fork Helicase DNA Polymerase III This enzyme removes primers by cutting out one RNA nucleotide at a D. time and replacing it with DNA. DNA Polymerase This enzyme uses its own...
D Question 1 (Matching) Match the enzyme/structure with its role in DNA replication. DNA polymerase synthesizes the new strand of RNA primase Choose synthesizes the new strand of DNA synthesizes a short fragment of complementary RNA attach to template DNA strands to prevent hydrogen bonding Uncoils the supercoil of prokaryotic chromosomes connects Okazaki fragments of new lagging strand of DNA unwinds and unzips the double stranded DNA Single stranded binding proteins (SSB's) DNA Ligase [Choose] Helicase [Choose) DNA Gyrase (Choose]...
Question 5 1 pts The enzyme helicase is an important part of the replication machinery, but its use now requires specifically the activity of the following enzyme: Topoisomerase ODNA polymerase Sliding clamp Primase Ligase Question 7 1 pts Much of replication can be studied using extracts from wild type (normal) yeast cells that supply all of the essential components. Compared to wild type extracts, if you use an extract that is missing the sliding clamp AND ligase, what do you...
the grey are the options
Please match each enzyme with the proper function Primase Helicase ✓ Choose catalyzes the formation of phosphodiester bonds between DNA fragments. unwinds supercolled DNA by catalyzing the controlled Cleavage and rejoining of DNA. coats DNA, preventing duplex formation. removes RNA primers and replaces them with DNA nucleotides. breaks hydrogen bonds, unwinding DNA. synthesizes short sequences of RNA required for DNA replication starts DNA synthesis at the end of a primer. Single-stranded binding protein DNA polymerase...
1. In class we discussed temperature sensitive mutants that are functional at lower temperatures but non-functional at higher temperatures (e.g. above 42 "C). Although less common, there are also examples of cold sensitive mutants that are functional at higher temperatures but non functional at lower temperatures (eg. below 28 °C). Instead of performing a normal pulse-chase experiment, we can combine the pulse-chase with a temperature shift. The experiment is performed by first growing cells at a permissive temperature (30-35 °C)...
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