As with question 1, below is some sequence around the region of the PER3 gene that...
As with question 1, below is some sequence around the region of the PER3 gene that you amplified by PCR in Practical class 4. This contains five of the 54bp repeats. TCCTATCACA GGCTGGAAGC AATAAGACAG CTCAAAATTT TATGACACTA CCAGAATGGC TGACAATTTT AAACTTATGA ACTGTTTATT TCTGGAATTT TCCATTTACT ATTTTTGGAC CTTGGTTGAC CACAGGTAAC TGAAACTACA GAAAGCAAAA CCATGAATAA AGGAGGACTA CTGTATTTTG TGATAAGAAG ATTAAAGTGT CTTTTCATGT GCCCTTACTT TCTAGCAGTG TGTTACAGGC AACAATGGCA GTGAGAGCAG TCCTGCTACT ACCGGTGCAC TGTCCACGGG GTCACCTCCC AGGGAGAATC CATCCCATCC TACTGCCAGC GCTCTGTCCA CAGGATCGCC TCCCATGAAG AATCCATCCC ATCCTACTGC CAGCGCTCTG TCCACAGGAT CGCCTCCCAT GAAGAATCCA TCCCATCCTA CTGCCAGCAC ACTGTCCATG GGATTGCCTC CCAGCAGGAC TCCATCCCAT CCTACTGCCA CTGTTCTGTC CACGGGGTCA CCTCCCAGCG AATCCCCATC CAGAACTGGT TCAGCAGCAT CAGGTAGTGG ATCAGGACAA CTAATGTTTC AAACTCCAAT GCCAGACATT CACTATGTGC TGAGCTCTCA CTGTGTGCCC CAGGCACTGA TGTGGAAGTA CAAGGTTTTT TTTTCTTTTT CCCTTTTTCC TTTTTGTCAG GTATATTGGG GTATATTTAT ACACAATAAA ATTCACCAGT TTGAGGGGTA CAAACAAGAA TTCTGACAGA TGTGTACCTT AGTGTAAGCG CTACCACAAT CACCATA The 2 primers used in this PCR were as follows: PER3F 5' CAAAATTTTATGACACTACCAGAATGGCTGAC 3' PER3R 5' AACCTTGTACTTCCACATCAGTGCCTGG 3' What would the size of the PCR product be if the individual who supplied the DNA template was homozygous for the four repeat allele?
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As with question 1, below is some sequence around the region of
the PER3 gene that...
Animal Science Cell and Molecular Biology Quesiton -- The bolded sequence in the sequence in question...
Animal Science Cell and Molecular Biology Quesiton --
The bolded sequence in the sequence in question 5 is a variable
number tandem repeat sequence (VNTR or STR). The following table
shows the PCR product lengths amplified using the primers you
designed in question 5 and DNA samples from four sets of skeletal
remains found buried in an archaeological excavation. List the VNTR
lengths for the two alleles for each skeleton in the table
below.
5. Design 15 nucleotide long PCR...
Below is a sequence of double-stranded DNA that will be used as a template in the...
Below is a sequence of double-stranded DNA that will be used as a template in the polymerase chain reaction (PCR). The goal is to use this as a template to make a a PCR product that includes the shaded area. The sequence of one of the PCR primers is given below. Template DNA: 5' - CTTAGCGCTGTTGGGGGCCAACTATCACACACACCACACACAGGTATAAATGGCATTTGATACAGATTG - 3' 3' - GAATCTGTATCAAATGCCATTTATACCTGTGTGTGGTGTGTGTGATAGTTGGCCCCCAACAGCGCTAAG - 5' If the sequence of one of the primers is 5' TTGTGGGGCC 3' which of the following primers...
2. PCR amplification of the TAS2R38 gene a. The number of copies of the 303 bp sequence grows exp...
2. PCR amplification of the TAS2R38 gene a. The number of copies of the 303 bp sequence grows exponentially (1-2-4-8-etc) after each cycle. The number of cycles we used is on page 97. What is the number of copies of the 303 bp fragment that will theoretically be present at the end of our reaction? b. Denaturation of the 303 bp segment of the TAS2R38 gene is a critical first step in the PCR perties of a DNA segment that...
The following is the DNA sequence of the wild type allele of genr Z that you...
The
following is the DNA sequence of the wild type allele of genr Z
that you want to amplify using the polymerase chain reaction (PCR).
Circle the set(s) of primers from the options below, which you
would use for PCR reaction. Circle all that apply.
10. The following is the DNA sequence of the wild type allele of Gene Z that you want to amplify using the polymerase chain reaction (PCR). 5' CTCGAGGTGAATATGAAAG---- 3'GAGCTCCACTTATACTTTC---- Gene Z ---CATTTGGCGCGTAATCGATA3 ---GTAAACCGCGCATTAGCTATS! Circle the...
Huntington's disease (HD) is a late-onset fatal genetic disorder that causes the progressive breakdown of nerve...
Huntington's disease (HD) is a late-onset fatal genetic disorder that causes the progressive breakdown of nerve cells in the brain. The disease is caused by an autosomal dominant mutation whereby an expansion of a CAG trinucleotide repeat is observed in the gene Huntingtin (HTT. Where normal individuals have up to 35 copies of the triplet, individuals with repeat regions containing more than 35 repeats are susceptible to HD, and all disease alleles with 42 or more repeats are completely penetrant....
QUESTION 1: You are inserting a gene into an MCS found within the LacZ gene. Using...
QUESTION 1: You are inserting a gene into an MCS found within the LacZ gene. Using blue/white colony selection, why could you assume that white colonies have modified plasmids? a. A blue colony means the LacZ reading-frame was disrupted b. A blue colony means your gene has mutations c. A white colony means the LacZ reading-frame is intact d. A white colony means the LacZ reading-frame was disrupted QUESTION 2: You are performing a PCR using primers with a sequence perfectly...
region is a DNA sequence that regulates transcription. Within a gene, a region is a DNA...
region is a DNA sequence that regulates transcription. Within a gene, a region is a DNA sequence that encodes RNA and a o coding, barcoding o control, coding o noncoding, control o coding, control During transcription, the DNA template is read in the ___direction. o 3 to 5 oC to N terminal ON to C terminal 0 5' to 3' For genes encoding protein, which of the following is the eukaryotic consensus sequence of the promoter? ATAT o GCGC CGCG...
3. The insertion of a 4,700-bp Doc retroposon is in the MIDDLE of the promoter region...
3. The insertion of a 4,700-bp Doc retroposon is in the MIDDLE of the promoter region of the white gene as indicated between P1 and P3 (467bp) in Figure below. It is the same distance between P1 and point A (the beginning of retroposon) and between point C (the end of retroposon) as and P3. The retroposon specific primer P2 is 100 bp downstream of A. Use Primer sets P1, P2, P3 to amplify the white one gene promoter and...
Please help with all questions. I provided all the information that I have. The sequence below...
Please help with all questions. I provided all the information that I have. The sequence below represents the genomic DNA sequence of the first 440 bp of your gene of interest (exon 1 in blue). You want to amplify this full 440 bp region by PCR, for cloning into a plasmid vector. tgaagtccaactcctaagccagtgccagaagagccaaggacaggtacggctgtcatcacttagacctcaccctgtggagccacaccctagggttggccaatctactcccaggagcagggagggcaggagccagggctgggcataaaagtcagggcagagccatctattgcttacatttgcttctgacacaactgtgttcactagcaacctcaaacagacaccatggtgcatctgactcctgaggagaagtctgccgttactgccctgtggggcaaggtgaacgtggatgaagttggtggtgaggccctgggcaggttgctatcaaggttacaagacaggtttaaggagaccaatagaaactgggcatgtggagacagagaagactcttgggtttctgataggcactgactctctctgcctattggtctattttcccaccc 1.1 Design a 20 nucleotide forward & reverse primer set that will allow you to amplify the sequence above. (note - primers should be at the beginning...
based on the PCR results, estimate how mang CTG repeats are present in rhe DMPK gene...
based on the PCR results, estimate how mang CTG repeats are
present in rhe DMPK gene of parient 1:
part b: why are there 2 different sized bands detected in patient
2?
a onown below is DNA sequence from the 3'UTR of the DMPK gene, where the bold (CTG) is repeated 20 times in healthy patients and 50-1,500 times in patients with Myotonic Dystrophy. Using single letter abbreviations, write the first 3 nucleotides of both primers you would need to...
Animal Science Cell and Molecular Biology Quesiton --
The bolded sequence in the sequence in question 5 is a variable
number tandem repeat sequence (VNTR or STR). The following table
shows the PCR product lengths amplified using the primers you
designed in question 5 and DNA samples from four sets of skeletal
remains found buried in an archaeological excavation. List the VNTR
lengths for the two alleles for each skeleton in the table
below.
5. Design 15 nucleotide long PCR...
2. PCR amplification of the TAS2R38 gene a. The number of copies of the 303 bp sequence grows exponentially (1-2-4-8-etc) after each cycle. The number of cycles we used is on page 97. What is the number of copies of the 303 bp fragment that will theoretically be present at the end of our reaction? b. Denaturation of the 303 bp segment of the TAS2R38 gene is a critical first step in the PCR perties of a DNA segment that...
The
following is the DNA sequence of the wild type allele of genr Z
that you want to amplify using the polymerase chain reaction (PCR).
Circle the set(s) of primers from the options below, which you
would use for PCR reaction. Circle all that apply.
10. The following is the DNA sequence of the wild type allele of Gene Z that you want to amplify using the polymerase chain reaction (PCR). 5' CTCGAGGTGAATATGAAAG---- 3'GAGCTCCACTTATACTTTC---- Gene Z ---CATTTGGCGCGTAATCGATA3 ---GTAAACCGCGCATTAGCTATS! Circle the...
Huntington's disease (HD) is a late-onset fatal genetic disorder that causes the progressive breakdown of nerve cells in the brain. The disease is caused by an autosomal dominant mutation whereby an expansion of a CAG trinucleotide repeat is observed in the gene Huntingtin (HTT. Where normal individuals have up to 35 copies of the triplet, individuals with repeat regions containing more than 35 repeats are susceptible to HD, and all disease alleles with 42 or more repeats are completely penetrant....
region is a DNA sequence that regulates transcription. Within a gene, a region is a DNA sequence that encodes RNA and a o coding, barcoding o control, coding o noncoding, control o coding, control During transcription, the DNA template is read in the ___direction. o 3 to 5 oC to N terminal ON to C terminal 0 5' to 3' For genes encoding protein, which of the following is the eukaryotic consensus sequence of the promoter? ATAT o GCGC CGCG...
3. The insertion of a 4,700-bp Doc retroposon is in the MIDDLE of the promoter region of the white gene as indicated between P1 and P3 (467bp) in Figure below. It is the same distance between P1 and point A (the beginning of retroposon) and between point C (the end of retroposon) as and P3. The retroposon specific primer P2 is 100 bp downstream of A. Use Primer sets P1, P2, P3 to amplify the white one gene promoter and...
based on the PCR results, estimate how mang CTG repeats are
present in rhe DMPK gene of parient 1:
part b: why are there 2 different sized bands detected in patient
2?
a onown below is DNA sequence from the 3'UTR of the DMPK gene, where the bold (CTG) is repeated 20 times in healthy patients and 50-1,500 times in patients with Myotonic Dystrophy. Using single letter abbreviations, write the first 3 nucleotides of both primers you would need to...
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