a protein sample was diluted 1/5 and then 5ml of the diluted sample was mixed with 20 ml of reactants to give 25 ml total volume. from this 25 ml solution, 5ml were removed. this 5ml contain 3 mg of protein. what was the concentration of protein in the original sample
5 ml of the sample has 3 mg protein.
Therefore the 25 ml solution on the whole contains 5*3mg of protein =15 mg of protein.
5 ml which was taken from 1/5th dilution contains 15 mg protein. Thus, the original sample should contain 5 times of 15 mg protein
=75 mg of protein.
Thus the original sample contains 75 mg of protein
a protein sample was diluted 1/5 and then 5ml of the diluted sample was mixed with...
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In lab, you diluted your unknown protein solution by combining
14 ?L of unknown protein solution with enough
PBS buffer to obtain a total volume of 100 ?L, then added 1000 ?L Bradford reagent. After waiting ten
minutes, the absorbance at 595 nm was 0.206. Using the standard
curve from the previous question, calculate the total concentration
of protein (in ?gmL) in the original sample.
Slope = 0.02371 Intercept = 0.01132 r2
= 0.973
You need to load 2mg total protein in 10ml (i.e. final protein concentration needed is 0.2mg/ml for all the samples). You need to prepare 20 ml of this solution that has protein and 1X sample buffer (water is used for dilution). Complete the table below. Sample Protein Concentration mg/mL Protein Sample (microliter) Water (microliter) 4X Sample Buffer (microliter) Total Sample Volume (microliter) Total Lysate (-) 1 mg/mL 20 E1 0.07 mg/mL 20 E2 0.15 mg/mL 20 E3 0.2 mg/mL 20