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Explain how step 2 of the Citric acid cycle acts as a setup reaction for the...

  1. Explain how step 2 of the Citric acid cycle acts as a setup reaction for the oxidative decarboxylation reaction in step 3.
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Citric acid cycle also known as tricorboxylic acid cycle (TCA) or Krebs cycle is the important pathway of cellular respiration. It uses acetyl CO A released out of oxidation of pyruvate as a starting compound to produce ATP or energy by series of redox reactions.

In the second step citrate is coming converted to isocitrate by removal and addition of H20. In step 3 Isocitrate is the substrate for isocitrate dehydrogenase which oxidises isocitrate and release C02 and alpha keto glutarate which is a five carbon compound. Since isocitrate dehydrogenase make use of isocitrate only, Aconitase in step 2 interconvert citrate to isocitrate creating the setup for step 3.

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