Question

Use the DNA sequence below and the genetic code in your text to answer the following...

Use the DNA sequence below and the genetic code in your text to answer the following questions.

Template DNA 3ʹ T A A G C G A T A C G A G A 5ʹ

Non-template DNA 5ʹ A T T C G C T A T G C T C T 3ʹ

What is the RNA sequence that would be transcribed from the sequence above? Include the 3ʹ and 5ʹ ends. (0.5)

b) What is the amino acid sequence that would be translated from the RNA sequence? (0.5)

c) Add one C nucleotide at the position indicated by the arrow. Rewrite the new DNA molecule, with the additional base pair, and include the 3ʹ and 5ʹ ends. (0.5) d) What is the RNA sequence (include the 3ʹ and 5ʹ ends) and the amino acid sequence that would be derived from the DNA sequence in question (c)?

e) What type(s) of mutation(s) is/are observed? (0.5)

f) Describe in some detail 3 different outcomes of this type of mutation and the potential impact on the organism from the smallest change to the largest change. Be quite specific about what happens to the protein in each case. (3)

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