Differentiate between serial dilution, a simple dilution and a compound dilution
Differentiate between serial dilution, a simple dilution and a compound dilution
You have performed a serial dilution of a sample with an unknown concentration of microorganisms. You counted 55 CFU on a countable plate inoculated with 0.1 mL of a 10-3 dilution. What was the population density of the original sample per milliliter? Many of the quantitative methods discussed are popular because they enable the microbiology researcher to selectively count live cells only. Why do you think this might be an important or desirable feature in a medical or environmental research...
Serial Dilution Questions
1, Serial dilution Questions: a) (5 pts) Suppose you begin with stock solution of dye that has a concentration of 350.0 mg/L and do the following set of serial dilutions on it: 5.00 mL of stock diluted to a total volume (TV) of 500.00 mL (Dilution 1); 2,00 mL of Dilution 1 is then diluted to a TV of 250.00 mL (Dilution 2); finally, 1.00 mL of Dilution 2 is then diluted to a total volume of...
diagram a 3 fold serial dilution series out to a 1:243 dilution. include the dilution at each step.
Explain this example of the following water quality serial dilution. Note the dilution factors and how you would calculate #;s from the incubated plates. A serial dilution yielded TBTC in the 1:1 plate and the 1:10 plate, 10 cfu’s in the 1:100 plate and 1 cfu’s in the 1:1000 plate. What is the concentration (CFU)/ml of the raw sample? Is water acceptable for portable or Recreational use? Explain. When do you use your filtration rather than serial dilution?
Give an example of a serial dilution, provide a diagram and calculation to demonstrate your understanding of a serial dilution
7. What is the final dilution factor of a 4 fold serial dilution with 5 total tubes including the "neat" tube? (neat means undiluted or the original specimen) 1:4 2 Neat ? Dilution factor
7. What is the final dilution factor of a 4 fold serial dilution with 5 total tubes including the "neat" tube? (neat means undiluted or the original specimen) 1:4 2 Neat ? Dilution factor
The following details the results of a serial dilution of E. coli: Effective Dilution Factor on Plate CFUs 105 1095 106 178 107 5 108 None The "effective dilution factor on plate" already takes into account the fact that you plated 0.1 ml of the dilution. How many CFUs/ml were present in the original sample? a. 1.78 x 10^8 b. 1.14 x 10^8 c. 1.125 x 10^8 d. 1.095 x 10^8
Using a serial dilution with three steps, how can you make a 1:5000 dilution? Show two different approaches you can take (each using three steps/dilutions). Could someone please guide me through the steps of how to solve this dilution problem? I'm at a loss as to where to start
3. A serial dilution of a swab sample was prepared by adding 10 uL to a volume of 990 ML water in tube 1, and 10 uL to a volume of 990 uL water in tube 2. a. What is the overall dilution and dilution factor of each tube? Overall Dilution Dilution Factor Tube 1 Tube 2 b. If 100 uL of tube 2 is spread plated and 126 colonies grow, what was the #cells/mL in the original sample?
Practice problem F5: Design a serial dilution that has at least three steps and ends with a DF of 10. The smallest volume that can be transferred between any two tubes is 0.01 ml and the largest volume of diluent in any individual tube is 9.99 L.