Can you help me design a forward primer using this sequence? 5´ATGACCATGATTACGGATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC 3´ and a reverse...
Can you help me design a forward primer using this
sequence?
5´ATGACCATGATTACGGATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC
3´
and a reverse primer with this sequence
´5-CGACTTCCAGTTCAACATCAGCCGCTACAGTCAACAGCAACTGATGGAAACCAGCCATCGCCATCTGCTGCACGCGGAAGAAGGCACATGGCTGAATATCGACGGTTTCCATATGGGGATTGGTGGCGACGACTCCTGGAGCCCGTCAGTATCGGCGGAATTCCAGCTGAGCGCCGGTCGCTACCATTACCAGTTGGTCTGGTGTCAAAAATAA-3´
Homework Answers
Forward primer -
5’GCCAGCTGGCG3’
reverse primer
5’ TTATTTTTGACA 3’
note- forward primer is added from the first base of 3’ end of the dna and the reverse primer is made from the last base 3’ end .
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Can you help me design a forward primer using this
sequence?
5´ATGACCATGATTACGGATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC
3´
and a reverse...
design a forward and reverse PCR primer based on underlined sequence in the following DNA sequence....
design a forward and reverse PCR primer based on underlined sequence in the following DNA sequence. 3' AAGCCAGGCCCTGGAGT......................TGGACTCGTGGGTGTG.....5' What does "PCR stand for and briefly describe PCR program
Forward primer 5'-ACCACCCAGCCCTTAGTGACCAGCTA-3' Reverse primer 5'-AAAGTTTTATTCATTGCAGAGGGGT-3' GAATTCTGTAATAACTATATAGAACTCTTCTTATATATGCTCAAATTTTA CATGCTAGCCTTCAGGTACATATCTTGGGTTGTTGGGTATTGTAGAAGAA TGTACTACAGGGCTTCAGCCCAGTTGACCAATGAGTGGGCTACGGGGTTT GTGAAAG
Forward primer 5'-ACCACCCAGCCCTTAGTGACCAGCTA-3' Reverse primer 5'-AAAGTTTTATTCATTGCAGAGGGGT-3' GAATTCTGTAATAACTATATAGAACTCTTCTTATATATGCTCAAATTTTA CATGCTAGCCTTCAGGTACATATCTTGGGTTGTTGGGTATTGTAGAAGAA TGTACTACAGGGCTTCAGCCCAGTTGACCAATGAGTGGGCTACGGGGTTT GTGAAAGGAGAGATGGAGAAGGAGGGACCATTAAGTACCTTGCTGCCTGA GTTCTGCTTTCCTCCTCCCTCTGAGGGTGAGCTGGGATCTCATCTGAGTT AAGGGCCCAGCTATCAATGGGAACTGTGAAACAGTCCAAGGGACATCAAT ATTAGGTCCCTAACAACTOCAGTTTCCTGGGGAATGATGTGGAAAATGCT CAGCCAAAGATGAAGAAGGTCTCACCTTCTGGGACAATGTCCCCTGCTOG GAACTGGTTCATCAGGCCATCTGGTCCCTTATTAAGACTATAATAACCCT AAGACTAAGTAGATGTGTTGATGTCCAATGAGTGCTTTCTGCAGACCTAG CACCAGGCAAGTGTTTGGAAACTGCAGCTTCAGCCCCTCTGGCCATCTGC CTACCCACCCCACCTGGAGACCTTAATGGGCCAAACAGCAAAGTCCAGGG GGCAGAGAGGAGGTACTTTOGACTATAAAGCTGGTGGGCATCCAGTAACC CCCAGCCCTTAGTGACCAGCTATAATCAGAGACCATCAGCAAGCAGGTAT GTACTCTCCTCTTTGGGCCTGGCTCCCCAGCCAAGACTCCAGOGACTTTA GGGAGAATGTGGGCTCCTCTCTTACATGGATCTTTTGCTAGCCTCAACCC TGCCTATCTTTCAGGTCATTGTTTCAACATGGCCCTGTTGGTGCACTTOC TACCCCTGCTGGCCCTGCTTGCCCTCTGGGAGCCCAAACCCACCCAGGCT TTTGTCAAACAGCATCTTTGTGGTCCCCACCTGGTAGAGGCTCTCTACCT GGTGTGTGGGGAGOGTGGCTTCTTCTACACACCCAAGTOCCGCOGTGAAG TGGAGGACCCACAAGTGGAACAACTGGAGCTGGGAGGAAGCCCOGGGGAC CTTCAGACCTTGGCGTTGGAGGTGGCCOGGCAGAAGCGTGGCATTGTGGA TCAGTGCTGCACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTACTGCA ACTAAGGCCCACCTCGACCOGCCCCACCCCTCTGCAATGAATAAAACTIT TGAATAAGCACCAAAAAAAAGAGTTCTATAATGAATGAAAAAGGATIGTG TATATAGACATCTTTTTCTCTGGCATTTATTGTCATGTTAGCATACTATT AAACCATTGTTAGGTTGGATGATTATATAATCATGTATGAAGCTTGTGAT AAAACACCAGGAATAATTCAAGTATCTGGAATTC 3 Question: 1) Predict the sizes of the PCR products. 2) Draw the DNA primers base pairing at genomic sequence.
Design the two primers (6 nucleotides), and add an EcoR1 cut site to the forward primer...
Design the two primers (6 nucleotides), and add an EcoR1 cut site to the forward primer (the 6-letter sequence), and BamH1 cut site to the reverse primer. Look up the cut site sequence. Label the restriction enzyme cut sites and add two overhang bases. Cut sites are included in the Tm. 5’-CTATGAGGTCCTGCGTTAGTGTTACC-3’ Forward: Tm: Reverse: Tm: Annealing temperature:
pls help ? Font Q10B. You would sequence a gene (5-CCGATTAAGGCTTAACTAACCGGTTAAGCG-3) with a reverse primer (5-CGCTTAACC-3)...
pls help ?
Font Q10B. You would sequence a gene (5-CCGATTAAGGCTTAACTAACCGGTTAAGCG-3) with a reverse primer (5-CGCTTAACC-3) with radioactive labeled chain terminator nucleotides. Fragments were run on a polyacrylamide gel to read a sequence from 5 3' of the gene sequence. Fill each lane on the gel with bands which terminate with either A, C, G, or I nucleotide. Also, mark the size of the fragments in lane L. Write the complete sequences as you read off the gel. (5 points)...
You would sequence a gene (5-CCGATTAAGGCTTAACTAACCGGTTAAGCG-3) with a reverse primer (5-CGCTTAACC-3) with radioactive labeled chain terminator...
You would sequence a gene (5-CCGATTAAGGCTTAACTAACCGGTTAAGCG-3) with a reverse primer (5-CGCTTAACC-3) with radioactive labeled chain terminator nucleotides. Fragments were run on a polyacrylamide gel to read a sequence from 5’-----------3’ of the gene sequence. Fill each lane on the gel with bands which terminate with either A, C, G, or T nucleotide. Also, mark the size of the fragments in lane L. Write the complete sequences as you read off the gel. A C G T L
please help me with both of these questions!!!! 3:33 PM Wed Mar 18 Enter answer... 5:06...
please help me with both of these questions!!!!
3:33 PM Wed Mar 18 Enter answer... 5:06 Exit You are trying to amplify an eye color gene in Drosophila. Based on the DNA sequence for the gene, you design the following primers: Forward Primer: 5' GCATGCTGAG CCTAGTAACT 3 Reverse Primer: 5' CTTAAAGCTT ACTGGTCAAC 3' True or false? These are good primers to use in PCR. Hint: use the formula: Tm (in C) = 4(G+C) + 2(A + T) True False ormula:...
You are given the following double-stranded DNA template (only top strand shown). Design a primer-pair to...
You are given the following double-stranded DNA template (only top strand shown). Design a primer-pair to amplify all of the red (ds) sequence, and only the red sequence? Primers should be 8 nts long (note: usually 17-25 nts long) Hint: Think about direction of DNA synthesis and annealing of primer to double-stranded template ! To answer, write the primer sequence (8 nts each) into the provided space below with the indicated 5' 3' polarity. 5'---AATGCCGTCAGCCGATCTGCCTCGAGTCAATC GATGCTGGTAACTTGGGGTATAAAGCTTACCCATGG TATCGTAGTTAGATTGATTGTTAGGTTCTTAGGTTTA GGTTTCTGGTATTGGTTTAGGGTCTTTGATGCTATTA ATTGTTTGGTTTTGATTTGGTCTTTATATGGTTTATG TTTTAAGCCGGGTTTTGTCTGGGATGGTTCGTCTGAT...
What is the sequence of the first forward primer sequence that you obtain? (You can copy...
What is the sequence of the first forward primer sequence that you obtain? (You can copy and paste it right from the website) I have a sequence: 1 catgacaact gaagcaaagt gcccttttct acatcccgcc ggcgctggcc gggcgctcca 61 ggaatggtgg cccgagcgcc tgaatctgca tatcctgcgc cagaacgctc cgttgtccga 121 tccgatggga gaggctttcg attatgcgaa ggcgtttaac agtctcgacc tcgcggcggt 181 caagaaggat ctggaagcgc tgatgaccga ttcgcagtcc tggtggccgg cggatttcgg 241 gcactacggc ccgttgttcg tccggatggc ctggcacgcg gcaggtacct accgcatcgg 301 cgatgggcgt ggcggtgccg gcgctggcca gcagcgtttc gcgcccacca acagctggcc 361 ggacaacgtc agtctggaca aggcacgcag gctcatctgg ccgatcaagc agaaatacgg 421 ccgcaagatc tcgtgggccg...
please help me!!!!!!!!! do both i need help Dz. You are creating PCR primers for the...
please help me!!!!!!!!!
do both i need help
Dz. You are creating PCR primers for the DNA sequence pictured. If the given sequence is the plus strand, what would be the reverse primer? (Type the primer in 5' to 3' orientation without spaces) 5' GTACTGCCAT TCGTAACTGT GTACTCAAGT AATGCCTGTC CATCATGTAA ATTGCATGGC CCTGATTGGA TATGCCAAAG GCTTTTGCAA GTCCCCATAG GTACTGGACA GTAGTACGTT GTCCTGAGGC GCGCTATAGG GTCGAAACTG 3 Enter answer. D8 You are creating PCR primers for the DNA sequence pictured. If the given sequence is the plus...
True or false? The forward primer attaches to the 5 prime end of the minus(-) strand....
True or false? The forward primer attaches to the 5 prime end of the minus(-) strand. True or false? The reverse primer attaches to the 3 prime end of the plus(+) strand. Can you explain why as well im not fully understanding this, thank you.
Forward primer 5'-ACCACCCAGCCCTTAGTGACCAGCTA-3' Reverse primer 5'-AAAGTTTTATTCATTGCAGAGGGGT-3' GAATTCTGTAATAACTATATAGAACTCTTCTTATATATGCTCAAATTTTA CATGCTAGCCTTCAGGTACATATCTTGGGTTGTTGGGTATTGTAGAAGAA TGTACTACAGGGCTTCAGCCCAGTTGACCAATGAGTGGGCTACGGGGTTT GTGAAAGGAGAGATGGAGAAGGAGGGACCATTAAGTACCTTGCTGCCTGA GTTCTGCTTTCCTCCTCCCTCTGAGGGTGAGCTGGGATCTCATCTGAGTT AAGGGCCCAGCTATCAATGGGAACTGTGAAACAGTCCAAGGGACATCAAT ATTAGGTCCCTAACAACTOCAGTTTCCTGGGGAATGATGTGGAAAATGCT CAGCCAAAGATGAAGAAGGTCTCACCTTCTGGGACAATGTCCCCTGCTOG GAACTGGTTCATCAGGCCATCTGGTCCCTTATTAAGACTATAATAACCCT AAGACTAAGTAGATGTGTTGATGTCCAATGAGTGCTTTCTGCAGACCTAG CACCAGGCAAGTGTTTGGAAACTGCAGCTTCAGCCCCTCTGGCCATCTGC CTACCCACCCCACCTGGAGACCTTAATGGGCCAAACAGCAAAGTCCAGGG GGCAGAGAGGAGGTACTTTOGACTATAAAGCTGGTGGGCATCCAGTAACC CCCAGCCCTTAGTGACCAGCTATAATCAGAGACCATCAGCAAGCAGGTAT GTACTCTCCTCTTTGGGCCTGGCTCCCCAGCCAAGACTCCAGOGACTTTA GGGAGAATGTGGGCTCCTCTCTTACATGGATCTTTTGCTAGCCTCAACCC TGCCTATCTTTCAGGTCATTGTTTCAACATGGCCCTGTTGGTGCACTTOC TACCCCTGCTGGCCCTGCTTGCCCTCTGGGAGCCCAAACCCACCCAGGCT TTTGTCAAACAGCATCTTTGTGGTCCCCACCTGGTAGAGGCTCTCTACCT GGTGTGTGGGGAGOGTGGCTTCTTCTACACACCCAAGTOCCGCOGTGAAG TGGAGGACCCACAAGTGGAACAACTGGAGCTGGGAGGAAGCCCOGGGGAC CTTCAGACCTTGGCGTTGGAGGTGGCCOGGCAGAAGCGTGGCATTGTGGA TCAGTGCTGCACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTACTGCA ACTAAGGCCCACCTCGACCOGCCCCACCCCTCTGCAATGAATAAAACTIT TGAATAAGCACCAAAAAAAAGAGTTCTATAATGAATGAAAAAGGATIGTG TATATAGACATCTTTTTCTCTGGCATTTATTGTCATGTTAGCATACTATT AAACCATTGTTAGGTTGGATGATTATATAATCATGTATGAAGCTTGTGAT AAAACACCAGGAATAATTCAAGTATCTGGAATTC 3 Question: 1) Predict the sizes of the PCR products. 2) Draw the DNA primers base pairing at genomic sequence.
pls help ?
Font Q10B. You would sequence a gene (5-CCGATTAAGGCTTAACTAACCGGTTAAGCG-3) with a reverse primer (5-CGCTTAACC-3) with radioactive labeled chain terminator nucleotides. Fragments were run on a polyacrylamide gel to read a sequence from 5 3' of the gene sequence. Fill each lane on the gel with bands which terminate with either A, C, G, or I nucleotide. Also, mark the size of the fragments in lane L. Write the complete sequences as you read off the gel. (5 points)...
please help me with both of these questions!!!!
3:33 PM Wed Mar 18 Enter answer... 5:06 Exit You are trying to amplify an eye color gene in Drosophila. Based on the DNA sequence for the gene, you design the following primers: Forward Primer: 5' GCATGCTGAG CCTAGTAACT 3 Reverse Primer: 5' CTTAAAGCTT ACTGGTCAAC 3' True or false? These are good primers to use in PCR. Hint: use the formula: Tm (in C) = 4(G+C) + 2(A + T) True False ormula:...
You are given the following double-stranded DNA template (only top strand shown). Design a primer-pair to amplify all of the red (ds) sequence, and only the red sequence? Primers should be 8 nts long (note: usually 17-25 nts long) Hint: Think about direction of DNA synthesis and annealing of primer to double-stranded template ! To answer, write the primer sequence (8 nts each) into the provided space below with the indicated 5' 3' polarity. 5'---AATGCCGTCAGCCGATCTGCCTCGAGTCAATC GATGCTGGTAACTTGGGGTATAAAGCTTACCCATGG TATCGTAGTTAGATTGATTGTTAGGTTCTTAGGTTTA GGTTTCTGGTATTGGTTTAGGGTCTTTGATGCTATTA ATTGTTTGGTTTTGATTTGGTCTTTATATGGTTTATG TTTTAAGCCGGGTTTTGTCTGGGATGGTTCGTCTGAT...
please help me!!!!!!!!!
do both i need help
Dz. You are creating PCR primers for the DNA sequence pictured. If the given sequence is the plus strand, what would be the reverse primer? (Type the primer in 5' to 3' orientation without spaces) 5' GTACTGCCAT TCGTAACTGT GTACTCAAGT AATGCCTGTC CATCATGTAA ATTGCATGGC CCTGATTGGA TATGCCAAAG GCTTTTGCAA GTCCCCATAG GTACTGGACA GTAGTACGTT GTCCTGAGGC GCGCTATAGG GTCGAAACTG 3 Enter answer. D8 You are creating PCR primers for the DNA sequence pictured. If the given sequence is the plus...
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