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You would sequence a gene (5-CCGATTAAGGCTTAACTAACCGGTTAAGCG-3) with a reverse primer (5-CGCTTAACC-3) with radioactive labeled chain terminator...

You would sequence a gene (5-CCGATTAAGGCTTAACTAACCGGTTAAGCG-3) with a reverse primer (5-CGCTTAACC-3) with radioactive labeled chain terminator nucleotides. Fragments were run on a polyacrylamide gel to read a sequence from 5’-----------3’ of the gene sequence. Fill each lane on the gel with bands which terminate with either A, C, G, or T nucleotide. Also, mark the size of the fragments in lane L. Write the complete sequences as you read off the gel.

A C G T L

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Answer #1

A C G TL 31 = 3-GCGAATTGGCCAATCAATTCGGAATTAGCC-5 - Template 5-CGCTTAACC.GGTTAGTTAAGCCTTAATCGG-3 - Primer PRIMER 21 2x dy A

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