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Font Q10B. You would sequence a gene (5-CCGATTAAGGCTTAACTAACCGGTTAAGCG-3) with a reverse primer (5-CGCTTAACC-3)...
You would sequence a gene (5-CCGATTAAGGCTTAACTAACCGGTTAAGCG-3) with a reverse primer (5-CGCTTAACC-3) with radioactive labeled chain terminator...
You would sequence a gene (5-CCGATTAAGGCTTAACTAACCGGTTAAGCG-3) with a reverse primer (5-CGCTTAACC-3) with radioactive labeled chain terminator nucleotides. Fragments were run on a polyacrylamide gel to read a sequence from 5’-----------3’ of the gene sequence. Fill each lane on the gel with bands which terminate with either A, C, G, or T nucleotide. Also, mark the size of the fragments in lane L. Write the complete sequences as you read off the gel. A C G T L
Can you help me design a forward primer using this sequence? 5´ATGACCATGATTACGGATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC 3´ and a reverse...
Can you help me design a forward primer using this sequence? 5´ATGACCATGATTACGGATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGC 3´ and a reverse primer with this sequence ´5-CGACTTCCAGTTCAACATCAGCCGCTACAGTCAACAGCAACTGATGGAAACCAGCCATCGCCATCTGCTGCACGCGGAAGAAGGCACATGGCTGAATATCGACGGTTTCCATATGGGGATTGGTGGCGACGACTCCTGGAGCCCGTCAGTATCGGCGGAATTCCAGCTGAGCGCCGGTCGCTACCATTACCAGTTGGTCTGGTGTCAAAAATAA-3´
Suppose you want to sequence the following DNA segment: 5’-GATCTCTTGAAATG-primer site-3’ You clone the fragment into...
Suppose you want to sequence the following DNA segment: 5’-GATCTCTTGAAATG-primer site-3’ You clone the fragment into a plasmid, transform it into bacterial cells and isolate DNA from one of the bacterial colonies. You use the Sanger sequencing method to determine the sequence, separating the products from the sequencing reactions by gel electrophoresis. Draw the bands that should appear on the gel from the four sequencing reactions.
The following diagram represents a replication bubble associated with DNA synthesis. Based on this diagram, select...
The following diagram represents a replication bubble associated
with DNA synthesis. Based on this diagram, select all of the
options below that are true.
1 | 2 ---- Quadrants 1 and 4 are associated with lagging strand synthesis Quadrants 1 and 4 are associated with leading strand synthesis Quadrants 1 and 2 are associated with lagging strand synthesis Quadrants 3 and 4 are associated with lagging strand synthesis Synthesis of both daughter strands is completely continuous Telomerase activity is needed...
Can you help me with this question? 7. On Day 4, you will obtain your sequence....
Can you help me with this question?
7. On Day 4, you will obtain your sequence. An old technology would run chain-terminated products on a polyacrylamide gel to identify the fragments. Let's say you ran 4 reactions. Each included all of the dNTPs and one of the four ddNTPs. On the figure below, draw the gel that would result from the following template sequence (make sure to pay attention to the 5' and 3 ends. Remember how DNA synthesis works!):...
Write true or false ______ 1. The DNA sequence of one human being is on average...
Write true or false ______ 1. The DNA sequence of one human being is on average 99.9% identical to another random human being. ______ 2. As of 2009, all living human beings have had their entire genome sequenced. ______ 3. The nucleotide bases present in a DNA sequence are A, U, G, C. ______ 4. Techniques that enabled scientists to clone genes were developed in the 1970s. ______ 5. A restriction enzyme is useful because it is a generic enzyme...
6. Using the answers to questions 2-5 and the below DNA sequence, predict the mRNA sequence,...
6. Using the answers to questions 2-5 and the below DNA sequence, predict the mRNA sequence, the tRNA anticodons, and the amino acid sequences (use the three letter code) that would result from it. (3 points) DNA +1 15'GICIA I G C A A CICATI I AA GG 3' 3" CA GATA C GTIGA GIA A A IICC 5 mRNA tRNA anticodons amino acids 7. You are interested in a gene that codes for a 20 amino acid-long protein. (1.5...
please help, thank you! The DNA sequence below represents an edited version of the PISTILLATA gene...
please help, thank you!
The DNA sequence below represents an edited version of the PISTILLATA gene from the model plant Arabidopsis thaliana. The sequence comes from the nontemplate (coding) strand. Use the sequence to answer all questions on this assignment. 9 5'-TTTCTCTCTCTATCTCATCAATGTTACTTTAAAACCAATTTACCTTAATAGGTAAGCTCCTCTTCTTG TTCTTCATATAAATCCACATATCCTCTCCTCCATATCTTAACAATTTCATAGCAAACCCTAAAATTGAGA AAGAGATAGAGAGAGAAAGATGGGTAGAGGAAAGATCGAGATAAAGAGGATAGAGAACGCAAACAA CAGAGTGGTGACGGTATCCTTGCTCTCTTTTTAATCTAAAGTTTCTTTGTCTCAAAAATATTCCATGGC CAAAATCATGTAATAAGTTTTCAATTTCTTTACCAAAAACATGTCAAAAGACCCTTGAATCTTTAACCCT AGATGCAGATAACAACAGGAGATGGGATGATGAGAGATCATGACTCGATTGATCATCGAGATTTTAT AATCTCATCCTGATCAACTCCTATCTATAATATCGTGGTCTTTAGTTTGTCTTTATCAATCTGTGTGTCT TAATCTCAATAAAATATACTCATTAAAATCAAAATCCATAAATACGGACATAAACACGACAGACTTACG -3 0 . What kind of gene features would you expect Arabidopsis thaliana to have, bacterial or eukaryotic? 2. The gene contains a sequence that is recognized by the AP1 transcription...
3.13 pts) The restriction enzyme known as Notl recognizes the following sequence: 5-GCGGCCGC-3 3-CGCCGGCG-5 However, if...
3.13 pts) The restriction enzyme known as Notl recognizes the following sequence: 5-GCGGCCGC-3 3-CGCCGGCG-5 However, if the cytosines in this sequence have been methylated. NotI will not cleave the DNA at this site. For this reason, Net is commonly used to investigate the methylation state of CpG islands. A researcher has studied a gene, which we will call T. that is found in com, and encodes a transporter involved in the uptake of phosphate from the soil. A CpG island...
pls help The DNA sequence below is 300 bases long. This is only one strand of...
pls help
The DNA sequence below is 300 bases long. This is only one strand of DNA going from 5' starting at base 1081 to 3' ending at base 1380. The complementary strand is NOT shown. The sequence is broken up into 10 base sections to make counting easier. Design primers to amplify a DNA fragment that is 150bps in length. 1081 cagtatcagg tggtggcccc ttgcccccag tcagcaccct gacatcactg cacagtctgt 1141 ctgcctcgcc tgctccccac catggactca toatgacctc cctgcccagc gtcatgagtc 1201 tgggagagtc ctctctcctc ataggtcaaa ccgtacctgt...
The following diagram represents a replication bubble associated
with DNA synthesis. Based on this diagram, select all of the
options below that are true.
1 | 2 ---- Quadrants 1 and 4 are associated with lagging strand synthesis Quadrants 1 and 4 are associated with leading strand synthesis Quadrants 1 and 2 are associated with lagging strand synthesis Quadrants 3 and 4 are associated with lagging strand synthesis Synthesis of both daughter strands is completely continuous Telomerase activity is needed...
Can you help me with this question?
7. On Day 4, you will obtain your sequence. An old technology would run chain-terminated products on a polyacrylamide gel to identify the fragments. Let's say you ran 4 reactions. Each included all of the dNTPs and one of the four ddNTPs. On the figure below, draw the gel that would result from the following template sequence (make sure to pay attention to the 5' and 3 ends. Remember how DNA synthesis works!):...
6. Using the answers to questions 2-5 and the below DNA sequence, predict the mRNA sequence, the tRNA anticodons, and the amino acid sequences (use the three letter code) that would result from it. (3 points) DNA +1 15'GICIA I G C A A CICATI I AA GG 3' 3" CA GATA C GTIGA GIA A A IICC 5 mRNA tRNA anticodons amino acids 7. You are interested in a gene that codes for a 20 amino acid-long protein. (1.5...
please help, thank you!
The DNA sequence below represents an edited version of the PISTILLATA gene from the model plant Arabidopsis thaliana. The sequence comes from the nontemplate (coding) strand. Use the sequence to answer all questions on this assignment. 9 5'-TTTCTCTCTCTATCTCATCAATGTTACTTTAAAACCAATTTACCTTAATAGGTAAGCTCCTCTTCTTG TTCTTCATATAAATCCACATATCCTCTCCTCCATATCTTAACAATTTCATAGCAAACCCTAAAATTGAGA AAGAGATAGAGAGAGAAAGATGGGTAGAGGAAAGATCGAGATAAAGAGGATAGAGAACGCAAACAA CAGAGTGGTGACGGTATCCTTGCTCTCTTTTTAATCTAAAGTTTCTTTGTCTCAAAAATATTCCATGGC CAAAATCATGTAATAAGTTTTCAATTTCTTTACCAAAAACATGTCAAAAGACCCTTGAATCTTTAACCCT AGATGCAGATAACAACAGGAGATGGGATGATGAGAGATCATGACTCGATTGATCATCGAGATTTTAT AATCTCATCCTGATCAACTCCTATCTATAATATCGTGGTCTTTAGTTTGTCTTTATCAATCTGTGTGTCT TAATCTCAATAAAATATACTCATTAAAATCAAAATCCATAAATACGGACATAAACACGACAGACTTACG -3 0 . What kind of gene features would you expect Arabidopsis thaliana to have, bacterial or eukaryotic? 2. The gene contains a sequence that is recognized by the AP1 transcription...
3.13 pts) The restriction enzyme known as Notl recognizes the following sequence: 5-GCGGCCGC-3 3-CGCCGGCG-5 However, if the cytosines in this sequence have been methylated. NotI will not cleave the DNA at this site. For this reason, Net is commonly used to investigate the methylation state of CpG islands. A researcher has studied a gene, which we will call T. that is found in com, and encodes a transporter involved in the uptake of phosphate from the soil. A CpG island...
pls help
The DNA sequence below is 300 bases long. This is only one strand of DNA going from 5' starting at base 1081 to 3' ending at base 1380. The complementary strand is NOT shown. The sequence is broken up into 10 base sections to make counting easier. Design primers to amplify a DNA fragment that is 150bps in length. 1081 cagtatcagg tggtggcccc ttgcccccag tcagcaccct gacatcactg cacagtctgt 1141 ctgcctcgcc tgctccccac catggactca toatgacctc cctgcccagc gtcatgagtc 1201 tgggagagtc ctctctcctc ataggtcaaa ccgtacctgt...
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