Use this information to construct a restriction map of a circular plasmid. NdeI 10kb ...
Use this information to construct a restriction map of a circular plasmid.
NdeI 10kb NdeI/EcoRI 1kb, 2kb, 7kb
PstI 10kb EcoRI/PstI 2kb, 3kb, 5kb
EcoRI 3kb, 7kb NdeI/PstI 3kb, 7kb
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Use this information to construct a restriction map of a
circular plasmid.
NdeI 10kb
...
You digest a 10Kb Linear ECoRI dna fragment with TWO restriction enzymes and obtain the following...
You digest a 10Kb Linear ECoRI dna fragment with TWO restriction enzymes and obtain the following data Hind iii..... 3kb, 7kb BamHi ... 2 kb and 8kb HInd iii + bamHI ..... 1kb, 2kb, 7kb Draw a restriction map of this DNA fragment, labeling the sites for EcoRI, bahHI, and HIndiii
I need help making a restriction digest map for both of these sets of fragments. Please...
I need help making a
restriction digest map for both of these sets of fragments. Please
explain the steps and include drawing.
Draw restriction maps for the plasmids that produced the following fragments from restriction digestion: Digest Performed Size of DNA fragments produced BamHI BamHI/HindI 2.5kb & 1.5kb BamHI/EcoRI 3kb & 1kb HindIlI/EcoRI 3.5kb &0.5kb 4kp Digest Performed Size of DNA fragments produced BamHI BamHI & Sal1 4kb, 5kb BamHI & Pst1 6kb& 3kb Sal1&Pst1 1kb& 8kb kb
5. You have a linear DNA fragment and you wish to generate a restriction map using...
5. You have a linear DNA fragment and you wish to generate a restriction map using Mstl and EcoR1. When the framgent is digested with Mst1 and the DNA is run on a gel you observe a 4kb and a 6kb fragment. When the DNA is digested with EcoR1, 1kb, 4kb and 5kb fragments are generated, A double digest using both enzymes produce 1kb, 2kb, 3kb and 4kb fragments. Explain these results and draw a restirction map consistent with these...
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb)....
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb). Using this map as a guide, give the number of restriction fragments along with their associated lengths that would result from digesting PGEN101 with the restriction enzymes EcoRI, BamHII, anda combination of EcoRI + BamHI. BamHI BamHI BamHI / PGEN101 (20 kb) Mb EcoRI Digest Performed Size Emments Obtained EcoRI........ BamHI.. EcoRI + BamHI.... Two freshmen college students, interested in becoming gene jocks, performed...
construct a restriction of a circular pvn plasmid, using the following data yor map should indicale,...
construct a restriction of a circular pvn plasmid, using the following data yor map should indicale, either the relative positions of each restriction site or give the distances between the restriction J DNA sizes of fragments ( bp) uncut DNA 112000 PNA cut with sma I 12000 DNA cut with Hind III 2000 PNA cut with ECORI 12000 onn cut with sma I FEcori 7000, 5000 ana cut with SMO I + HINDIII 10.000, 2000 min cut with Hind II...
Practice Problem 1 A restriction map for a fictitious 4000 base pair plasmid is shown in...
Practice Problem 1 A restriction map for a fictitious 4000 base pair plasmid is shown in Figure 5. Create a figure predicting the appearance of a gel showing digests with EcoRI, BamHI, and EcoRI and BamHI combined. Your figure should include a lane showing a 1kbladder for reference. (A photo of a 1kb ladder is provided in Figure 3A.) > > FOORIOL ULO BamHI (620) 325 BamHI (3090) Abel 23 Plasmid 4000bp BamHI (945) 2145 Figure 5. A restriction map...
Calculate the restriction map of plasmid JLB51given the following restriction digest data. HindIII 3.82 0.18 kb...
Calculate the restriction map of plasmid JLB51given the following restriction digest data. HindIII 3.82 0.18 kb BamHI 2.35, 1.65 kb EcoRI 3.00, 1.0 kb HindIII, BamHI 2.35, 1.2, 0.27, 0.18 kb HindIII, EcoRI 1.87, 1.00, 0.95, 0.18 kb BamHI, EcoRI 1.6, 1.4, 0.75, 0.25 kb draw the circular map
Now determine the sizes of the fragments generated after digesting the r-plasmid with each of the...
Now determine the sizes of the fragments generated after digesting the r-plasmid with each of the restriction enzymes or combination of restriction enzymes. r-plasmid digested with Pstl r-plasmid digested with EcoRI r-plasmid digested with both Pstl and EcoRI Question 8. (3 pts.) Use this information to determine the relative placement of these restriction sites in the r-plasmid, and then draw restriction site maps of the vector and the r-plasmid on the following circular chromosome templates. The map should show the...
A restriction digest of a circular plasmid is conducted using two different restriction enzymes. Restriction enzyme...
A restriction digest of a circular plasmid is conducted using two different restriction enzymes. Restriction enzyme 1 cuts the plasmid in one place while restriction enzyme 2 cuts in two places. All restriction sites are at least 1kB apart from each other. Based on this information, the resulting gel should have _____ bands for the enzyme 1 reaction, _____ bands for the enzyme 2 reaction, and _____ bands for the reaction that contains both enzymes A 1; 2; 3 B...
Use the results of the agarose gel electrophoresis separation to construct a restriction map for the...
Use the results of the agarose gel electrophoresis separation to
construct a restriction map for the sample of DNA
EcoRI Control EcoRI Pst Pst 15kb-. 8kb → 3 kb
I need help making a
restriction digest map for both of these sets of fragments. Please
explain the steps and include drawing.
Draw restriction maps for the plasmids that produced the following fragments from restriction digestion: Digest Performed Size of DNA fragments produced BamHI BamHI/HindI 2.5kb & 1.5kb BamHI/EcoRI 3kb & 1kb HindIlI/EcoRI 3.5kb &0.5kb 4kp Digest Performed Size of DNA fragments produced BamHI BamHI & Sal1 4kb, 5kb BamHI & Pst1 6kb& 3kb Sal1&Pst1 1kb& 8kb kb
5. You have a linear DNA fragment and you wish to generate a restriction map using Mstl and EcoR1. When the framgent is digested with Mst1 and the DNA is run on a gel you observe a 4kb and a 6kb fragment. When the DNA is digested with EcoR1, 1kb, 4kb and 5kb fragments are generated, A double digest using both enzymes produce 1kb, 2kb, 3kb and 4kb fragments. Explain these results and draw a restirction map consistent with these...
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb). Using this map as a guide, give the number of restriction fragments along with their associated lengths that would result from digesting PGEN101 with the restriction enzymes EcoRI, BamHII, anda combination of EcoRI + BamHI. BamHI BamHI BamHI / PGEN101 (20 kb) Mb EcoRI Digest Performed Size Emments Obtained EcoRI........ BamHI.. EcoRI + BamHI.... Two freshmen college students, interested in becoming gene jocks, performed...
construct a restriction of a circular pvn plasmid, using the following data yor map should indicale, either the relative positions of each restriction site or give the distances between the restriction J DNA sizes of fragments ( bp) uncut DNA 112000 PNA cut with sma I 12000 DNA cut with Hind III 2000 PNA cut with ECORI 12000 onn cut with sma I FEcori 7000, 5000 ana cut with SMO I + HINDIII 10.000, 2000 min cut with Hind II...
Practice Problem 1 A restriction map for a fictitious 4000 base pair plasmid is shown in Figure 5. Create a figure predicting the appearance of a gel showing digests with EcoRI, BamHI, and EcoRI and BamHI combined. Your figure should include a lane showing a 1kbladder for reference. (A photo of a 1kb ladder is provided in Figure 3A.) > > FOORIOL ULO BamHI (620) 325 BamHI (3090) Abel 23 Plasmid 4000bp BamHI (945) 2145 Figure 5. A restriction map...
Calculate the restriction map of plasmid JLB51given the following restriction digest data. HindIII 3.82 0.18 kb BamHI 2.35, 1.65 kb EcoRI 3.00, 1.0 kb HindIII, BamHI 2.35, 1.2, 0.27, 0.18 kb HindIII, EcoRI 1.87, 1.00, 0.95, 0.18 kb BamHI, EcoRI 1.6, 1.4, 0.75, 0.25 kb draw the circular map
Now determine the sizes of the fragments generated after digesting the r-plasmid with each of the restriction enzymes or combination of restriction enzymes. r-plasmid digested with Pstl r-plasmid digested with EcoRI r-plasmid digested with both Pstl and EcoRI Question 8. (3 pts.) Use this information to determine the relative placement of these restriction sites in the r-plasmid, and then draw restriction site maps of the vector and the r-plasmid on the following circular chromosome templates. The map should show the...
Use the results of the agarose gel electrophoresis separation to
construct a restriction map for the sample of DNA
EcoRI Control EcoRI Pst Pst 15kb-. 8kb → 3 kb
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