Describe fluorescence correlation spectroscopy and how it is used to study protein structure.
Describe fluorescence correlation spectroscopy and how it is used to study protein structure.
d. BC e. a and b f. None of the above 14) Briefly, describe whether you would expect the change in Trp fluorescence intensity to be greater for a protein-protein binding interaction or for protein folding/unfolding and justify your answer. 2 pts 15 Briefly, describe why CD spectroscopy would be a superior technique for analyzing the structure of the following protein sequence compared to UV Nis spectroscopy. 2 pts SEQUENCE: LAVISREPPIHGGSQCMNNHIPPKLFF
Questions in photo. From the Green Synthesis and Study of a
Fluorescent Natural Product experiment.
Fluorescence Data: Describe the observed under the UV light in the test tube and on the filter paper. (e.g. color, brightness etc. fluorescent activity of each test tube of the 4-methylumbelliferone i ) Draw the structure of 4- erone under the neutral, acidic and basic conditions. For the acidic and basic conditions drawa methylumbellif resonance structure to depict a new chromophore likely responsible for its...
6) Proteins are composed of amino acids polymerized into long chains. The structure of a protein - that is, its overall shape and how the chains are "folded” around each other - is very important for its function. In an aqueous environment, in an active, folded state the hydrophilic amino acids in the protein are facing outwards exposed to the water and the hydrophobic amino acids are hidden away from the water in the core of the protein. In a...
Describe how IR spectroscopy might be used to monitor the progress of the following reaction: H2Cr04 OH OH H20, H2S04 1-butanol butanoic acid
a) Fluorescent proteins such as green fluorescent protein (GFP) are commonly used in microscopy experiments. Describing the process of fluorescence, what is GFP and what is the main benefit of using fluorescent proteins over other fluorophores? b) Describe how fluorescent proteins can be used to (i) study a protein’s gene expression and (ii) localise proteins of interest within cells? c) The resolution of a standard confocal microscope is approximately 200nm. Describe an experiment that can be used to show whether...
Describe how IR spectroscopy could be used to determine which of two compounds has stronger hydrogen bonding.
Define hydropathy and how that can be used to determine protein structure.
List and describe the two approaches to determining the tertiary structure of a protein. Experimental Determination Computational prediction There are three methods of computational structure prediction. Define each of the methods and describe when each method would be used. There are three types of helices formed by the secondary structure of protein. List three helices and rate how tightly coiled they are. Explain your reasoning. Choose any three of the twenty amino acids. Based on what you know about the...
Can you describe the research methods that are used to study brain structure and function?
Describe using spectroscopy why benzaldehyde is used in sunscreen