Two peptone iron deeps were inoculated with bacteria X and bacteria Y respectively. After 24 hours,...
Two peptone iron deeps were inoculated with bacteria X and bacteria Y respectively. After 24 hours, both test tubes show growth and the tube with bacteria X shows a black precipitate in the bottom of the tube. The tube with Bacteria Y shows no change in color. Identify the black precipitate and explain how it forms (where did it come from)? (be specific and explain the reaction)
Homework Answers
Solution-
For the detection of hydrogen sulfide produced by the
microorganisms Pentane Agar Dip is used.
Sodium Triosulphate and ferric ammonia citrate is ammonium citrate
are its constituents.
Sulphide are produced when the baterial enzymes which is present in
(x) reacts with Sodium triosupate.
H2S is fored when sulphide reats with hydrogen ion ,then its
reaction with ferric ions from the ferric ammonium citrate and
produces insoluble heavy metal sulphides and it appears as black
precipitate.
Hence the bacteria x is H2S PRODUCING BACTERIA.
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Two peptone iron deeps were inoculated with bacteria X and
bacteria Y respectively. After 24 hours,...
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Use the Broch Care Putter (fig. 4) chart to fill in the datatable Table 3. Hroth...
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4. Both high and low temperatures reduced the amount of color
(product) produced. However, the cause of the reduction is
different in the two cases. Explain what the differences are.
me. The wa Activity 2: temperature In this activity, you will examine the effect of different temperatures on the activity of the enzyme. and enzyme mixture must come to a specific tempera yme mixture must come to a specific temperature before the addition of the substrate. 1. Write your hypothesis...
Background
Background continued...
Questions
Experiment 2: TSI The triple sugar iron agar (TSI) can identify gram negative bacteria based on how they metabolize carbohydrates. It contains three carbohydrates-glucose, lactose and sucrose, in addition to sodium thiosulfate which some bacteria use in the production of hydrogen sulfide (H2S). It also contains iron in the ferrous form and phenol red as a pH indicator. TSI is poured into a tube when heated to a liquid form, and the tube is slanted as...
please help me. answer only first page. thank you!
p. If you accidentally forgot to inoculate your organism in an OF-glucose tube with oil, and only had one yellow “no oil” tube, what type of carbohydrate catabolism would describe that organism (fermenter, oxidative, neither)? Explain your answer. q. What are two other results from the OF-glucose tubes other than their type of carbohydrate catabolism? r. How can organisms that don't use starch grow on a starch agar plate? Carbohydrate Catabolism...
Lab Report-Carbohydrates 1. Purpose 2. Special Media for Isolating Bacteria (Lab #12) a. Why are dyes such as phenol red, eosin or methylene blue added to the media? b. How does the bacterium change the media (i.e color of agar or colonies) after incubation? C. In this experiment, which media are selective, and which are differential? d. How did the results observe on the mannitol salt agar and EMB agar correlate to the Gram reaction of the bacteria? e. What...
d. Suppose you mistakenly acidify the filtrate with HCl instead of with HNO3. What would you obser after completing the halide test, and what would that observation lead you to conclude? Organic Laboratory Technique Primer Sodium Fusions and lon Testing Background: The elements that commonly occur in organic compounds along with carbon, hydrogen, and oxygen, are: nitrogen sulfur, and the halogens. The detection of these latter three elements depends on converting them into water soluble lons (CNS , and X...
Use the Broch Care Putter (fig. 4) chart to fill in the datatable Table 3. Hroth Liquid Culture Growth Patterns (Y/N) Environmental Samples Lab Hedy Sampler Belybutton Sink Turbidity Results: Use the Colony Description (fig. 3) chart to fill in the data tables. Use a new line for each different colony type found on the environmental and body part samples. Table 1: Environmental sample Colony Descriptions Whole colony Margin Elevation Pigment appearance Area sampled Lab sink HH 217 Incubated at...
microbiology help
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