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After digesting PKB200 and PKB201 plasmids you are expected to see two bands on the gel....

After digesting PKB200 and PKB201 plasmids you are expected to see two bands on the gel. However, if you see only one band, what could be possible reasons??

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Restriction endo nuclease digest the plasmid at a specific points of recognition called restriction site.Different plasmid have diffrent restriction sites .Here we got only one band istead of two band as we cutted through two restriction site. Normally scientsits use 2restriction endonuclease enzyme for cutting instead of one. Here that maybe one reason for getting one reason another may be mutation happened in plasmid.

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