Question

For the unknown sequence shown below,

(A) Fnd its corresponding gene in the NCBI Gene database, (B) find one protein sequence expressed from the gene using the RefSeq database, (C) find the splicing structure of this gene using UCSC genome browser, (D) find its expression pattern across different human tissues using Expression Atlas.

>unknown.seq

TCAGAGGGAGCAGACACATTTAAATCCTCCTTGTCCTAATTGGCTATGTTCCTAACTTGT                 TTTCTATCACTACAGTGAATGCTGCAATACTGATATAAGAAAAAATAAAATAAAATAGTA                 ACCTCTGCTTCAATGTACAGTTTCCAGAATCTGCCAGAACTGGGGAACTGGGCAACAAGG                 CGTTCATAAGTCTTCCGTGCTTTGTCTATAGGTTGATTCTAAAATTGAAAACCAATAAAC                 AGCATTTACAATGTTAGGATTATGAAAATATTATTCACTGCAGAACCAAGTAGTGTGATT                 GGACCCATAGAGAAGGAAATGTAATCCTATATCACTAAACCTGTGCCTCTCGAATGAGAA                 TGCTCCAAGCATCAAGGTCATATGGATTCTCTTCTAATTTCTTTTCCGCTTTCTTCA

Answer 1

Ans:

(A)  The corresponding gene in the NCBI Gene database is;

Human DNA sequence from clone RP1-85M6 on chromosome 11p12-13 Contains the CSTF3 gene for cleavage stimulation factor, 3' pre-RNA, subunit 3, 77kD, PIGCP1 pseudogene (phosphatidylinositol glycan, class C), the gene for a novel protein similar to testis-specific protein PBS13, the gene for a novel 58.3 KDA protein, ESTs, STSs, GSSs and CpG islands

(B) protein sequence expressed from the gene using the RefSeq database

cleavage stimulation factor subunit 3 isoform 2 [Homo sapiens]

(C)