Some mutations occur in the promoter of Msh2 and decrease its expression relative to wild-type cells...
Some mutations occur in the promoter of Msh2 and decrease its expression relative to wild-type cells leading to cancer. Would you confirm this pattern using DNA sequence or RNA sequence and why?
Homework Answers
DNA sequence.
The promoter is present upstream of the transcription start site. So it is not transcribed ding the transcription.
If we want to confirm that there is a mutation in the promoter then we need to perform DNA sequencing.
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Some mutations occur in the promoter of Msh2 and decrease its
expression relative to wild-type cells...
Shown below are the amino acid sequences of the wild-type and three mutant forms of a...
Shown below are the amino acid sequences of the wild-type and three mutant forms of a short protein. Each mutation results from a single nucleotide change (transition/transversion / insertion / deletion). Use this information to answer the following questions. Hint: First, reconstruct as much as you can of the wild-type RNA sequence and then reference that sequence when analyzing the mutations. Wild type: met - gin-ala - ser-val - arg - phe Mutant 1: met - gln - pro-ser -...
Shown below are the amino acid sequences of the wild-type and three mutant forms of a...
Shown below are the amino acid sequences of the wild-type and three mutant forms of a short protein. Each mutation results from a single nucleotide change (transition / transversion / insertion / deletion). Use this information to answer the following questions. Hint: First, reconstruct as much as you can of the wild-type RNA sequence and then reference that sequence when analyzing the mutations. Wild type: met – gln – ala – ser – val – arg – phe Mutant 1:...
Question 10 A human chromosome has 3 origins of replication. Assuming that all 3 origins of...
Question 10 A human chromosome has 3 origins of replication. Assuming that all 3 origins of replication are used at the same time, what is the maximum number of helicases present on DNA during DNA replication (not including DNA repair)? 12 O 3 O2 Question 11 Which of the following correctly compares primase and telomerase? O Both primase and telomerase synthesize DNA using a DNA template. O Primase synthesizes RNA using an RNA template, but telomerase synthesizes DNA using a...
What control elements regulate expression of the mPGES-1 gene? The promoter of a gene includes the...
What control elements regulate expression of the mPGES-1 gene? The promoter of a gene includes the DNA immediately upstream of the transcription start site, but expression of the gene can also be affected by control elements. These can be thousands of base pairs upstream of the promoter, grouped in an enhancer. Because the distance and spacing of these control elements make them difficult to identify, scientists begin by deleting sections of DNA that contain possible control elements and measuring the...
You wish to determine if there is a mutation somewhere within the promoter of the adult...
You wish to determine if there is a mutation somewhere within the promoter of the adult b-globin gene that could possibly be responsible for a case of b-thalassemia. Which ONE method, when compared to the same process performed on wild type DNA, would NOT provide you with information that could be consistent with this idea? A) Prepare a pair of 18 nucleotide long primers that hybridize upstream and downstream of the promoter, perform PCR, and sequence the resulting fragment B)...
You wish to edit a gene in a population of stem cells using CRISPR/Cas9. You design...
You wish to edit a gene in a population of stem cells using CRISPR/Cas9. You design a plasmid and transfect it into the cells. Your plasmid included the guide RNA sequence with promoter, the Cas9 gene inserted between an appropriate promoter and termination sequence, and the usual Origin of Replication and Resistance/Marker gene. However, instead of being edited, your target gene is silenced and its protein is not produced at all. What is the most likely explanation? The cells have...
Why would you silence gene expression for both wild-type and mutants? I am on a question...
Why would you silence gene expression for both wild-type and mutants? I am on a question that asks why a researcher would use shRNA to reduce the expression of endogenous SRP on both wild-type and mutant SRPs. What would it help test for translational pausing in co-translational import?
when a bear is exposed to radiation, some of its cells can develop mutations in their...
when a bear is exposed to radiation, some of its cells can develop mutations in their DNA. If an adult bear's cell in G, is exposed to radiation, what is most likely going to happen to that cell? a. How might this impact the bear's future health? 8. If an adult bear's call that is dividing to become new skin cells or new lung cells is exposed to radiation and picks up mutations in its DNA, what could happen to...
IN CONTEXT OF GENE EXPRESSION AND PROTEIN SYNTHESIS OUTCOME 5. a), Why are Proto-Oncogenes important for...
IN CONTEXT OF GENE EXPRESSION AND PROTEIN SYNTHESIS
OUTCOME
5. a), Why are Proto-Oncogenes important for the cell cycle control and what is their implication in a progression of tumors and cancers? Provide examples of TWO proto-oncogenes and a type of a cancer each is associated with. (6 pts) b) Now consider the diagram below. Under each of three scenarios, explain a likely cellular consequence relative to gene expression and protein synthesis outcome, assuming each could lead to a development...
someone please help! i have a brain fart :/ tations and Regulation of Gene Expression 6....
someone please help! i have a brain fart :/
tations and Regulation of Gene Expression 6. Which of the following statements about the DNA in your brain cells is TRUE? a. The majority of the DNA in these cells is made up of genes that code for proteins. b. The majority of genes in these cells are expressed as RNA or proteins. C. Your brain cells contain approximately the same DNA as your heart cells. d. All of the above...
Shown below are the amino acid sequences of the wild-type and three mutant forms of a short protein. Each mutation results from a single nucleotide change (transition/transversion / insertion / deletion). Use this information to answer the following questions. Hint: First, reconstruct as much as you can of the wild-type RNA sequence and then reference that sequence when analyzing the mutations. Wild type: met - gin-ala - ser-val - arg - phe Mutant 1: met - gln - pro-ser -...
Question 10 A human chromosome has 3 origins of replication. Assuming that all 3 origins of replication are used at the same time, what is the maximum number of helicases present on DNA during DNA replication (not including DNA repair)? 12 O 3 O2 Question 11 Which of the following correctly compares primase and telomerase? O Both primase and telomerase synthesize DNA using a DNA template. O Primase synthesizes RNA using an RNA template, but telomerase synthesizes DNA using a...
You wish to edit a gene in a population of stem cells using CRISPR/Cas9. You design a plasmid and transfect it into the cells. Your plasmid included the guide RNA sequence with promoter, the Cas9 gene inserted between an appropriate promoter and termination sequence, and the usual Origin of Replication and Resistance/Marker gene. However, instead of being edited, your target gene is silenced and its protein is not produced at all. What is the most likely explanation? The cells have...
when a bear is exposed to radiation, some of its cells can develop mutations in their DNA. If an adult bear's cell in G, is exposed to radiation, what is most likely going to happen to that cell? a. How might this impact the bear's future health? 8. If an adult bear's call that is dividing to become new skin cells or new lung cells is exposed to radiation and picks up mutations in its DNA, what could happen to...
IN CONTEXT OF GENE EXPRESSION AND PROTEIN SYNTHESIS
OUTCOME
5. a), Why are Proto-Oncogenes important for the cell cycle control and what is their implication in a progression of tumors and cancers? Provide examples of TWO proto-oncogenes and a type of a cancer each is associated with. (6 pts) b) Now consider the diagram below. Under each of three scenarios, explain a likely cellular consequence relative to gene expression and protein synthesis outcome, assuming each could lead to a development...
someone please help! i have a brain fart :/
tations and Regulation of Gene Expression 6. Which of the following statements about the DNA in your brain cells is TRUE? a. The majority of the DNA in these cells is made up of genes that code for proteins. b. The majority of genes in these cells are expressed as RNA or proteins. C. Your brain cells contain approximately the same DNA as your heart cells. d. All of the above...
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