13. a. What is/are the function(s) of SDS (sodium dodecyl phosphate) when preparing protein samples for...
13. a. What is/are the function(s) of SDS (sodium dodecyl phosphate) when preparing protein samples for PAGE?
b. What is the function of 2-mercaptoethanol when preparing protein samples for PAGE?
Homework Answers
13. a. SDS disrupts the tertiary structure of proteins and thereby brings the folded proteins down to linear molecules. It is also denatures the secondary protein and gives negative charge to all proteins.
SDS is a anionic detergent, that is present in the SDS-PAGE sample buffer to break down protein-protein disulphide bonds.
b. 2-mercaptoethanol is used to reduce disulphide bonds and thereby irreversibly denatures the protein. That is, it disrupt the disulfide bonds found between the protein complexes, which helps further denaturation.
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SDS Page Gel: The provided standard protein sample for electrophoresis consists of 9 polypeptides with molecular...
SDS Page Gel:
The provided standard protein sample for electrophoresis
consists of 9 polypeptides with molecular weights ranging from 250
to 15 KDa.
Sample 1: Protein A in a sample buffer with
B-Mercaptoethanol
Sample 2: Protein A in a sample buffer without
B-Mercaptoethanol
Sample 3: Protein B in a sample buffer with
B-Mercaptoethanol
Sample 4: Protein C in a sample buffer without
B-Mercaptoethanol
Use the picture below & the information about the proteins
above to answer the following questions.
1a....
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parts a,b, c please
3. Sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis a. After pouring the SDS polyacrylamide gel, you realize that instead of 2 ml, you used 4 ml of 30% acrylamide/bisacrylamide solution for the preparation of the separating gel (in both cases for 5 ml, total gel volume). How would this affect the separation of your proteins during SDS PAGE? Explain your answer! b. You have to remake the gel and are now making sure just to add...
SDS Page Gel:
The provided standard protein sample for electrophoresis
consists of 9 polypeptides with molecular weights ranging from 250
to 15 KDa.
Sample 1: Protein A in a sample buffer with
B-Mercaptoethanol
Sample 2: Protein A in a sample buffer without
B-Mercaptoethanol
Sample 3: Protein B in a sample buffer with
B-Mercaptoethanol
Sample 4: Protein C in a sample buffer without
B-Mercaptoethanol
Use the picture below & the information about the proteins
above to answer the following questions.
1a....
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