CALCULATION: obtain a final Amp concentration of 100 μg/ml in 200ml of pre-warmed LB using a 100 mg/ml stock of ampicillin. CALCULATION: obtain a final Amp concentration of 100 μg/ml in 200ml of...

Question

Question

CALCULATION: obtain a final Amp concentration of 100 μg/ml in 200ml of pre-warmed LB using a 100 mg/ml stock of ampicillin.

Answer 1

Answer #1

Initial stock concentration of Ampicillin, C1 = 100 mg/ml = 100 x 10³ ug/ml = 10⁵ ug/ml

Initial volume, V1 = V ml

Final concentration, C2 = 100 ug/ml

Final volume, V2 = 200 ml

C1V1 = C2V2

10⁵ ug/ml x V = 100 ug/ml x 200 ml

V = 0.2 ml

Volume of initial stock solution = 0.2 ml

0.2 ml of 100 mg/ml is taken and diluted to 200 ml using pre warmed LB agar to obtain final ampicillin concentration of 100 ug/ml

Answer 2

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