how can ion exchange chromatography be improved?
how can ion exchange chromatography be improved?
Homework Answers
Could you please explain how ion exchange chromatography works and how the anion exhange chromatography relates?...
Could you please explain how ion exchange chromatography works and how the anion exhange chromatography relates? Thanks!
Ion Exchange Chromatography Experiment 1. What is the basis for the seperation of different compounds by...
Ion Exchange Chromatography Experiment 1. What is the basis for the seperation of different compounds by ion exchange? 2. How can molecules with the same charge at varying amounts be seperated by chromatography? 3. Why are celluloses often used as supports to seperate large biologically active proteins? 4. Why is it important to prepare a standard curve for each spectrophotometer? 5. What would happen if 0.5M K+ acetate were used to elute the sample? Why? (0.01M KOAc was actually used)
How would you use ion exchange chromatography to seperate a mixture of alanine, glutamic acid, and...
How would you use ion exchange chromatography to seperate a mixture of alanine, glutamic acid, and lysine if you wished to maintain the pH at 7.5 and use only a single ion exchange column ( either an anionic or cationic exchanger). Describe the expected order of elution under the conditions which you propose.
chromatography mechanisms -In ion-exchange mechanism of chromatography, let assume we use cation exchangers, cation will attract...
chromatography mechanisms
-In ion-exchange mechanism of chromatography, let assume we use cation exchangers, cation will attract to stationary phase and remain binded untill we change th PH to get that cation my question is what I do with PH, raised it decrease it to get the cation? and why? -the same for the covalently binding of protein and antigen in affinity chromatography mechanism, how can I get the protein from antigen? is it by using PH (increa decrease)? of course,...
Write 10 multiple choice questions with the answers on Ion exchange Chromatography
Write 10 multiple choice questions with the answers on Ion exchange Chromatography
5) Ion exchange chromatography is based on the A) electrostatic attraction B) electrical mobility of ionic...
5) Ion exchange chromatography is based on the A) electrostatic attraction B) electrical mobility of ionic species C) adsorption chromatography D) partition chromatography
i. Chromatography scale up by a factor of 100 for a linear gradient ion exchange chromatography...
i. Chromatography scale up by a factor of 100 for a linear gradient ion exchange chromatography of a product protein from the laboratory to the plant. The conditions are 2 cm (in diameter) x 20 cm bed height, 20 um particle size and 30 cm/h superficial velocity. The particle size of the same type of ion exchange resin available for the plant operation is 60 um. Two columns are available in the plant: 14 cm in diameter x 50 cm...
To seperate protein X using ion exchange chromatography, I am using a resin with carboxylates attached...
To seperate protein X using ion exchange chromatography, I am using a resin with carboxylates attached (pKa is 6.0). If I wanted to seperate protein X, whose pl is 3.0x what is the pH range of the buffer that can be used for ion exchange chromatography with this resin ? A. any pH between 3.0 and 6.0 B. there is no pH range for this resin and protein X C. any pH above 4.0 D. any pH below 6.0
Ion-interaction chromatography can be used to separate out ions.(i) Discuss this mode of chromatography in terms...
Ion-interaction chromatography can be used to separate out ions.(i) Discuss this mode of chromatography in terms of retention mechanisms, ion-pairing reagents, column type and mobile phase pH requirements.(ii) Describe how this method differs from ion-suppression chromatography.
discuss 1- the advantages and disadvantages of ion exchange chromatography( IEC ) compared to reversed phase...
discuss 1- the advantages and disadvantages of ion exchange chromatography( IEC ) compared to reversed phase liquid chromatography and use specific examples from the literature. 2- specify some samples that you could separate with IEC that you couldn't separate with reverse phase liquid chromatography .
chromatography mechanisms
-In ion-exchange mechanism of chromatography, let assume we use cation exchangers, cation will attract to stationary phase and remain binded untill we change th PH to get that cation my question is what I do with PH, raised it decrease it to get the cation? and why? -the same for the covalently binding of protein and antigen in affinity chromatography mechanism, how can I get the protein from antigen? is it by using PH (increa decrease)? of course,...
5) Ion exchange chromatography is based on the A) electrostatic attraction B) electrical mobility of ionic species C) adsorption chromatography D) partition chromatography
i. Chromatography scale up by a factor of 100 for a linear gradient ion exchange chromatography of a product protein from the laboratory to the plant. The conditions are 2 cm (in diameter) x 20 cm bed height, 20 um particle size and 30 cm/h superficial velocity. The particle size of the same type of ion exchange resin available for the plant operation is 60 um. Two columns are available in the plant: 14 cm in diameter x 50 cm...
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