Question 20-28 asks you to calculate the annealing temperature for a primer in a PCR exp...

Question 20-28 asks you to calculate the annealing temperature for a primer in a PCR experiment. To do this you need to consider the %GC in a primer. Based on what you know about the complementary base pairs, why is annealing temperature dependent on %AT and %GC in a primer?

HINT: This problem involves an understanding of the relation-ship between hybridization temperature in a PCR reaction and the nucleotide composition of PCR primers. The key to its solution is to consider what you know about hydrogen bonding arrangements of complementary base pairs in DNA.

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