Question 20-14 refers to creating a genomic DNA library from the African okapi. If you were the first person in the world attempting to use PCR to amplify particular genes from this library, what strategies might you use w de-sign PCR primers for your experiments if the okapi genome has yet to be sequenced?
HINT: This problem asks you to design Pa primers to amplify the /3-globin gene from a species whose genome you just sequenced. The key to its solution is to remember that you have at your disposal sequence data for the human f3-globin gene and consider that PCR experiments require the use of primers that bind to complementary bases in the DNA to be amplified.
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