A colleague provided you with a cDNA library of human genes cloned into bacterial plasmid vectors. Armed with the cloned yeast gene that encodes EF-la, a protein that is highly conserved among eukaryotes, you use the yeast gene as a probe to isolate a cDNA clone containing the human EF-la gene. You sequence the human gene and find that it is 1384 nucleotide pairs long. Using this cDNA clone as a probe, you isolate the DNA encoding EF- 1a from a human genomic library. The genomic clone is sequenced and found to be 5282 nucleotides long. What accounts for the difference in length observed between the cDNA clone and the genomic clone?
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