For question 20-16, you are given a cDNA library of human genes prepared in a bacterial plasmid vector. You are also given the cloned genomic DNA sequence yeast gene that encodes EF-1 a, a protein that is highly conserved among eukaryotes. Outline how you would use these re-sources to identify the human cDNA clone encoding EF-1 a.
HINT: This problem asks you to use a cDNA library to isolate the human EF-1 a gene when you have access to the yeast EF-1 a gene. The key to its solution is to remember that eDNA clones do not have all the sequences of a genomic library, but do contain coding sequences (exons) and they can be identified with the proper probe.
the basic methods of recombinant DNA technology were the foundation for the development of powerful techniques for whole-genome cloning and sequencing, which led to the genomics era of modern genetics and molecular biology. Increasingly, genomic techniques, in which entire genomes are being sequenced without creating libraries, are replacing many traditional recombinant DNA approaches that cloned or identified individual or a few genes at a time.
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