Problem

Assume you have conducted a DNA sequencing reaction using the chain-termination (Sanger) m...

Assume you have conducted a DNA sequencing reaction using the chain-termination (Sanger) method. You performed all the steps correctly and electrophoresced the resulting DNA fragments correctly, but when you looked at the sequencing gel, many of the bands were duplicated (in terms of length) in other lanes. What might have happened?

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