A fellow student considers the issues in Problem and argues that there is a more straightf...

A fellow student considers the issues in Problem and argues that there is a more straightforward, nongenetic experiment that could differentiate between the two types of mutations. The experiment requires no fancy genetics and would allow you to easily assay the products of the other SOS genes. Propose such an experiment.

The SOS repair genes in E. coli (discussed in Chapter 15) are negatively regulated by the lexA gene product, called the LexA repressor. When a cell sustains extensive damage to its DNA, the LexA repressor is inactivated by the recA gene product (RecA), and transcription of the SOS genes is increased dramatically.

One of the SOS genes is the uvrA gene. You are a student studying the function of the uvrA gene product in DNA repair. You isolate a mutant strain that shows constitutive expression of the UvrA protein. Naming this mutant strain uvrAC, you construct the following diagram showing the lexA and uvrA operons:

(a) Describe two different mutations that would result in a uvrA constitutive phenotype. Indicate the actual genotypes involved.

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(b) Outline a series of genetic experiments that would use partial diploid strains to determine which of the two possible mutations you have isolated.