Consider the regulation of the tip operon in B. subtilis. For each of the following cases, indicate whether the structural genes in the operon are being expressed or not expressed:
In each case, also indicate whether AT is present. Describe the role of TRAP and AT in attenuation.
¦ H I NT: This problem concerns the regulation of the B. subtilis tip operon, which involves the TRAP protein and requires you to under-stand the concept of attenuation. You are asked to predict whether or not the structural genes are expressed under various conditions. The key to its solution is to realize that unlike E. coli, which uses tryptophan as a repressor along with the process of attenuation in regulating the tip operon, B. subtilis relies solely on the process of attenuation and the TRAP protein for regulation of this operon.
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