A marine bacterium is isolated and shown to contain an inducible operon whose genetic products metabolize oil when it is encountered in the environment. Investigation demonstrates that the operon is under positive control and that there is a reg gene whose product interacts with an operator region (o) to regulate the structural genes, designated sg.
In an attempt to understand how the operon functions, a constitutive mutant strain and several partial diploid strains were isolated and tested with the results shown here:
Host Chromosome | F′ Factor | Phenotype |
wild type | none | inducible |
wild type | reg gene from mutant strain | inducible |
wild type | operon from mutant strain | constitutive |
mutant strain | reg gene from wild type | constitutive |
Draw all possible conclusions about the mutation as well as the nature of regulation of the operon. Is the constitutive mutation in the trans-acting reg element or in the cis-acting o operator element?
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