A differential staining procedure works on the principle that Different types of cells will take up...
A differential staining procedure works on the principle that
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All types of cells take up stain equally |
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Different cells will appear the same after staining |
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Staining is the only way to identify bacteria |
Homework Answers
Answer: Different types of cells will take up stain differently.
( The differential staining procedure is based on the principle that the cell wall of the bacteria appears in different colors. The gram negative and positive bacteria can be easily identified.)
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A differential staining procedure works on the principle
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Different types of cells will take up...
Exercise 8 - Gram Staining Procedure for gram staining. Know all chemicals/reagents used in correct order,...
Exercise 8 - Gram Staining Procedure for gram staining. Know all chemicals/reagents used in correct order, function of each chemical, the effect of cach chemical and how gram + and gram - bacteria would appear at each step Color reactions at each step of procedure. Know why, based on the differences in cell wall structure, that the gram + vs gram - bacteria stain either purple or pink. What washes off in the decolorizing step of a gram - bacteria?...
microbiology QUESTION 7 Procedure for Differential Staining uses OA Two Basic Dyes OB. An Acidic Dye...
microbiology
QUESTION 7 Procedure for Differential Staining uses OA Two Basic Dyes OB. An Acidic Dye & a Basic Dye O C. Neutral dye with two colored ions O D. Acidic Dyes with contrasting colors QUESTION 8 commonly Both Clostridium species and Bacillus species types of bacteria are co OA Rod-shaped O B. Endospore-producers O C. Gram positive but older cultures appear negative O D. All of the above QUESTION 9 Both Mycobacterium tuberculosis and Coxiella burnetii are A. Gram...
NEED HELP WITH ALL PLEASE. MICROBIOLOGY You have stained a bacteria using the Gram staining technique...
NEED HELP WITH ALL PLEASE. MICROBIOLOGY
You have stained a bacteria using the Gram staining technique what color would any endospores be if present? A. Colorless B. Green C. Pink D. Blue E. Purple 2. If you omitted the Safranin step in a gram staining procedure, what would be the color of a Gram-negative bacteria? A. Colorless B. Pink C. Green D. Purple E. Blue 3. Bacillus licheniformis would be what color and shape after a Gram staining? A. Purple...
In their Portland, Oregon facility, Wavy Wood Works makes several types of wood bowls from coastal drift wood. To make...
In their Portland, Oregon facility, Wavy Wood Works makes several types of wood bowls from coastal drift wood. To make a bowl, the first step is to clean the drift wood. Each piece of drift wood makes a single bowl. There is one cleaning machine. It takes 38 seconds to load each piece of wood and the machine can hold up to 33 pieces. Once all of the pieces are loaded into the machine, it takes 44 minutes to clean...
In humans, there are about 200 different types of cells. Why are your liver cells different...
In humans, there are about 200 different types of cells. Why are your liver cells different from skin cells, or neurons, or muscle cells? During development, each cell accumulates different mutations changing their DNA They produce the same proteins but some of those proteins are denatured in each cell They have different DNA and thus, each cell produces different proteins They produce the same kind of proteins but not all proteins are active in each cell They have the same...
Which statement about gene cloning is false? A. Bacterial cells take up recombinant plasmids by transformation....
Which statement about gene cloning is false? A. Bacterial cells take up recombinant plasmids by transformation. B. Recombinant DNA molecules usually contain a DNA fragment inserted into a bacterial vector. C. To insert a gene of interest into a vector, the gene of interest and the vector must be digested with different restriction enzymes D. Transformed bacteria are plated onto media containing the appropriate antibiotic and only bacteria containing the plasmid with antibiotic resistance will grow.
5. Why are simple stains able to "stick” to bacteria? Explain the chemical properties of the...
5. Why are simple stains able to "stick” to bacteria? Explain the chemical properties of the stain and bacteria surface. 3-Preparation of Bacterial Smears and Simple Stains Introduction Microbial cultures are handled using aseptic technique. Aseptie technique is a set of procedures used to minimize contamination of materials by microorganisms from the environment, and to minimize the spread of cultures around the laboratory. In this experiment, you will be using an inoculating loop to transfer bacteria from a culture tube...
BACKGROUND: Mycobacterium tuberculosis causes tuberculosis, one of the world’s deadliest diseases...
BACKGROUND:
Mycobacterium tuberculosis causes tuberculosis, one of the world’s
deadliest diseases. It is an acid-fast bacillus that is 2-4 um in
length and 0.2-0.5 um in width.
How many weeks, days,& hours would it take to see a
M.tuberculosis colony on solid media? Its generation time is listed
in the reading (above). Assume that bacterial colonies are not
visible to the naked eye unless they contain ≥ 2 millioncells. This
species forms aggregates of 5 cells on average. Show your...
A sentence or two stating the hypothesis or hypotheses made. A sentence or two describing what...
A sentence or two stating the hypothesis or hypotheses
made.
A sentence or two describing what was done
A summary of the most important results
A sentence stating the major conclusion
EXPERIMENT 2: CHARACTERIZING BACTERIA ISOLATED FROM SOIL Data Tables Table 1: Gram Staining Observations Sample Colony 1 Colony 2 Colony 3 + "Normal" + "Heated" Table 2: Experiment 2b Catalase Assay Results Colony Catalase Assay Results ("Normal") Catalase Assay Results ("Heated') 1 2 3 + Characterizing Bacteria Isolated from...
Procedure Four different colors of beads, representing four species of bacteria, all possible residents of the...
Procedure Four different colors of beads, representing four species of bacteria, all possible residents of the microbiome, will be utilized. The species differ naturally in their resistance to a particular antibiotic. Three different antibiotic treatment regimens will be modeled: following doctor’s orders ending antibiotic treatment early skipping Day 3 of treatment Your initial population of bacteria is represented as follows: 40 beads that represent Species 1, the most susceptible to the antibiotic (susceptible on Day 1 of treatment) 30 beads...
Exercise 8 - Gram Staining Procedure for gram staining. Know all chemicals/reagents used in correct order, function of each chemical, the effect of cach chemical and how gram + and gram - bacteria would appear at each step Color reactions at each step of procedure. Know why, based on the differences in cell wall structure, that the gram + vs gram - bacteria stain either purple or pink. What washes off in the decolorizing step of a gram - bacteria?...
microbiology
QUESTION 7 Procedure for Differential Staining uses OA Two Basic Dyes OB. An Acidic Dye & a Basic Dye O C. Neutral dye with two colored ions O D. Acidic Dyes with contrasting colors QUESTION 8 commonly Both Clostridium species and Bacillus species types of bacteria are co OA Rod-shaped O B. Endospore-producers O C. Gram positive but older cultures appear negative O D. All of the above QUESTION 9 Both Mycobacterium tuberculosis and Coxiella burnetii are A. Gram...
NEED HELP WITH ALL PLEASE. MICROBIOLOGY
You have stained a bacteria using the Gram staining technique what color would any endospores be if present? A. Colorless B. Green C. Pink D. Blue E. Purple 2. If you omitted the Safranin step in a gram staining procedure, what would be the color of a Gram-negative bacteria? A. Colorless B. Pink C. Green D. Purple E. Blue 3. Bacillus licheniformis would be what color and shape after a Gram staining? A. Purple...
In their Portland, Oregon facility, Wavy Wood Works makes several types of wood bowls from coastal drift wood. To make a bowl, the first step is to clean the drift wood. Each piece of drift wood makes a single bowl. There is one cleaning machine. It takes 38 seconds to load each piece of wood and the machine can hold up to 33 pieces. Once all of the pieces are loaded into the machine, it takes 44 minutes to clean...
In humans, there are about 200 different types of cells. Why are your liver cells different from skin cells, or neurons, or muscle cells? During development, each cell accumulates different mutations changing their DNA They produce the same proteins but some of those proteins are denatured in each cell They have different DNA and thus, each cell produces different proteins They produce the same kind of proteins but not all proteins are active in each cell They have the same...
5. Why are simple stains able to "stick” to bacteria? Explain the chemical properties of the stain and bacteria surface. 3-Preparation of Bacterial Smears and Simple Stains Introduction Microbial cultures are handled using aseptic technique. Aseptie technique is a set of procedures used to minimize contamination of materials by microorganisms from the environment, and to minimize the spread of cultures around the laboratory. In this experiment, you will be using an inoculating loop to transfer bacteria from a culture tube...
BACKGROUND:
Mycobacterium tuberculosis causes tuberculosis, one of the world’s
deadliest diseases. It is an acid-fast bacillus that is 2-4 um in
length and 0.2-0.5 um in width.
How many weeks, days,& hours would it take to see a
M.tuberculosis colony on solid media? Its generation time is listed
in the reading (above). Assume that bacterial colonies are not
visible to the naked eye unless they contain ≥ 2 millioncells. This
species forms aggregates of 5 cells on average. Show your...
A sentence or two stating the hypothesis or hypotheses
made.
A sentence or two describing what was done
A summary of the most important results
A sentence stating the major conclusion
EXPERIMENT 2: CHARACTERIZING BACTERIA ISOLATED FROM SOIL Data Tables Table 1: Gram Staining Observations Sample Colony 1 Colony 2 Colony 3 + "Normal" + "Heated" Table 2: Experiment 2b Catalase Assay Results Colony Catalase Assay Results ("Normal") Catalase Assay Results ("Heated') 1 2 3 + Characterizing Bacteria Isolated from...
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