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-). You have a specimen in focus on the 40X objective magnification. Detail the steps needed to view the specimen at 100X obj

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Answer #1

1. For observing the sample in 100x put 1-2 drops of immersion oil, for example, cedar oil, on the coverslip.

2. Rotate the diaphragm to the 100x lens. Now the space between the lens and specimen is covered by the oil and there is no air left. Air has a low refractive index than glass and thus the light rays bend when passing from glass to air. This can disturb the clarity of the specimen. Thus immersion oil is added. Moreover, it also reduces the friction and breakage of the glass coverslip and gives a clear and detailed view of the specimen.

3. Adjust the coarse knob to get an image of the specimen.

4. Then do the fine focusing with a fine focusing knob.

5. After observing the sample clean the lens with a tissue paper and with a solution such as a xylene.

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