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please answer this question
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1 QUESTION...
NEED HELP WITH THESE QUESTIONS. PLEASE ANSWER ALL AND EXPLAIN AS WELL. THANKSSSSSSS 1. You want...
NEED HELP WITH THESE QUESTIONS. PLEASE ANSWER ALL AND EXPLAIN AS
WELL. THANKSSSSSSS
1. You want to clone a gene from a donor vector to a host vector. List the correct order of events in the process of cloning a. Perform ligation reaction of cloned gene and host vector. b. Perform double digestion of both donor and host vectors with the 2 restriction enzymes c. Examine donor and host vectors for restriction sites d. Purify cloned gene from donor vector...
*Microbiology. Please answer the below question. The current selected answer is incorrect. Thank you! Incorrect 0/1...
*Microbiology. Please answer the below question. The current
selected answer is incorrect. Thank you!
Incorrect 0/1 pts Question 1 Which of the following PCR components serves as the template? a. Target gene sequence • b. Synthetic primers c. Deoxyribonucleotides d. DNA polymerase III
Please help me with these questions. Thank u very much. Q1: A DNA sample from an...
Please help me with these questions. Thank u very much. Q1: A DNA sample from an individual is analyzed. The population frequency of one of the STR alleles is 1 in 50, a second STR allele is 1 in 100, and a third STR allele is 1 in 30. What is the combined frequency of these three STR alleles? a.1 in 150,000 b.1 in 15,000 c.1 in 1,500 d.1 in 180 e.1 in 50 Q2: Which of the following hybridize...
How to solve for question#6? 5 5. Fill in the table below to set up the...
How to solve for question#6?
5 5. Fill in the table below to set up the reactions for a single and then a set of PCR reactions: Concentration in Volume in 1 Master Mix for four Reagent Stock concentration 10X 25 mM one reaction 1X 2 mM reaction 25 jl reactions Buffer MgClz dNTPs Primer mix DNA template Taq polymerase water Total volume 2.SA 2 al 2.S A Pe 100 nM-0.IA 2 ng/HI 1 unit VS 10 μΜ 0.25 l...
Question 1: As a researcher, you want to prevent gene expression by cleaving the product of...
Question 1: As a researcher, you want to prevent gene expression by cleaving the product of transcription. Which regulatory mechanism would you use? a, miRNA b. siRNA c. miRNA or siRNA d. proteasome e. RNA splicing ------------------------------------------------------------------------------------------------------------------- Question 1a: Which statement represents a difference between DNA replication in your cells and amplification through PCR? a. All of the above represents differences b. DNA primers are required for PCR, but not in your cells c. PCR involves differential temperature, but your...
QUESTION 1: You are inserting a gene into an MCS found within the LacZ gene. Using...
QUESTION 1: You are inserting a gene into an MCS found within the LacZ gene. Using blue/white colony selection, why could you assume that white colonies have modified plasmids? a. A blue colony means the LacZ reading-frame was disrupted b. A blue colony means your gene has mutations c. A white colony means the LacZ reading-frame is intact d. A white colony means the LacZ reading-frame was disrupted QUESTION 2: You are performing a PCR using primers with a sequence perfectly...
please all them Using the picture below, "G" represents a factor that fills in a small...
please all them
Using the picture below, "G" represents a factor that fills in a small gap between two DNA fragments by “sealing up" the phosphodiester backbone. This factor is called a: А A) single stranded binding protein B) ligase C) topoisomerase O A) single stranded binding protein B) ligase C) topoisomerase D) DNA polymerase III E) helicase Which of the lists below might contain sufficient ingredients to allow D. replication to occur in a test tube? A) primers, template...
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain...
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain reaction? a. To repair damaged DNA b. To make copies of entire chromosomes c. To make copies of specific regions of DNA d. To prepare cells for cell division 2. The polymerase chain reaction is most comparable to what cellular process? a. Mitosis b. Replication c. Transcription d. Translation 3. When enzymes are elongating (building) a newly synthesized DNA strand in PCR, new nucleotides...
*Microbiology. Please answer the below two multiple choice questions. The current selected answers are incorrect. Thank...
*Microbiology. Please answer the below two multiple choice
questions. The current selected answers are incorrect. Thank you,
will provide positive feedback for correct answers!
Incorrect Question 3 0/1 pts The replication fork has both leading (continuous) and lagging (discontinuous) synthesis because: a. The origin of replication only allows DNA synthesis to occur in one direction around the chromosome. b. Primase only works on the leading strand c. Helicase is slow in unwinding the double helix so the polymerization moving toward...
alllll them please all To MOST readily demonstrate transformation of bacteria in the laboratory one could...
alllll them please all
To MOST readily demonstrate transformation of bacteria in the laboratory one could extract DNA from: A) his cells and add the DNA to his cells, then grow the cells on plates with histidine B) hist cells and add the DNA to his cells, then grow the cells on plates without histidine C) lac" cells and add the DNA to lact cells, then grow the cells on plates without histidine D) amp cells and add the DNA...
NEED HELP WITH THESE QUESTIONS. PLEASE ANSWER ALL AND EXPLAIN AS
WELL. THANKSSSSSSS
1. You want to clone a gene from a donor vector to a host vector. List the correct order of events in the process of cloning a. Perform ligation reaction of cloned gene and host vector. b. Perform double digestion of both donor and host vectors with the 2 restriction enzymes c. Examine donor and host vectors for restriction sites d. Purify cloned gene from donor vector...
*Microbiology. Please answer the below question. The current
selected answer is incorrect. Thank you!
Incorrect 0/1 pts Question 1 Which of the following PCR components serves as the template? a. Target gene sequence • b. Synthetic primers c. Deoxyribonucleotides d. DNA polymerase III
How to solve for question#6?
5 5. Fill in the table below to set up the reactions for a single and then a set of PCR reactions: Concentration in Volume in 1 Master Mix for four Reagent Stock concentration 10X 25 mM one reaction 1X 2 mM reaction 25 jl reactions Buffer MgClz dNTPs Primer mix DNA template Taq polymerase water Total volume 2.SA 2 al 2.S A Pe 100 nM-0.IA 2 ng/HI 1 unit VS 10 μΜ 0.25 l...
please all them
Using the picture below, "G" represents a factor that fills in a small gap between two DNA fragments by “sealing up" the phosphodiester backbone. This factor is called a: А A) single stranded binding protein B) ligase C) topoisomerase O A) single stranded binding protein B) ligase C) topoisomerase D) DNA polymerase III E) helicase Which of the lists below might contain sufficient ingredients to allow D. replication to occur in a test tube? A) primers, template...
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain reaction? a. To repair damaged DNA b. To make copies of entire chromosomes c. To make copies of specific regions of DNA d. To prepare cells for cell division 2. The polymerase chain reaction is most comparable to what cellular process? a. Mitosis b. Replication c. Transcription d. Translation 3. When enzymes are elongating (building) a newly synthesized DNA strand in PCR, new nucleotides...
*Microbiology. Please answer the below two multiple choice
questions. The current selected answers are incorrect. Thank you,
will provide positive feedback for correct answers!
Incorrect Question 3 0/1 pts The replication fork has both leading (continuous) and lagging (discontinuous) synthesis because: a. The origin of replication only allows DNA synthesis to occur in one direction around the chromosome. b. Primase only works on the leading strand c. Helicase is slow in unwinding the double helix so the polymerization moving toward...
alllll them please all
To MOST readily demonstrate transformation of bacteria in the laboratory one could extract DNA from: A) his cells and add the DNA to his cells, then grow the cells on plates with histidine B) hist cells and add the DNA to his cells, then grow the cells on plates without histidine C) lac" cells and add the DNA to lact cells, then grow the cells on plates without histidine D) amp cells and add the DNA...
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