Question

1. Use Figure 10 as a reference to construct a strand of Pop-it® beads with the same color pattern. This is your DNA strand. 5 3' 00000000000000000000000000000000000 Figure 10: Nucleotide sequence. Note the directionality of the DNA strand. 2. Use "Enzyme 1" to cut the strand of DNA based on the information provided in Table 1. Then, record your data in Table 1. 3. Repeat this procedure for each enzyme. Note: The bottom row titled "Enzyme 2 then 3" means that Enzyme 2 fragments the DNA first, then Enzyme 3 cuts the fragments made by Enzyme 2.

Answer 1

Answer: Enzyme 1: 3 fragments. Sizes: 23 nucleotides long, 9 nucleotides long and 4 nucleotides long.

Enzyme 2: 3 fragments. Sizes: 17 nucleotides long, 11 nucleotides long, 7 nucleotides long.

Enzyme 3: 6 fragments. Sizes: 5 nucleotides long, 3 nucleotides long, 5 nucleotides long, 10 nucleotides long, 7 nucleotides long, 5 nucleotides long.

Enzyme 4: 4 fragments. Sizes: 2 nucleotides long, 8 nucleotides long, 24 nucleotides long, 1 nucleotide long.

Enzyme 5: 9 fragments. 5, 10, 5, 2, 5, 6, 5, 2and 5 nucleotides long.

The enzyme cuts in between the designated places and the number of nucleotides are to be counted in between for the estimation of the sizes.

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