8. Dna is a double helix structure, each necleotide pairs with a complimentary nucleotide via hydrogen bond. Cytosine pairs with Guanine and Thymine pairs with Adenine. For a DNA template, 5- CT -3', it will pair with 3'- GA -5'. The structure of DNA will be
The dotted lines the figure represents hydrogen bond.
9. The restriction enzyme has restriction site of 5'- GAATTC -3' and it cleaves the DNA strand between A and G.
So, In the given DNA fragment given,
8. Draw chemical structure of a double strand DNA molecule of following DNA template S'CT3. Include...
If you cut the following single stranded DNA fragment with a restriction enzyme with restriction site of 5’GAATTC 3” and the cutting point between G and A. a. How many fragments you will get b. Specify the size (Number of bases) and the sequence of each fragment, pay attention to DNA direction (5’-3’) 5” ACATTGTCCGGGAATTC CGGGCTAGGCAT T GAATTGGAACA GAATTC GGGCCCGATCCGTA 3
If the sequence of the 5'-3'DNA strand is AATGCTAC, then the complementary DNA sequence has the following sequence o 3-AATGCTAC-5' 3'-CATCGTAA-5 3-GTAGCATT-5 3-TTACGATG-5 Question 2 20 pts Which of the following does the enzyme primase make? phosphodiester linkages (bonds) Okazaki fragments RNA primer DNA primer In which direction does DNA replication take place? 3'to 5 5'to 5 5'to 3 3'to 3 Question 4 20 pts Which enzyme unwinds the double-stranded helical DNA starting at the origin of replication? ligase primase...
D Question 1 (Matching) Match the enzyme/structure with its role in DNA replication. DNA polymerase synthesizes the new strand of RNA primase Choose synthesizes the new strand of DNA synthesizes a short fragment of complementary RNA attach to template DNA strands to prevent hydrogen bonding Uncoils the supercoil of prokaryotic chromosomes connects Okazaki fragments of new lagging strand of DNA unwinds and unzips the double stranded DNA Single stranded binding proteins (SSB's) DNA Ligase [Choose] Helicase [Choose) DNA Gyrase (Choose]...
Draw the chemical structure of the DNA trimer 5’-ACG-3’ hydrogen-bonded to its complementary strand. Include the deoxyribose rings and the phosphodiester backbones and show the H-bonding interactions between the bases.
Draw a figure of one double stranded DNA molecule that has initiated replication and produced two okazaki fragment in each lagging strand. The figure must include both template strands, all newly synthesized DNA molecules on both leading and lagging strands, all RNA primers, direction of chain growth for each fragment/strand indicated with arrowheads, direction of replication forks. Each molecule must be labeled with the origin of replication on both strands, leading and lagging strands labeled, template or newly synthesized, DNA...
Use the following DNA sequence as the template strand to answer these questions. (8 points) 5’- GAT CCT GCC TAA -3’ Draw the non-template DNA sequence, the mRNA sequence and the resulting peptide. Label the terminus of the DNA and peptide!! Draw a point mutation. Is this a transition mutation or transverse mutation? Did it change the amino acid sequence(draw out the peptide)? 4 bp insertion, and the resulting peptide. 2 bp deletion, and the resulting peptide. A copy number...
The following diagram shows the chemical structure of double-stranded DNA. 0- i. ii. Label the 5' and 3' ends on each strand. Indicate with an arrow the hydrogen bonds in the structure above. (4 marks) (1 mark) (a) Name the bonding between the adjacent nucleotides on a DNA strand, and (b) give the total number of this bonding in the structure above? (2 marks)
In a long double-stranded DNA molecule containing the genetic information for many genes, the template strand for one gene may be the nontemplate strand for another gene. true or false
Which of the following shows a double-stranded segment of DNA that might be cut by a restriction enzyme? 5’ GACTGACT 3’ 3’ CTGACTGA 5’ 5’ GAGGAG 3’ 3’ CTCCTC 5’ 5’ GAATTC 3’ 3’ CTTAAG 5’
24. What would be the anticodon if the template strand of DNA Is ACC A UCC B.) TGG UGG D. ACC E. TCC 25. Prior to protein synthesis, the DNA A. attracts tRNAs with appropriate amino acids. 6.) serves as a template for the production of mRNA. C. adheres to ribosomes for protein synthesis. D. contains anticodons that become codons. E. must first undergo replication. 26. The Human Genome Project has revealed that human DNA has approximately A. 30,000 bases...