Homework Answers
Standard curve is a reliable way to calculate the uncertainty of concentration calculated from the calibration curve.
The standard curve given in the question is more reliable in p-nitrophenol concentrations of 10 to 30 nmol/ml.
Add Answer to:
Is the standard curve reliable?
What range is most reliable?
Absorbance at 410 0.2 8 100...
In the standard curve for nitrophenol generated for use in this experiment, what is reported on...
In the standard curve for nitrophenol generated for use in this experiment, what is reported on the x-axis? nitrophenol concentration absorbance at 410 nm initial velocity time QUESTION 4 In the standard curve for nitrophenol generated for use in this experiment, what is reported on the y-axis? initial velocity time nitrophenol concentration absorbance at 410 nm QUESTION 5 The initial velocity of an enzyme catalyzed reaction is determined by graphically determining 1/2 Vmax calculating the amount of product formed over...
You have just created a standard curve of glucose concentration as a function of ANS absorbance...
You have just created a standard curve of glucose concentration as a function of ANS absorbance values. Using the equation of a line (y= 1.0357x), what absorbency is associated with a glucose concentration of of 0.71? y = 1.0357x R2 = 0.9843 Absorbance (540nm) 0 0.2 1 1.2 0.4 0.6 0.8 Glucose Concentration (mg/mL)
To determine the phosphate concentration in a water sample, the following standard curve was produced. If the absorbance of the water sample is 0.030, what is its phosphate concentration? Standard Cu...
To determine the phosphate concentration in a water sample, the following standard curve was produced. If the absorbance of the water sample is 0.030, what is its phosphate concentration? Standard Curve of Known Phosphate Concentrations (M) 0.3 y 1254.6x+0.0189 0.9889 025 0.2 015 0.1 0.05 500L-0S 1000-04 2.500-04 CONCENIRATION IM A. 2.6 x10-5 M VB. 8.8 x 10-6 M C. 3.1 x 104M D. 1.6 x 10-7 M
To determine the phosphate concentration in a water sample, the following standard...
A Bill' external standard curve built from absorbance signal vs. analyte concentration (in uM) has the...
A Bill' external standard curve built from absorbance signal vs. analyte concentration (in uM) has the following calibration curve equation y = 0.0500 x + 0.003. If a sample produced an absorbance of 0.300, what was the Bi concentration in the solution in the cuvette? A. 0.015 mM B. 6.00 mm c. 5.94 mm D. 0.600 mm Reset Selection estion 4 of 5 2 Points If the concentration of Bi in a diluted sample (after dilution of the original sample)...
1. You have generated the following standard curve after a Bradford Assay to measure Absorbance vs...
1. You have generated the following standard curve after a Bradford Assay to measure Absorbance vs samples of known BSA concentrations. Now you need to determine the amount of protein in two mitochondria samples taken from fish, one taken from fish under normal conditions and the other taken from fish under oxidative stress. The absorbance reading for the normal fish fraction was A595=175, and the absorbance reading for the stressed fish fraction was A595= 275 Bradford Assay Standard Curve y...
Calibration Curve Standard Number [Fe mg/ml Absorbance 0.001 0.228 2 0.002 0412 0.003 s. 622 0.004...
Calibration Curve Standard Number [Fe mg/ml Absorbance 0.001 0.228 2 0.002 0412 0.003 s. 622 0.004 0802 esos 0.990 Show example calculation for Fe concentration of standard number 1: 5 L Use Excel to construct the calibration curve. Include the regression line and equation. Submit with your report Mass of unknown Fe sample_0.1026 Sample unknown mass diluted to 50 mL Trial Sample solution Absorbance % Fein sample per trial (mL) Dilution Volume ( 50 50 50 21 31 0,532 6...
I) ion solution of unknown concentration shows an absorbance of 0.55. What a. A metal(II) ion sol...
I) ion solution of unknown concentration shows an absorbance of 0.55. What a. A metal(II) ion solution of unk of the metal(II) ion in the sample? nce is the molar concentration b. Assu ming a molar mass of the metal(II) ion is 58.93 g/mol, what is the concent mg/L (ppm)? what is the concentration of metal(lI) ion in units eFor a metal(II) ion solution having a 0.0080 M concentration, what would be its predicted absorbance 5. Experimental Procedure, Part D....
Spectrophotometry help!? Would measuring the absorbance of the standard solutions used to prepare a calibration curve...
Spectrophotometry help!? Would measuring the absorbance of the standard solutions used to prepare a calibration curve and the unknown sample using a wavelength 10 nm higher or lower than lambda max provide an accurate concentration value for the unknown? Explain. If in an experiment, the pathlength of the light is 1 cm, and the concentration unit of the standard solutions is reported as mM, what would the units of the molar extinction coefficient, ε, be?
1- What is a standard curve and how is it used in biochemistry? 2- Explain how...
1- What is a standard curve and how is it used in biochemistry? 2- Explain how the Lambert-Beer equation is applied in this experiment. 3- Why is a spectrophotometer used to obtain data? 4- Based on the experimental data provided, estimate the amount of amino acid in the given unknown solution by Ninhydrin method. SI No. Amount of amino acid [pg) OD at 570nm 1 2 3 4 5 6 Volume of standard amino acid solution (ml) Blank 0.2 0.4...
PROCEDURE Table 4.1: Dilutions of Albumin for Standard Curve Determination. SAMPLE NUMBER VOLUME OF 0.9% NACL...
PROCEDURE Table 4.1: Dilutions of Albumin for Standard Curve Determination. SAMPLE NUMBER VOLUME OF 0.9% NACL (mL) VOLUME OF 10 mg/ml ALBUMIN STOCK SOLUTION (mL) VOLUME OF BIURET REAGENT (mL) TOTAL VOLUME IN EACH TUBE ABSORBANCE READING ALBUMIN CONCENTRATION IN SOLUTION 1 (Blank) 1.0 mL OmL 4.0 mL 0.8 mL 0.2 mL 4.0 mL .4 0.6 mL 0.4 mL 4.0 mL 5 mL 5ml sme 5mL 5ml 5mL .228 .088 1 0.271 773 1.444 11827 0.4 mL 0.6 mL 4.0...
In the standard curve for nitrophenol generated for use in this experiment, what is reported on the x-axis? nitrophenol concentration absorbance at 410 nm initial velocity time QUESTION 4 In the standard curve for nitrophenol generated for use in this experiment, what is reported on the y-axis? initial velocity time nitrophenol concentration absorbance at 410 nm QUESTION 5 The initial velocity of an enzyme catalyzed reaction is determined by graphically determining 1/2 Vmax calculating the amount of product formed over...
You have just created a standard curve of glucose concentration as a function of ANS absorbance values. Using the equation of a line (y= 1.0357x), what absorbency is associated with a glucose concentration of of 0.71? y = 1.0357x R2 = 0.9843 Absorbance (540nm) 0 0.2 1 1.2 0.4 0.6 0.8 Glucose Concentration (mg/mL)
To determine the phosphate concentration in a water sample, the following standard curve was produced. If the absorbance of the water sample is 0.030, what is its phosphate concentration? Standard Curve of Known Phosphate Concentrations (M) 0.3 y 1254.6x+0.0189 0.9889 025 0.2 015 0.1 0.05 500L-0S 1000-04 2.500-04 CONCENIRATION IM A. 2.6 x10-5 M VB. 8.8 x 10-6 M C. 3.1 x 104M D. 1.6 x 10-7 M
To determine the phosphate concentration in a water sample, the following standard...
A Bill' external standard curve built from absorbance signal vs. analyte concentration (in uM) has the following calibration curve equation y = 0.0500 x + 0.003. If a sample produced an absorbance of 0.300, what was the Bi concentration in the solution in the cuvette? A. 0.015 mM B. 6.00 mm c. 5.94 mm D. 0.600 mm Reset Selection estion 4 of 5 2 Points If the concentration of Bi in a diluted sample (after dilution of the original sample)...
1. You have generated the following standard curve after a Bradford Assay to measure Absorbance vs samples of known BSA concentrations. Now you need to determine the amount of protein in two mitochondria samples taken from fish, one taken from fish under normal conditions and the other taken from fish under oxidative stress. The absorbance reading for the normal fish fraction was A595=175, and the absorbance reading for the stressed fish fraction was A595= 275 Bradford Assay Standard Curve y...
Calibration Curve Standard Number [Fe mg/ml Absorbance 0.001 0.228 2 0.002 0412 0.003 s. 622 0.004 0802 esos 0.990 Show example calculation for Fe concentration of standard number 1: 5 L Use Excel to construct the calibration curve. Include the regression line and equation. Submit with your report Mass of unknown Fe sample_0.1026 Sample unknown mass diluted to 50 mL Trial Sample solution Absorbance % Fein sample per trial (mL) Dilution Volume ( 50 50 50 21 31 0,532 6...
I) ion solution of unknown concentration shows an absorbance of 0.55. What a. A metal(II) ion solution of unk of the metal(II) ion in the sample? nce is the molar concentration b. Assu ming a molar mass of the metal(II) ion is 58.93 g/mol, what is the concent mg/L (ppm)? what is the concentration of metal(lI) ion in units eFor a metal(II) ion solution having a 0.0080 M concentration, what would be its predicted absorbance 5. Experimental Procedure, Part D....
1- What is a standard curve and how is it used in biochemistry? 2- Explain how the Lambert-Beer equation is applied in this experiment. 3- Why is a spectrophotometer used to obtain data? 4- Based on the experimental data provided, estimate the amount of amino acid in the given unknown solution by Ninhydrin method. SI No. Amount of amino acid [pg) OD at 570nm 1 2 3 4 5 6 Volume of standard amino acid solution (ml) Blank 0.2 0.4...
PROCEDURE Table 4.1: Dilutions of Albumin for Standard Curve Determination. SAMPLE NUMBER VOLUME OF 0.9% NACL (mL) VOLUME OF 10 mg/ml ALBUMIN STOCK SOLUTION (mL) VOLUME OF BIURET REAGENT (mL) TOTAL VOLUME IN EACH TUBE ABSORBANCE READING ALBUMIN CONCENTRATION IN SOLUTION 1 (Blank) 1.0 mL OmL 4.0 mL 0.8 mL 0.2 mL 4.0 mL .4 0.6 mL 0.4 mL 4.0 mL 5 mL 5ml sme 5mL 5ml 5mL .228 .088 1 0.271 773 1.444 11827 0.4 mL 0.6 mL 4.0...
Most questions answered within 3 hours.
-
During Heaton Company’s first two years of operations, it
reported absorption costing net operating income as...
asked 9 minutes ago -
Consider testing the
hypotheses:
H0: p = 0.56
H1: p > 0.56
where p is
the...
asked 12 minutes ago -
In a two-factor analysis of variance a main effect is defined as
____.
a. the unique...
asked 19 minutes ago -
A bank's loan officer rates applicants for credit. The ratings
are normally distributed with a mean...
asked 20 minutes ago -
Fill in the following information for Adipic Acid: molecular
weight, g or ml, mole, melting point,...
asked 25 minutes ago -
how
to use a for loop to iterate over the string below and print each
character...
asked 27 minutes ago -
Please type answer, Thanks.
Respond to the following in a minimum of 175
words:
Research industry...
asked 26 minutes ago -
Assume that all interest rates in the economy increase from 9
percent to 10 percent. Which...
asked 36 minutes ago -
Describe the function of a Change Control Board.
When calculating Planned Value (PV), what is the...
asked 42 minutes ago -
The customer help center in your company receives calls from
customers who need help with some...
asked 53 minutes ago -
A manufacturer wished to compare the wearing qualities of two
different types of automobile tires, A...
asked 51 minutes ago -
Write a MATLAB script file that will sum all the positive
entries in V and stop...
asked 1 hour ago